Investigation of efficacy of treatment in spinal cord injury: Erythropoietin versus methylprednisolone
Background: Investigation of the expression of platelet-derived growth factor (PDGF)-β and glial fibrillary acidic protein (GFAP) in rats with spinal cord injury as a marker of neurologic recovery between groups treated with erythropoietin (EPO) and methylprednisolone (MP). Methods: Thirty adult fem...
| Main Authors: | , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
SAGE Publishing
2017-11-01
|
| Series: | Journal of Orthopaedic Surgery |
| Online Access: | https://doi.org/10.1177/2309499017739481 |
| _version_ | 1818414826633822208 |
|---|---|
| author | Okan Ozkunt Kerim Sariyilmaz Halil Can Gemalmaz Seren Gülsen Gürgen Ulaş Yener Fatih Dikici |
| author_facet | Okan Ozkunt Kerim Sariyilmaz Halil Can Gemalmaz Seren Gülsen Gürgen Ulaş Yener Fatih Dikici |
| author_sort | Okan Ozkunt |
| collection | DOAJ |
| description | Background: Investigation of the expression of platelet-derived growth factor (PDGF)-β and glial fibrillary acidic protein (GFAP) in rats with spinal cord injury as a marker of neurologic recovery between groups treated with erythropoietin (EPO) and methylprednisolone (MP). Methods: Thirty adult female rats were randomly divided into three even groups. A laminectomy was applied to thoracic ninth vertebra and contusion injury was induced by extradural application of an aneurysm clip. Group 1 rats received one-time intrathecal administration of normal saline, group 2 rats received MP, and group 3 rats received EPO. Motor neurological function was evaluated by the Basso, Beattie, and Bresnahan (BBB) locomotor rating scale. Thirty days after the surgery, T8–10 segments of the spinal cords were extracted and the immunohistochemical assay revealed the number of PDGF-β- and GFAP-positive cells. Results: Evaluation of the last control animal showed that BBB score in the EPO group showed an increase from 1 to 12 ( p < 0.05). The immunohistochemical assay revealed that the number of PDGF-β- and GFAP-positive cells was significantly higher in EPO group ( p = 0.000) when compared to MP and control groups. After studying the effect of PDGF-β expression on the locomotor function, we determined that PDGF-β expression and locomotor function after a spinal injury has a strong relationship ( p < 0.05). Conclusion: EPO seems to better increase the expression of PDGF-β, thus produce better results in locomotor functions when compared to MP. |
| first_indexed | 2024-12-14T11:25:16Z |
| format | Article |
| id | doaj.art-bfd1c61d1cc9403e9a548b70aa60a4f3 |
| institution | Directory Open Access Journal |
| issn | 2309-4990 |
| language | English |
| last_indexed | 2024-12-14T11:25:16Z |
| publishDate | 2017-11-01 |
| publisher | SAGE Publishing |
| record_format | Article |
| series | Journal of Orthopaedic Surgery |
| spelling | doaj.art-bfd1c61d1cc9403e9a548b70aa60a4f32022-12-21T23:03:34ZengSAGE PublishingJournal of Orthopaedic Surgery2309-49902017-11-012510.1177/2309499017739481Investigation of efficacy of treatment in spinal cord injury: Erythropoietin versus methylprednisoloneOkan Ozkunt0Kerim Sariyilmaz1Halil Can Gemalmaz2Seren Gülsen Gürgen3Ulaş Yener4Fatih Dikici5 Department of Orthopedics and Traumatology, Acibadem University Atakent Hospital, Halkali/Kucukcekmece, Istanbul, Turkey Department of Orthopedics and Traumatology, Acibadem University Atakent Hospital, Halkali/Kucukcekmece, Istanbul, Turkey Department of Orthopedics and Traumatology, Acibadem University Atakent Hospital, Halkali/Kucukcekmece, Istanbul, Turkey Department of Histology and Embryology, Celal Bayar University School of Vocational Health Service, Yunusemre, Manisa, Turkey Department of Neurosurgery, Acibadem University Atakent Hospital, Halkali/Kucukcekmece, Istanbul, Turkey Department of Orthopedics and Traumatology, Acibadem University Atakent Hospital, Halkali/Kucukcekmece, Istanbul, TurkeyBackground: Investigation of the expression of platelet-derived growth factor (PDGF)-β and glial fibrillary acidic protein (GFAP) in rats with spinal cord injury as a marker of neurologic recovery between groups treated with erythropoietin (EPO) and methylprednisolone (MP). Methods: Thirty adult female rats were randomly divided into three even groups. A laminectomy was applied to thoracic ninth vertebra and contusion injury was induced by extradural application of an aneurysm clip. Group 1 rats received one-time intrathecal administration of normal saline, group 2 rats received MP, and group 3 rats received EPO. Motor neurological function was evaluated by the Basso, Beattie, and Bresnahan (BBB) locomotor rating scale. Thirty days after the surgery, T8–10 segments of the spinal cords were extracted and the immunohistochemical assay revealed the number of PDGF-β- and GFAP-positive cells. Results: Evaluation of the last control animal showed that BBB score in the EPO group showed an increase from 1 to 12 ( p < 0.05). The immunohistochemical assay revealed that the number of PDGF-β- and GFAP-positive cells was significantly higher in EPO group ( p = 0.000) when compared to MP and control groups. After studying the effect of PDGF-β expression on the locomotor function, we determined that PDGF-β expression and locomotor function after a spinal injury has a strong relationship ( p < 0.05). Conclusion: EPO seems to better increase the expression of PDGF-β, thus produce better results in locomotor functions when compared to MP.https://doi.org/10.1177/2309499017739481 |
| spellingShingle | Okan Ozkunt Kerim Sariyilmaz Halil Can Gemalmaz Seren Gülsen Gürgen Ulaş Yener Fatih Dikici Investigation of efficacy of treatment in spinal cord injury: Erythropoietin versus methylprednisolone Journal of Orthopaedic Surgery |
| title | Investigation of efficacy of treatment in spinal cord injury: Erythropoietin versus methylprednisolone |
| title_full | Investigation of efficacy of treatment in spinal cord injury: Erythropoietin versus methylprednisolone |
| title_fullStr | Investigation of efficacy of treatment in spinal cord injury: Erythropoietin versus methylprednisolone |
| title_full_unstemmed | Investigation of efficacy of treatment in spinal cord injury: Erythropoietin versus methylprednisolone |
| title_short | Investigation of efficacy of treatment in spinal cord injury: Erythropoietin versus methylprednisolone |
| title_sort | investigation of efficacy of treatment in spinal cord injury erythropoietin versus methylprednisolone |
| url | https://doi.org/10.1177/2309499017739481 |
| work_keys_str_mv | AT okanozkunt investigationofefficacyoftreatmentinspinalcordinjuryerythropoietinversusmethylprednisolone AT kerimsariyilmaz investigationofefficacyoftreatmentinspinalcordinjuryerythropoietinversusmethylprednisolone AT halilcangemalmaz investigationofefficacyoftreatmentinspinalcordinjuryerythropoietinversusmethylprednisolone AT serengulsengurgen investigationofefficacyoftreatmentinspinalcordinjuryerythropoietinversusmethylprednisolone AT ulasyener investigationofefficacyoftreatmentinspinalcordinjuryerythropoietinversusmethylprednisolone AT fatihdikici investigationofefficacyoftreatmentinspinalcordinjuryerythropoietinversusmethylprednisolone |