Oligopeptide of RDPEER from watermelon seeds prevents heat stress-induced liver injury by suppressing oxidative stress and inflammation responses
In this paper, the effect and potential mechanism of supplementary RDPEER (P1, 801 Da), an antioxidant oligopeptide derived from watermelon seeds, on heat stress-induced rat liver injury and HepG2 cell damage were studied. It was observed that supplementation with RDPEER attenuated heat stress-induc...
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| Format: | Article |
| Language: | English |
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Elsevier
2023-06-01
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| Series: | Journal of Functional Foods |
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| Online Access: | http://www.sciencedirect.com/science/article/pii/S1756464623001639 |
| _version_ | 1797819908822663168 |
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| author | Simeng Chen Jie Zhou Chidimma Juliet Igbokwe Yuqing Duan Meihong Cai Yuanqing He Haihui Zhang |
| author_facet | Simeng Chen Jie Zhou Chidimma Juliet Igbokwe Yuqing Duan Meihong Cai Yuanqing He Haihui Zhang |
| author_sort | Simeng Chen |
| collection | DOAJ |
| description | In this paper, the effect and potential mechanism of supplementary RDPEER (P1, 801 Da), an antioxidant oligopeptide derived from watermelon seeds, on heat stress-induced rat liver injury and HepG2 cell damage were studied. It was observed that supplementation with RDPEER attenuated heat stress-induced hepatocyte injury by increasing HepG2 cell viability, reducing ROS overproduction, improving antioxidant enzyme activity (T-SOD, GSH-Px and CAT) and decreasing MDA content. RDPEER also inhibited HPA axis hyperactivity, serum transaminase activity (AST, ALT) and inflammatory factor levels (INF-γ, IL-6 and TNF-α), activated the endogenous antioxidant pathway (Nrf2/Keap-1), and reduced inflammatory factor transcription (NFκB) and heat shock protein expression (HSP27/72/90). In conclusion, supplementation with RDPEER could enhance antioxidant capacity in vivo and in vitro, reduce the inflammatory and heat shock response to alleviate liver damage caused by heat stress. These findings may provide new insights to alleviate heat stress through nutritional interventions. |
| first_indexed | 2024-03-13T09:29:31Z |
| format | Article |
| id | doaj.art-bfd0147d754249cd8b79fed5e0978d13 |
| institution | Directory Open Access Journal |
| issn | 1756-4646 |
| language | English |
| last_indexed | 2024-03-13T09:29:31Z |
| publishDate | 2023-06-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Journal of Functional Foods |
| spelling | doaj.art-bfd0147d754249cd8b79fed5e0978d132023-05-26T04:21:12ZengElsevierJournal of Functional Foods1756-46462023-06-01105105563Oligopeptide of RDPEER from watermelon seeds prevents heat stress-induced liver injury by suppressing oxidative stress and inflammation responsesSimeng Chen0Jie Zhou1Chidimma Juliet Igbokwe2Yuqing Duan3Meihong Cai4Yuanqing He5Haihui Zhang6School of Food and Biological Engineering, Jiangsu University, Zhenjiang 212013, ChinaSchool of Food and Biological Engineering, Jiangsu University, Zhenjiang 212013, ChinaSchool of Food and Biological Engineering, Jiangsu University, Zhenjiang 212013, China; Department of Food Science and Technology, University of Nigeria Nsukka, Enugu State, NigeriaSchool of Food and Biological Engineering, Jiangsu University, Zhenjiang 212013, China; Institute of Food Physical Processing, Jiangsu University, Zhenjiang 212013, China; Nourse Nutrition Science Jiangsu Institute, Zhenjiang 212009, China; Corresponding authors at: School of Food and Biological Engineering, Jiangsu University, Zhenjiang 212013, China.School of Food and Biological Engineering, Jiangsu University, Zhenjiang 212013, ChinaSchool of Food and Biological Engineering, Jiangsu University, Zhenjiang 212013, ChinaSchool of Food and Biological Engineering, Jiangsu University, Zhenjiang 212013, China; Corresponding authors at: School of Food and Biological Engineering, Jiangsu University, Zhenjiang 212013, China.In this paper, the effect and potential mechanism of supplementary RDPEER (P1, 801 Da), an antioxidant oligopeptide derived from watermelon seeds, on heat stress-induced rat liver injury and HepG2 cell damage were studied. It was observed that supplementation with RDPEER attenuated heat stress-induced hepatocyte injury by increasing HepG2 cell viability, reducing ROS overproduction, improving antioxidant enzyme activity (T-SOD, GSH-Px and CAT) and decreasing MDA content. RDPEER also inhibited HPA axis hyperactivity, serum transaminase activity (AST, ALT) and inflammatory factor levels (INF-γ, IL-6 and TNF-α), activated the endogenous antioxidant pathway (Nrf2/Keap-1), and reduced inflammatory factor transcription (NFκB) and heat shock protein expression (HSP27/72/90). In conclusion, supplementation with RDPEER could enhance antioxidant capacity in vivo and in vitro, reduce the inflammatory and heat shock response to alleviate liver damage caused by heat stress. These findings may provide new insights to alleviate heat stress through nutritional interventions.http://www.sciencedirect.com/science/article/pii/S1756464623001639Heat stressRDPEERLiver damageOxidative stressInflammationHeat shock proteins |
| spellingShingle | Simeng Chen Jie Zhou Chidimma Juliet Igbokwe Yuqing Duan Meihong Cai Yuanqing He Haihui Zhang Oligopeptide of RDPEER from watermelon seeds prevents heat stress-induced liver injury by suppressing oxidative stress and inflammation responses Journal of Functional Foods Heat stress RDPEER Liver damage Oxidative stress Inflammation Heat shock proteins |
| title | Oligopeptide of RDPEER from watermelon seeds prevents heat stress-induced liver injury by suppressing oxidative stress and inflammation responses |
| title_full | Oligopeptide of RDPEER from watermelon seeds prevents heat stress-induced liver injury by suppressing oxidative stress and inflammation responses |
| title_fullStr | Oligopeptide of RDPEER from watermelon seeds prevents heat stress-induced liver injury by suppressing oxidative stress and inflammation responses |
| title_full_unstemmed | Oligopeptide of RDPEER from watermelon seeds prevents heat stress-induced liver injury by suppressing oxidative stress and inflammation responses |
| title_short | Oligopeptide of RDPEER from watermelon seeds prevents heat stress-induced liver injury by suppressing oxidative stress and inflammation responses |
| title_sort | oligopeptide of rdpeer from watermelon seeds prevents heat stress induced liver injury by suppressing oxidative stress and inflammation responses |
| topic | Heat stress RDPEER Liver damage Oxidative stress Inflammation Heat shock proteins |
| url | http://www.sciencedirect.com/science/article/pii/S1756464623001639 |
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