A Multidirectional Perspective for Novel Functional Products: In vitro Pharmacological Activities and In silico Studies on Ononis natrix subsp. hispanica
The genus Ononis has important value as traditional drugs and foods. In the present work, we aimed to assess the chemical profiles and biological effects of Ononis natrix subsp. hispanica extracts (ethyl acetate, methanol, and water). For chemical profile, total and individual phenolic components we...
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| Format: | Article |
| Language: | English |
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Frontiers Media S.A.
2017-09-01
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| Series: | Frontiers in Pharmacology |
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| Online Access: | http://journal.frontiersin.org/article/10.3389/fphar.2017.00600/full |
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| author | Serife Yerlikaya Gokhan Zengin Adriano Mollica Mehmet C. Baloglu Yasemin Celik Altunoglu Abdurrahman Aktumsek |
| author_facet | Serife Yerlikaya Gokhan Zengin Adriano Mollica Mehmet C. Baloglu Yasemin Celik Altunoglu Abdurrahman Aktumsek |
| author_sort | Serife Yerlikaya |
| collection | DOAJ |
| description | The genus Ononis has important value as traditional drugs and foods. In the present work, we aimed to assess the chemical profiles and biological effects of Ononis natrix subsp. hispanica extracts (ethyl acetate, methanol, and water). For chemical profile, total and individual phenolic components were detected. For biological effects, antioxidant (DPPH, ABTS, CUPRAC, FRAP, phosphomolybdenum, and metal chelating assays), enzyme inhibitory (against cholinesterase, tyrosinase, α-amylase and α-glucosidase), antimicrobial, DNA protection and cytotoxic abilities were tested. The predominant phenolics were apigenin, luteolin, and quercetin in the tested extracts. Generally, the ethyl acetate and methanol extracts were noted as the most active in the antioxidant and enzyme inhibitory assays. Water extract with different concentrations indicated high level of DNA protection activity. Methanol and ethyl acetate extracts showed antibacterial effect against to Staphylococcus aureus and Staphylococcus epidermidis strains. The cytotoxic effects of O. natrix subsp. hispanica extracts on the survival of HeLa and PC3 cells were determined by MTT cell viability assay. Water and methanol extracts caused initiation of apoptosis for PC3 cell line. Furthermore, molecular docking was performed to better understand interactions between dominant phenolic compounds and selected enzymes. Our results clearly indicate that O. natrix subsp. hispanica could be considered a potential candidate for designing novel pharmaceuticals, cosmeceuticals and nutraceuticals. |
| first_indexed | 2024-04-12T14:08:58Z |
| format | Article |
| id | doaj.art-bfcf87cd659a4c76a8ea87f635d464c0 |
| institution | Directory Open Access Journal |
| issn | 1663-9812 |
| language | English |
| last_indexed | 2024-04-12T14:08:58Z |
| publishDate | 2017-09-01 |
| publisher | Frontiers Media S.A. |
| record_format | Article |
| series | Frontiers in Pharmacology |
| spelling | doaj.art-bfcf87cd659a4c76a8ea87f635d464c02022-12-22T03:29:58ZengFrontiers Media S.A.Frontiers in Pharmacology1663-98122017-09-01810.3389/fphar.2017.00600293704A Multidirectional Perspective for Novel Functional Products: In vitro Pharmacological Activities and In silico Studies on Ononis natrix subsp. hispanicaSerife Yerlikaya0Gokhan Zengin1Adriano Mollica2Mehmet C. Baloglu3Yasemin Celik Altunoglu4Abdurrahman Aktumsek5Department of Genetics and Bioengineering, Faculty of Engineering and Architecture, Kastamonu UniversityKastamonu, TurkeyDepartment of Biology, Science Faculty, Selcuk UniversityKonya, TurkeyDepartment of Pharmacy University “G. d'Annunzio” of Chieti-PescaraChieti, ItalyDepartment of Genetics and Bioengineering, Faculty of Engineering and Architecture, Kastamonu UniversityKastamonu, TurkeyDepartment of Genetics and Bioengineering, Faculty of Engineering and Architecture, Kastamonu UniversityKastamonu, TurkeyDepartment of Biology, Science Faculty, Selcuk UniversityKonya, TurkeyThe genus Ononis has important value as traditional drugs and foods. In the present work, we aimed to assess the chemical profiles and biological effects of Ononis natrix subsp. hispanica extracts (ethyl acetate, methanol, and water). For chemical profile, total and individual phenolic components were detected. For biological effects, antioxidant (DPPH, ABTS, CUPRAC, FRAP, phosphomolybdenum, and metal chelating assays), enzyme inhibitory (against cholinesterase, tyrosinase, α-amylase and α-glucosidase), antimicrobial, DNA protection and cytotoxic abilities were tested. The predominant phenolics were apigenin, luteolin, and quercetin in the tested extracts. Generally, the ethyl acetate and methanol extracts were noted as the most active in the antioxidant and enzyme inhibitory assays. Water extract with different concentrations indicated high level of DNA protection activity. Methanol and ethyl acetate extracts showed antibacterial effect against to Staphylococcus aureus and Staphylococcus epidermidis strains. The cytotoxic effects of O. natrix subsp. hispanica extracts on the survival of HeLa and PC3 cells were determined by MTT cell viability assay. Water and methanol extracts caused initiation of apoptosis for PC3 cell line. Furthermore, molecular docking was performed to better understand interactions between dominant phenolic compounds and selected enzymes. Our results clearly indicate that O. natrix subsp. hispanica could be considered a potential candidate for designing novel pharmaceuticals, cosmeceuticals and nutraceuticals.http://journal.frontiersin.org/article/10.3389/fphar.2017.00600/fullOnonisbioactive compoundsnatural agentspharmaceuticalsmolecular docking |
| spellingShingle | Serife Yerlikaya Gokhan Zengin Adriano Mollica Mehmet C. Baloglu Yasemin Celik Altunoglu Abdurrahman Aktumsek A Multidirectional Perspective for Novel Functional Products: In vitro Pharmacological Activities and In silico Studies on Ononis natrix subsp. hispanica Frontiers in Pharmacology Ononis bioactive compounds natural agents pharmaceuticals molecular docking |
| title | A Multidirectional Perspective for Novel Functional Products: In vitro Pharmacological Activities and In silico Studies on Ononis natrix subsp. hispanica |
| title_full | A Multidirectional Perspective for Novel Functional Products: In vitro Pharmacological Activities and In silico Studies on Ononis natrix subsp. hispanica |
| title_fullStr | A Multidirectional Perspective for Novel Functional Products: In vitro Pharmacological Activities and In silico Studies on Ononis natrix subsp. hispanica |
| title_full_unstemmed | A Multidirectional Perspective for Novel Functional Products: In vitro Pharmacological Activities and In silico Studies on Ononis natrix subsp. hispanica |
| title_short | A Multidirectional Perspective for Novel Functional Products: In vitro Pharmacological Activities and In silico Studies on Ononis natrix subsp. hispanica |
| title_sort | multidirectional perspective for novel functional products in vitro pharmacological activities and in silico studies on ononis natrix subsp hispanica |
| topic | Ononis bioactive compounds natural agents pharmaceuticals molecular docking |
| url | http://journal.frontiersin.org/article/10.3389/fphar.2017.00600/full |
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