Quantification of FEND and ITUDI Anti-fungal Lipopeptide Gene Expression in Bacillus megaterium using RT-qPCR
Phytopathogenic diseases are a major concern in modern agriculture, and for decades, pesticides have been used to prevent potential damage. Bacillus megaterium is proposed as a biological controlling agent, and gene expression of the lipopeptide genes FEND and ITUDI was assessed using RT-qPCR. Inhib...
| Main Authors: | , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Journal of Pure and Applied Microbiology
2020-12-01
|
| Series: | Journal of Pure and Applied Microbiology |
| Subjects: | |
| Online Access: | https://microbiologyjournal.org/quantification-of-fend-and-itudi-anti-fungal-lipopeptide-gene-expression-in-bacillus-megaterium-using-rt-qpcr/ |
| _version_ | 1819137227466211328 |
|---|---|
| author | Viviana Pamela Chiluisa-Utreras Katherine Alejandra Medrano Jara Andrés Sebastián Cadena Paredes Ramiro Daniel Acurio |
| author_facet | Viviana Pamela Chiluisa-Utreras Katherine Alejandra Medrano Jara Andrés Sebastián Cadena Paredes Ramiro Daniel Acurio |
| author_sort | Viviana Pamela Chiluisa-Utreras |
| collection | DOAJ |
| description | Phytopathogenic diseases are a major concern in modern agriculture, and for decades, pesticides have been used to prevent potential damage. Bacillus megaterium is proposed as a biological controlling agent, and gene expression of the lipopeptide genes FEND and ITUDI was assessed using RT-qPCR. Inhibition effects of B. megaterium on Alternaria sp. and Botrytis sp. were examined over a period of nine days, which confirmed the potential use of this bacterium to counteract these two pathogens. In addition, expression of FEND and ITUDI genes was assessed over nine days in the aforementioned dual cultures and inhibition tests. FEND expression in B. megaterium increased 20.16-fold in response to Alternaria sp., and ITUDI expression increased 3.20-fold in response to Botrytis sp. on day five of incubation. These results were corroborated by gene expression data obtained from B. megaterium during fermentation, where FEND and ITUDI gene expression increased 95.14- and 18.70-fold, respectively. In conclusion, B. megaterium can increase lipopeptide synthesis when exposed to these particular phytopathogens and can significantly increase the respective expression during fermentation. |
| first_indexed | 2024-12-22T10:47:31Z |
| format | Article |
| id | doaj.art-bfc5591ea02140ff983016d1adedf40c |
| institution | Directory Open Access Journal |
| issn | 0973-7510 2581-690X |
| language | English |
| last_indexed | 2024-12-22T10:47:31Z |
| publishDate | 2020-12-01 |
| publisher | Journal of Pure and Applied Microbiology |
| record_format | Article |
| series | Journal of Pure and Applied Microbiology |
| spelling | doaj.art-bfc5591ea02140ff983016d1adedf40c2022-12-21T18:28:54ZengJournal of Pure and Applied MicrobiologyJournal of Pure and Applied Microbiology0973-75102581-690X2020-12-011442339234910.22207/JPAM.14.4.12Quantification of FEND and ITUDI Anti-fungal Lipopeptide Gene Expression in Bacillus megaterium using RT-qPCRViviana Pamela Chiluisa-Utrerashttps://orcid.org/0000-0001-8647-1791Katherine Alejandra Medrano Jarahttps://orcid.org/0000-0001-8108-9825Andrés Sebastián Cadena Paredeshttps://orcid.org/0000-0001-9985-8290Ramiro Daniel Acuriohttps://orcid.org/0000-0002-2305-4349Phytopathogenic diseases are a major concern in modern agriculture, and for decades, pesticides have been used to prevent potential damage. Bacillus megaterium is proposed as a biological controlling agent, and gene expression of the lipopeptide genes FEND and ITUDI was assessed using RT-qPCR. Inhibition effects of B. megaterium on Alternaria sp. and Botrytis sp. were examined over a period of nine days, which confirmed the potential use of this bacterium to counteract these two pathogens. In addition, expression of FEND and ITUDI genes was assessed over nine days in the aforementioned dual cultures and inhibition tests. FEND expression in B. megaterium increased 20.16-fold in response to Alternaria sp., and ITUDI expression increased 3.20-fold in response to Botrytis sp. on day five of incubation. These results were corroborated by gene expression data obtained from B. megaterium during fermentation, where FEND and ITUDI gene expression increased 95.14- and 18.70-fold, respectively. In conclusion, B. megaterium can increase lipopeptide synthesis when exposed to these particular phytopathogens and can significantly increase the respective expression during fermentation.https://microbiologyjournal.org/quantification-of-fend-and-itudi-anti-fungal-lipopeptide-gene-expression-in-bacillus-megaterium-using-rt-qpcr/gene expressionantagonismantimicrobial genesplant pathogens |
| spellingShingle | Viviana Pamela Chiluisa-Utreras Katherine Alejandra Medrano Jara Andrés Sebastián Cadena Paredes Ramiro Daniel Acurio Quantification of FEND and ITUDI Anti-fungal Lipopeptide Gene Expression in Bacillus megaterium using RT-qPCR Journal of Pure and Applied Microbiology gene expression antagonism antimicrobial genes plant pathogens |
| title | Quantification of FEND and ITUDI Anti-fungal Lipopeptide Gene Expression in Bacillus megaterium using RT-qPCR |
| title_full | Quantification of FEND and ITUDI Anti-fungal Lipopeptide Gene Expression in Bacillus megaterium using RT-qPCR |
| title_fullStr | Quantification of FEND and ITUDI Anti-fungal Lipopeptide Gene Expression in Bacillus megaterium using RT-qPCR |
| title_full_unstemmed | Quantification of FEND and ITUDI Anti-fungal Lipopeptide Gene Expression in Bacillus megaterium using RT-qPCR |
| title_short | Quantification of FEND and ITUDI Anti-fungal Lipopeptide Gene Expression in Bacillus megaterium using RT-qPCR |
| title_sort | quantification of fend and itudi anti fungal lipopeptide gene expression in bacillus megaterium using rt qpcr |
| topic | gene expression antagonism antimicrobial genes plant pathogens |
| url | https://microbiologyjournal.org/quantification-of-fend-and-itudi-anti-fungal-lipopeptide-gene-expression-in-bacillus-megaterium-using-rt-qpcr/ |
| work_keys_str_mv | AT vivianapamelachiluisautreras quantificationoffendanditudiantifungallipopeptidegeneexpressioninbacillusmegateriumusingrtqpcr AT katherinealejandramedranojara quantificationoffendanditudiantifungallipopeptidegeneexpressioninbacillusmegateriumusingrtqpcr AT andressebastiancadenaparedes quantificationoffendanditudiantifungallipopeptidegeneexpressioninbacillusmegateriumusingrtqpcr AT ramirodanielacurio quantificationoffendanditudiantifungallipopeptidegeneexpressioninbacillusmegateriumusingrtqpcr |