Evaluation of the in vitro and in vivo antioxidant potential of Punica granatum L. against toluene-induced liver injuries in rats
Background and Aim: Humans are more frequently exposed to chemicals in daily life by inhalation of indoor and outdoor air. However, abusers and workers are the most exposed to those chemicals and their health risks particularly, liver diseases. The present study investigated the protective effects o...
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| Format: | Article |
| Language: | English |
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Veterinary World
2022-02-01
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| Series: | Veterinary World |
| Subjects: | |
| Online Access: | http://www.veterinaryworld.org/Vol.15/February-2022/18.pdf |
| _version_ | 1818293772336758784 |
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| author | Fatma Zohra Arkoub Leila Hamdi Labiba Kahalerras Meriem Hamoudi Kamel Khelili |
| author_facet | Fatma Zohra Arkoub Leila Hamdi Labiba Kahalerras Meriem Hamoudi Kamel Khelili |
| author_sort | Fatma Zohra Arkoub |
| collection | DOAJ |
| description | Background and Aim: Humans are more frequently exposed to chemicals in daily life by inhalation of indoor and outdoor air. However, abusers and workers are the most exposed to those chemicals and their health risks particularly, liver diseases. The present study investigated the protective effects of pomegranate juice (PJ) (Punica granatum) and pomegranate peel aqueous extract (PAE) supplementation against toluene (Tol)-induced hepatotoxicity in Wistar rats.
Materials and Methods: A phytochemical analysis and assessment of the 2,2-diphenyl-1-picrylhydrazyl (DPPH) scavenging activity were performed for the PJ and the PAE. The in vivo study was carried out using 70 Wistar rats that were divided into seven groups, each consisting of 10 rats. All groups were treated orally for 6 weeks as follows: Control (C), positive controls (CO: 1.25 mL/kg body weight [BW]; PJ: 4 mL/kg BW; and PAE: 400 mg/kg BW), Tol (550 mg/kg BW), and a mixture each of PJ-Tol and PAE-Tol. At the end of the 45th day of study, the hepatic biochemical markers (transaminases, alkaline phosphatase, total bilirubin, albumin, and total proteins) were auto-analyzed, as well as histology and oxidative stress markers of the liver were evaluated.
Results: The phytochemical analysis revealed that the DPPH scavenging activity and the total phenolic, flavonoid, and tannin contents were higher in the pomegranate peel extract versus the juice. The results also showed that Tol significantly increased liver enzyme activities and total bilirubin levels, whereas albumin and total proteins were significantly decreased. Similarly, Tol provoked a significant increase in hepatic malondialdehyde levels, with a decrease in glutathione content and glutathione peroxidase activity. The biochemical changes agreed with the hepatic histological alterations. A significant improvement in all parameters was observed in the PAE-Tol group compared with the PJ-Tol group.
Conclusion: Exposure to Tol altered the hepatic antioxidant and biochemical parameters and histological profile of the rats, and PAE was more powerful than PJ in reducing Tol liver injuries through its antioxidant activity. |
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| issn | 0972-8988 2231-0916 |
| language | English |
| last_indexed | 2024-12-13T03:21:10Z |
| publishDate | 2022-02-01 |
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| spelling | doaj.art-bfbc5304aaa9465e8b364e76275c05d82022-12-22T00:01:21ZengVeterinary WorldVeterinary World0972-89882231-09162022-02-0115237438210.14202/vetworld.2022.374-382Evaluation of the in vitro and in vivo antioxidant potential of Punica granatum L. against toluene-induced liver injuries in ratsFatma Zohra Arkoub0https://orcid.org/0000-0003-1646-6633Leila Hamdi1https://orcid.org/0000-0002-4775-6070Labiba Kahalerras2https://orcid.org/0000-0001-7981-5314Meriem Hamoudi3https://orcid.org/0000-0001-6659-3943Kamel Khelili4https://orcid.org/0000-0001-7160-5451Department of Biology, Laboratory of Animal Ecophysiology, Faculty of Sciences, University Badji Mokhtar-Annaba, Annaba 23000, Algeria.Department of Biology, Laboratory