Potential of New Bacterial Strains for a Multiproduct Bioprocess Application: A Case Study Using Isolates of Lactic Acid Bacteria from Pineapple Silage of Costa Rican Agro-Industrial Residues
Lactic acid bacteria (LAB) with potential for the development of multi-product processes are necessary for the valorization of side streams obtained during the biotechnological production of lactic acid (LA). In this study, 14 LAB strains isolated from pineapple agro-industrial residues in Costa Ric...
| Main Authors: | , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
MDPI AG
2022-07-01
|
| Series: | Fermentation |
| Subjects: | |
| Online Access: | https://www.mdpi.com/2311-5637/8/8/361 |
| _version_ | 1797410378893754368 |
|---|---|
| author | Jéssica Montero-Zamora María Daniela Rojas-Vargas Natalia Barboza José Pablo López-Gómez José Aníbal Mora-Villalobos Mauricio Redondo-Solano |
| author_facet | Jéssica Montero-Zamora María Daniela Rojas-Vargas Natalia Barboza José Pablo López-Gómez José Aníbal Mora-Villalobos Mauricio Redondo-Solano |
| author_sort | Jéssica Montero-Zamora |
| collection | DOAJ |
| description | Lactic acid bacteria (LAB) with potential for the development of multi-product processes are necessary for the valorization of side streams obtained during the biotechnological production of lactic acid (LA). In this study, 14 LAB strains isolated from pineapple agro-industrial residues in Costa Rica were cultivated in microplates, and the six strains with the highest growth were selected for fermentation in microbioreactors to evaluate the production of LA and acetic acid, and the consumption of glucose. <i>Lacticaseibacillus paracasei</i> 6710 and <i>L. paracasei</i> 6714 presented the highest OD<sub>600</sub> values (1.600 and 1.602, respectively); however, the highest LA (in g/L) production was observed in <i>L. paracasei</i> 6714 (14.50 ± 0.20) and 6712 (14.67 ± 0.42). <i>L. paracasei</i> 6714 was selected for bioreactor fermentation and reached a maximum OD<sub>600</sub> of 6.3062 ± 0.141, with a LA yield of 84.9% and a productivity of 1.06 g L<sup>−1</sup> h<sup>−1</sup> after 21 h of fermentation. Finally, lipoteichoic acid (LTA) detection from biomass was performed and the antimicrobial activity of the compounds present in the supernatant was studied. LTA was detected from <i>L. paracasei</i> 6714 biomass, and its supernatant caused significant inhibition of foodborne surrogate microorganisms. LAB isolated from pineapple silage have biotechnological potential for multiproduct processes. |
| first_indexed | 2024-03-09T04:29:05Z |
| format | Article |
| id | doaj.art-bfbfdd122afd4966842837381b110fe9 |
| institution | Directory Open Access Journal |
| issn | 2311-5637 |
| language | English |
| last_indexed | 2024-03-09T04:29:05Z |
| publishDate | 2022-07-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Fermentation |
| spelling | doaj.art-bfbfdd122afd4966842837381b110fe92023-12-03T13:37:52ZengMDPI AGFermentation2311-56372022-07-018836110.3390/fermentation8080361Potential of New Bacterial Strains for a Multiproduct Bioprocess Application: A Case Study Using Isolates of Lactic Acid Bacteria from Pineapple Silage of Costa Rican Agro-Industrial ResiduesJéssica Montero-Zamora0María Daniela Rojas-Vargas1Natalia Barboza2José Pablo López-Gómez3José Aníbal Mora-Villalobos4Mauricio Redondo-Solano5National Center for Biotechnological Innovations of Costa Rica (CENIBiot), San Jose 1174-1200, Costa RicaTropical Disease Investigation Center (CIET), Department of Microbiology and Immunology, Faculty of Microbiology, University of Costa Rica, San Jose 11501-2060, Costa RicaFood Technology Department, University of Costa Rica, San Jose 11501-2060, Costa RicaNational Center for Biotechnological Innovations of Costa Rica (CENIBiot), San Jose 1174-1200, Costa RicaNational Center for Biotechnological Innovations of Costa Rica (CENIBiot), San Jose 1174-1200, Costa RicaTropical Disease Investigation Center (CIET), Department of Microbiology and Immunology, Faculty of Microbiology, University of Costa Rica, San Jose 11501-2060, Costa RicaLactic acid bacteria (LAB) with potential for the development of multi-product processes are necessary for the valorization of side streams obtained during the biotechnological production of lactic acid (LA). In this study, 14 LAB strains isolated from