Transcriptome Level Analysis of Genes of Exogenous Ethylene Applied under Phosphorus Stress in Chinese Fir
Chinese fir (<i>Cunninghamia lanceolata</i> (Lamb.) Hook) is a widely grown gymnosperm in China. Phosphorus (P) is an indispensable nutrient for the growth of Chinese fir. Inorganic phosphate (Pi) deficiency exists in soils of many Chinese fir planting area regions, and the trees themsel...
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MDPI AG
2022-08-01
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| author | Shuotian Huang Lixia Zhang Tingting Cai Yuxuan Zhao Jiao Liu Pengfei Wu Xiangqing Ma Peng Shuai |
| author_facet | Shuotian Huang Lixia Zhang Tingting Cai Yuxuan Zhao Jiao Liu Pengfei Wu Xiangqing Ma Peng Shuai |
| author_sort | Shuotian Huang |
| collection | DOAJ |
| description | Chinese fir (<i>Cunninghamia lanceolata</i> (Lamb.) Hook) is a widely grown gymnosperm in China. Phosphorus (P) is an indispensable nutrient for the growth of Chinese fir. Inorganic phosphate (Pi) deficiency exists in soils of many Chinese fir planting area regions, and the trees themselves have limited efficiency in utilizing P from the soil. Ethylene is important in regulation responses to nutrient deficiencies. However, little is known about how ethylene signals participate in Pi stress in Chinese fir. A total of six different treatments were performed to reveal the transcript levels of Chinese fir under Pi, ethephon (an ethylene-releasing compound), and CoCl<sub>2</sub> (cobalt chloride, an ethylene biosynthesis inhibitor) treatments. We assembled a full-length reference transcriptome containing 22,243 unigenes as a reference for UMI RNA-seq (Digital RNA-seq). There were 586 Differentially Expressed Genes (DEGs) in the Pi starvation (NP) group, while DEGs from additional ethephon or CoCl<sub>2</sub> in NP were 708 and 292, respectively. Among the DEGs in each treatment, there were 83 TFs in these treatment groups. MYB (v-myb avian myeloblastosis viral oncogene homolog) family was the most abundant transcription factors (TFs). Three ERF (Ethylene response factor) family genes were identified when only ethylene content was imposed as a variable. Enrichment analysis indicated that the ascorbate and aldarate metabolism pathway plays a key role in resistance to Pi deficiency. This study provides insights for further elucidating the regulatory mechanism of Pi deficiency in Chinese fir. |
| first_indexed | 2024-03-09T10:06:06Z |
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| last_indexed | 2024-03-09T10:06:06Z |
| publishDate | 2022-08-01 |
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| spelling | doaj.art-bfd4b42401a1400587a7c41d8d721a462023-12-01T23:06:28ZengMDPI AGPlants2223-77472022-08-011115203610.3390/plants11152036Transcriptome Level Analysis of Genes of Exogenous Ethylene Applied under Phosphorus Stress in Chinese FirShuotian Huang0Lixia Zhang1Tingting Cai2Yuxuan Zhao3Jiao Liu4Pengfei Wu5Xiangqing Ma6Peng Shuai7College of Forestry, Fujian Agriculture and Forestry University, Fuzhou 350002, ChinaCollege of Forestry, Fujian Agriculture and Forestry University, Fuzhou 350002, ChinaCollege of Forestry, Fujian Agriculture and Forestry University, Fuzhou 350002, ChinaCollege of Forestry, Fujian Agriculture and Forestry University, Fuzhou 350002, ChinaCollege of Forestry, Fujian Agriculture and Forestry University, Fuzhou 350002, ChinaCollege of Forestry, Fujian Agriculture and Forestry University, Fuzhou 350002, ChinaCollege of Forestry, Fujian Agriculture and Forestry University, Fuzhou 350002, ChinaCollege of Forestry, Fujian Agriculture and Forestry University, Fuzhou 350002, ChinaChinese fir (<i>Cunninghamia lanceolata</i> (Lamb.) Hook) is a widely grown gymnosperm in China. Phosphorus (P) is an indispensable nutrient for the growth of Chinese fir. Inorganic phosphate (Pi) deficiency exists in soils of many Chinese fir planting area regions, and the trees themselves have limited efficiency in utilizing P from the soil. Ethylene is important in regulation responses to nutrient deficiencies. However, little is known about how ethylene signals participate in Pi stress in Chinese fir. A total of six different treatments were performed to reveal the transcript levels of Chinese fir under Pi, ethephon (an ethylene-releasing compound), and CoCl<sub>2</sub> (cobalt chloride, an ethylene biosynthesis inhibitor) treatments. We assembled a full-length reference transcriptome containing 22,243 unigenes as a reference for UMI RNA-seq (Digital RNA-seq). There were 586 Differentially Expressed Genes (DEGs) in the Pi starvation (NP) group, while DEGs from additional ethephon or CoCl<sub>2</sub> in NP were 708 and 292, respectively. Among the DEGs in each treatment, there were 83 TFs in these treatment groups. MYB (v-myb avian myeloblastosis viral oncogene homolog) family was the most abundant transcription factors (TFs). Three ERF (Ethylene response factor) family genes were identified when only ethylene content was imposed as a variable. Enrichment analysis indicated that the ascorbate and aldarate metabolism pathway plays a key role in resistance to Pi deficiency. This study provides insights for further elucidating the regulatory mechanism of Pi deficiency in Chinese fir.https://www.mdpi.com/2223-7747/11/15/2036<i>Cunninghamia lanceolata</i>UMI RNA-seqphosphorus treatmentethephontranscription factor |
| spellingShingle | Shuotian Huang Lixia Zhang Tingting Cai Yuxuan Zhao Jiao Liu Pengfei Wu Xiangqing Ma Peng Shuai Transcriptome Level Analysis of Genes of Exogenous Ethylene Applied under Phosphorus Stress in Chinese Fir Plants <i>Cunninghamia lanceolata</i> UMI RNA-seq phosphorus treatment ethephon transcription factor |
| title | Transcriptome Level Analysis of Genes of Exogenous Ethylene Applied under Phosphorus Stress in Chinese Fir |
| title_full | Transcriptome Level Analysis of Genes of Exogenous Ethylene Applied under Phosphorus Stress in Chinese Fir |
| title_fullStr | Transcriptome Level Analysis of Genes of Exogenous Ethylene Applied under Phosphorus Stress in Chinese Fir |
| title_full_unstemmed | Transcriptome Level Analysis of Genes of Exogenous Ethylene Applied under Phosphorus Stress in Chinese Fir |
| title_short | Transcriptome Level Analysis of Genes of Exogenous Ethylene Applied under Phosphorus Stress in Chinese Fir |
| title_sort | transcriptome level analysis of genes of exogenous ethylene applied under phosphorus stress in chinese fir |
| topic | <i>Cunninghamia lanceolata</i> UMI RNA-seq phosphorus treatment ethephon transcription factor |
| url | https://www.mdpi.com/2223-7747/11/15/2036 |
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