Improvement of the Pour Plate Method by Separate Sterilization of Agar and Other Medium Components and Reduction of the Agar Concentration

Improvement of the Pour Plate Method by Separate Sterilization of Agar and Other Medium Components and Reduction of the Agar Concentration

ABSTRACT Although the pour plate method is widely employed in microbiological quality control, it has certain drawbacks, including having to melt the culture medium before seeding. In this study, the preparation of the culture medium was modified by using a lower concentration of agar (10 g/L), whic...

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Bibliographic Details
Main Authors: I. Terrones-Fernandez, P. Casino, A. López, S. Peiró, S. Ríos, A. Nardi-Ricart, E. García-Montoya, D. Asensio, A. M. Marqués, R. Castilla, P. J. Gamez-Montero, N. Piqué
Format: Article
Language:English
Published: American Society for Microbiology 2023-02-01
Series:Microbiology Spectrum
Subjects:
pour plate method
TSA
SDA
VRBG
recovery
selectivity
Online Access:https://journals.asm.org/doi/10.1128/spectrum.03161-22

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