Lignocellulose Degrading <i>Weizmannia coagulans</i> Capable of Enantiomeric L-Lactic Acid Production via Consolidated Bioprocessing
Second-generation lactic acid production requires the development of sustainable and economically feasible processes and renewable lignocellulose biomass as a starting raw material. <i>Weizmannia coagulans</i> MA42 was isolated from a soil sample in Chiang Mai province, Thailand and show...
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MDPI AG
2023-08-01
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| Series: | Fermentation |
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| author | Punnita Pamueangmun Aliyu Dantani Abdullahi Md. Humayun Kabir Kridsada Unban Apinun Kanpiengjai Joachim Venus Kalidas Shetty Chalermpong Saenjum Chartchai Khanongnuch |
| author_facet | Punnita Pamueangmun Aliyu Dantani Abdullahi Md. Humayun Kabir Kridsada Unban Apinun Kanpiengjai Joachim Venus Kalidas Shetty Chalermpong Saenjum Chartchai Khanongnuch |
| author_sort | Punnita Pamueangmun |
| collection | DOAJ |
| description | Second-generation lactic acid production requires the development of sustainable and economically feasible processes and renewable lignocellulose biomass as a starting raw material. <i>Weizmannia coagulans</i> MA42 was isolated from a soil sample in Chiang Mai province, Thailand and showed the highest production of L-lactic acid and lignocellulolytic enzymes (cellulase, β-mannanase, xylanase, β-glucosidase, β-mannosidase, and β-xylosidase) compared to other isolates. <i>Weizmannia coagulans</i> MA42 was able to grow, secrete lignocellulolytic enzymes, and directly produce L-lactic acid in the medium containing various lignocellulosic feedstocks as the sole carbon source. Moreover, L-lactic acid production efficiency was improved after the substrates were pretreated with diluted sulfuric acid and diluted sodium hydroxide. The highest L-lactic acid production efficiency of 553.4 ± 2.9, 325.4 ± 4.1, 326.6 ± 4.4, 528.0 ± 7.2, and 547.0 ± 2.2 mg/g total available carbohydrate was obtained from respective pretreated substrates including sugarcane bagasse, sugarcane trash, corn stover, rice straw, and water hyacinth. It is suggested that structural complexity of the lignocellulosic materials and properties of lignocellulolytic enzymes are the key factors of consolidated bioprocessing (CBP) of lignocellulosic feedstocks to lactic acid. In addition, the results of this study indicated that <i>W. coagulans</i> MA42 is a potent bacterial candidate for CBP of a variety of lignocellulosic feedstocks to L-lactic acid production; however, further bioprocess development and genetic engineering technique would provide higher lactic acid production efficiency, and this would lead to sustainable lactic acid production from lignocellulosic feedstocks. |
| first_indexed | 2024-03-10T23:57:39Z |
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| id | doaj.art-bfc38ed20e274922b28253a107d8889e |
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| issn | 2311-5637 |
| language | English |
| last_indexed | 2024-03-10T23:57:39Z |
| publishDate | 2023-08-01 |
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| series | Fermentation |
| spelling | doaj.art-bfc38ed20e274922b28253a107d8889e2023-11-19T01:02:27ZengMDPI AGFermentation2311-56372023-08-019876110.3390/fermentation9080761Lignocellulose Degrading <i>Weizmannia coagulans</i> Capable of Enantiomeric L-Lactic Acid Production via Consolidated BioprocessingPunnita Pamueangmun0Aliyu Dantani Abdullahi1Md. Humayun Kabir2Kridsada Unban3Apinun Kanpiengjai4Joachim Venus5Kalidas Shetty6Chalermpong Saenjum7Chartchai Khanongnuch8Division of Biotechnology, School of Agro-Industry, Faculty of Agro-Industry, Chiang Mai University, Chiang Mai 50100, ThailandDivision of Biotechnology, School of Agro-Industry, Faculty of Agro-Industry, Chiang Mai University, Chiang Mai 50100, ThailandDivision of Biotechnology, School of Agro-Industry, Faculty of Agro-Industry, Chiang Mai University, Chiang Mai 50100, ThailandDivision of Food Science and Technology, School of Agro-Industry, Faculty of Agro-Industry, Chiang Mai University, Chiang Mai 50100, ThailandResearch Center for