of Animal Ecophysiology, Faculty of Sciences, University Badji Mokhtar-Annaba, Annaba 23000, Algeria.Department of Biology, Laboratory of Animal Ecophysiology, Faculty of Sciences, University Badji Mokhtar-Annaba, Annaba 23000, Algeria.Department of Biology, Laboratory of Phytotherapy Applied to Chronic Diseases, Faculty of Natural and Life Sciences, University Ferhat Abbas Setif 1, Setif 19000, Algeria.Department of Biology, Laboratory of Animal Ecophysiology, Faculty of Sciences, University Badji Mokhtar-Annaba, Annaba 23000, Algeria.Background and Aim: Humans are more frequently exposed to chemicals in daily life by inhalation of indoor and outdoor air. However, abusers and workers are the most exposed to those chemicals and their health risks particularly, liver diseases. The present study investigated the protective effects of pomegranate juice (PJ) (Punica granatum) and pomegranate peel aqueous extract (PAE) supplementation against toluene (Tol)-induced hepatotoxicity in Wistar rats. Materials and Methods: A phytochemical analysis and assessment of the 2,2-diphenyl-1-picrylhydrazyl (DPPH) scavenging activity were performed for the PJ and the PAE. The in vivo study was carried out using 70 Wistar rats that were divided into seven groups, each consisting of 10 rats. All groups were treated orally for 6 weeks as follows: Control (C), positive controls (CO: 1.25 mL/kg body weight [BW]; PJ: 4 mL/kg BW; and PAE: 400 mg/kg BW), Tol (550 mg/kg BW), and a mixture each of PJ-Tol and PAE-Tol. At the end of the 45th day of study, the hepatic biochemical markers (transaminases, alkaline phosphatase, total bilirubin, albumin, and total proteins) were auto-analyzed, as well as histology and oxidative stress markers of the liver were evaluated. Results: The phytochemical analysis revealed that the DPPH scavenging activity and the total phenolic, flavonoid, and tannin contents were higher in the pomegranate peel extract versus the juice. The results also showed that Tol significantly increased liver enzyme activities and total bilirubin levels, whereas albumin and total proteins were significantly decreased. Similarly, Tol provoked a significant increase in hepatic malondialdehyde levels, with a decrease in glutathione content and glutathione peroxidase activity. The biochemical changes agreed with the hepatic histological alterations. A significant improvement in all parameters was observed in the PAE-Tol group compared with the PJ-Tol group. Conclusion: Exposure to Tol altered the hepatic antioxidant and biochemical parameters and histological profile of the rats, and PAE was more powerful than PJ in reducing Tol liver injuries through its antioxidant activity.http://www.veterinaryworld.org/Vol.15/February-2022/18.pdfantioxidant activityliver injuriesoxidative stresspunica granatumtoluene |
| spellingShingle | Fatma Zohra Arkoub Leila Hamdi Labiba Kahalerras Meriem Hamoudi Kamel Khelili Evaluation of the in vitro and in vivo antioxidant potential of Punica granatum L. against toluene-induced liver injuries in rats Veterinary World antioxidant activity liver injuries oxidative stress punica granatum toluene |
| title | Evaluation of the in vitro and in vivo antioxidant potential of Punica granatum L. against toluene-induced liver injuries in rats |
| title_full | Evaluation of the in vitro and in vivo antioxidant potential of Punica granatum L. against toluene-induced liver injuries in rats |
| title_fullStr | Evaluation of the in vitro and in vivo antioxidant potential of Punica granatum L. against toluene-induced liver injuries in rats |
| title_full_unstemmed | Evaluation of the in vitro and in vivo antioxidant potential of Punica granatum L. against toluene-induced liver injuries in rats |
| title_short | Evaluation of the in vitro and in vivo antioxidant potential of Punica granatum L. against toluene-induced liver injuries in rats |
| title_sort | evaluation of the in vitro and in vivo antioxidant potential of punica granatum l against toluene induced liver injuries in rats |
| topic | antioxidant activity liver injuries oxidative stress punica granatum toluene |
| url | http://www.veterinaryworld.org/Vol.15/February-2022/18.pdf |
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