pineapple agro-industrial residues in Costa Rica were cultivated in microplates, and the six strains with the highest growth were selected for fermentation in microbioreactors to evaluate the production of LA and acetic acid, and the consumption of glucose. <i>Lacticaseibacillus paracasei</i> 6710 and <i>L. paracasei</i> 6714 presented the highest OD<sub>600</sub> values (1.600 and 1.602, respectively); however, the highest LA (in g/L) production was observed in <i>L. paracasei</i> 6714 (14.50 ± 0.20) and 6712 (14.67 ± 0.42). <i>L. paracasei</i> 6714 was selected for bioreactor fermentation and reached a maximum OD<sub>600</sub> of 6.3062 ± 0.141, with a LA yield of 84.9% and a productivity of 1.06 g L<sup>−1</sup> h<sup>−1</sup> after 21 h of fermentation. Finally, lipoteichoic acid (LTA) detection from biomass was performed and the antimicrobial activity of the compounds present in the supernatant was studied. LTA was detected from <i>L. paracasei</i> 6714 biomass, and its supernatant caused significant inhibition of foodborne surrogate microorganisms. LAB isolated from pineapple silage have biotechnological potential for multiproduct processes.https://www.mdpi.com/2311-5637/8/8/361lactic acidlipoteichoic acidantimicrobial compoundsfermentationcircular economybioproducts valorization |
| spellingShingle | Jéssica Montero-Zamora María Daniela Rojas-Vargas Natalia Barboza José Pablo López-Gómez José Aníbal Mora-Villalobos Mauricio Redondo-Solano Potential of New Bacterial Strains for a Multiproduct Bioprocess Application: A Case Study Using Isolates of Lactic Acid Bacteria from Pineapple Silage of Costa Rican Agro-Industrial Residues Fermentation lactic acid lipoteichoic acid antimicrobial compounds fermentation circular economy bioproducts valorization |
| title | Potential of New Bacterial Strains for a Multiproduct Bioprocess Application: A Case Study Using Isolates of Lactic Acid Bacteria from Pineapple Silage of Costa Rican Agro-Industrial Residues |
| title_full | Potential of New Bacterial Strains for a Multiproduct Bioprocess Application: A Case Study Using Isolates of Lactic Acid Bacteria from Pineapple Silage of Costa Rican Agro-Industrial Residues |
| title_fullStr | Potential of New Bacterial Strains for a Multiproduct Bioprocess Application: A Case Study Using Isolates of Lactic Acid Bacteria from Pineapple Silage of Costa Rican Agro-Industrial Residues |
| title_full_unstemmed | Potential of New Bacterial Strains for a Multiproduct Bioprocess Application: A Case Study Using Isolates of Lactic Acid Bacteria from Pineapple Silage of Costa Rican Agro-Industrial Residues |
| title_short | Potential of New Bacterial Strains for a Multiproduct Bioprocess Application: A Case Study Using Isolates of Lactic Acid Bacteria from Pineapple Silage of Costa Rican Agro-Industrial Residues |
| title_sort | potential of new bacterial strains for a multiproduct bioprocess application a case study using isolates of lactic acid bacteria from pineapple silage of costa rican agro industrial residues |
| topic | lactic acid lipoteichoic acid antimicrobial compounds fermentation circular economy bioproducts valorization |
| url | https://www.mdpi.com/2311-5637/8/8/361 |
| work_keys_str_mv | AT jessicamonterozamora potentialofnewbacterialstrainsforamultiproductbioprocessapplicationacasestudyusingisolatesoflacticacidbacteriafrompineapplesilageofcostaricanagroindustrialresidues AT mariadanielarojasvargas potentialofnewbacterialstrainsforamultiproductbioprocessapplicationacasestudyusingisolatesoflacticacidbacteriafrompineapplesilageofcostaricanagroindustrialresidues AT nataliabarboza potentialofnewbacterialstrainsforamultiproductbioprocessapplicationacasestudyusingisolatesoflacticacidbacteriafrompineapplesilageofcostaricanagroindustrialresidues AT josepablolopezgomez potentialofnewbacterialstrainsforamultiproductbioprocessapplicationacasestudyusingisolatesoflacticacidbacteriafrompineapplesilageofcostaricanagroindustrialresidues AT joseanibalmoravillalobos potentialofnewbacterialstrainsforamultiproductbioprocessapplicationacasestudyusingisolatesoflacticacidbacteriafrompineapplesilageofcostaricanagroindustrialresidues AT mauricioredondosolano potentialofnewbacterialstrainsforamultiproductbioprocessapplicationacasestudyusingisolatesoflacticacidbacteriafrompineapplesilageofcostaricanagroindustrialresidues |