Multidisciplinary Approaches to Miang, Chiang Mai University, Chiang Mai 50200, ThailandLeibniz Institute for Agricultural Engineering and Bioeconomy, Department of Microbiome Biotechnology, Max-Eyth-Allee 100, 14469 Potsdam, GermanyGlobal Institute of Food Security and International Agriculture (GIFSIA), Department of Plant Sciences, North Dakota State University, Fargo, ND 58108, USACluster of Excellence on Biodiversity-Based Economic and Society (B.BES-CMU), Chiang Mai University, Chiang Mai 50200, ThailandDivision of Biotechnology, School of Agro-Industry, Faculty of Agro-Industry, Chiang Mai University, Chiang Mai 50100, ThailandSecond-generation lactic acid production requires the development of sustainable and economically feasible processes and renewable lignocellulose biomass as a starting raw material. <i>Weizmannia coagulans</i> MA42 was isolated from a soil sample in Chiang Mai province, Thailand and showed the highest production of L-lactic acid and lignocellulolytic enzymes (cellulase, β-mannanase, xylanase, β-glucosidase, β-mannosidase, and β-xylosidase) compared to other isolates. <i>Weizmannia coagulans</i> MA42 was able to grow, secrete lignocellulolytic enzymes, and directly produce L-lactic acid in the medium containing various lignocellulosic feedstocks as the sole carbon source. Moreover, L-lactic acid production efficiency was improved after the substrates were pretreated with diluted sulfuric acid and diluted sodium hydroxide. The highest L-lactic acid production efficiency of 553.4 ± 2.9, 325.4 ± 4.1, 326.6 ± 4.4, 528.0 ± 7.2, and 547.0 ± 2.2 mg/g total available carbohydrate was obtained from respective pretreated substrates including sugarcane bagasse, sugarcane trash, corn stover, rice straw, and water hyacinth. It is suggested that structural complexity of the lignocellulosic materials and properties of lignocellulolytic enzymes are the key factors of consolidated bioprocessing (CBP) of lignocellulosic feedstocks to lactic acid. In addition, the results of this study indicated that <i>W. coagulans</i> MA42 is a potent bacterial candidate for CBP of a variety of lignocellulosic feedstocks to L-lactic acid production; however, further bioprocess development and genetic engineering technique would provide higher lactic acid production efficiency, and this would lead to sustainable lactic acid production from lignocellulosic feedstocks.https://www.mdpi.com/2311-5637/9/8/761lignocellulose<i>Bacillus coagulans</i><i>Weizmannia coagulans</i>lignocellulolytic enzymesconsolidated bioprocessing (CBP)L-lactic acid |
| spellingShingle | Punnita Pamueangmun Aliyu Dantani Abdullahi Md. Humayun Kabir Kridsada Unban Apinun Kanpiengjai Joachim Venus Kalidas Shetty Chalermpong Saenjum Chartchai Khanongnuch Lignocellulose Degrading <i>Weizmannia coagulans</i> Capable of Enantiomeric L-Lactic Acid Production via Consolidated Bioprocessing Fermentation lignocellulose <i>Bacillus coagulans</i> <i>Weizmannia coagulans</i> lignocellulolytic enzymes consolidated bioprocessing (CBP) L-lactic acid |
| title | Lignocellulose Degrading <i>Weizmannia coagulans</i> Capable of Enantiomeric L-Lactic Acid Production via Consolidated Bioprocessing |
| title_full | Lignocellulose Degrading <i>Weizmannia coagulans</i> Capable of Enantiomeric L-Lactic Acid Production via Consolidated Bioprocessing |
| title_fullStr | Lignocellulose Degrading <i>Weizmannia coagulans</i> Capable of Enantiomeric L-Lactic Acid Production via Consolidated Bioprocessing |
| title_full_unstemmed | Lignocellulose Degrading <i>Weizmannia coagulans</i> Capable of Enantiomeric L-Lactic Acid Production via Consolidated Bioprocessing |
| title_short | Lignocellulose Degrading <i>Weizmannia coagulans</i> Capable of Enantiomeric L-Lactic Acid Production via Consolidated Bioprocessing |
| title_sort | lignocellulose degrading i weizmannia coagulans i capable of enantiomeric l lactic acid production via consolidated bioprocessing |
| topic | lignocellulose <i>Bacillus coagulans</i> <i>Weizmannia coagulans</i> lignocellulolytic enzymes consolidated bioprocessing (CBP) L-lactic acid |
| url | https://www.mdpi.com/2311-5637/9/8/761 |
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