<it>Anopheles gambiae </it>heat shock protein cognate 70B impedes o'nyong-nyong virus replication
<p>Abstract</p> <p>Background</p> <p>Phylogenetic and functional analysis was conducted on an <it>Anopheles gambiae </it>gene, ENSANGG00000017398. Based on phylogenetic analysis, this gene belongs to the same lineage as Heat shock protein cognate 70-4 (Hsc70...
| Main Authors: | , , , , , |
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| Format: | Article |
| Language: | English |
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BMC
2007-07-01
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| Series: | BMC Genomics |
| Online Access: | http://www.biomedcentral.com/1471-2164/8/231 |
| _version_ | 1818146773974122496 |
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| author | Higgs Stephen Vanlandingham Dana L Tsetsarkin Konstantin A Hong Young S Sim Cheolho Collins Frank H |
| author_facet | Higgs Stephen Vanlandingham Dana L Tsetsarkin Konstantin A Hong Young S Sim Cheolho Collins Frank H |
| author_sort | Higgs Stephen |
| collection | DOAJ |
| description | <p>Abstract</p> <p>Background</p> <p>Phylogenetic and functional analysis was conducted on an <it>Anopheles gambiae </it>gene, ENSANGG00000017398. Based on phylogenetic analysis, this gene belongs to the same lineage as Heat shock protein cognate 70-4 (Hsc70-4) in <it>Drosophila</it>. Accordingly, we propose to name this gene Heat shock protein cognate 70B (HSC70B). We previously reported that expression of HSC70B and other genes including elongation factor-1α (EF-1α) and the agglutinin attachment subunit (agglutinin) were up-regulated in o'nyong-nyong virus (ONNV)-infected female <it>An. gambiae</it>. Double-stranded RNA interferences have been applied to further investigate HSC70B, EF-1α and the agglutinin functions in ONNV replication in <it>An. gambiae</it>.</p> <p>Results</p> <p>Among these three RNAi silenced genes, only dsRNAs of HSC70B (dsHSC70B) promoted ONNV replication in adult <it>An. gambiae </it>compared to the control mosquitoes that were co-injected with ONNV and dsRNA of β-galactosidase (dsβ-gal). ONNV titers from mosquitoes co-injected with dsHSC70B were about 9-fold higher at 6 days post-injection (d.p.i.) as compared to the control mosquitoes. By using ONNV tagged with enhanced green fluorescent protein (ONNV-eGFP), co-injection of ONNV-eGFP with dsHSC70B also showed approximately 2 ~ 3-fold higher GFP expression rates than the controls in the head, thorax, and abdomen of the mosquito. Furthermore, co-injection of ONNV with dsHSC70B significantly reduced the lifespan of adult mosquitoes as compared with the control, co-injection of ONNV with dsβ-gal treated mosquitoes.</p> <p>Conclusion</p> <p>These results indicate that HSC70B plays important roles in homeostasis and suppression of ONNV replication in the vector, <it>An. gambiae</it>. Biological implications of these findings are that while mosquitoes allow ONNV to replicate in them, they also check viral titers so that ONNV infection will result in no harmful effect on mosquitoes. Therefore, mosquitoes can function as vectors of ONNV transmission to humans while ONNV infection in <it>An. gambiae </it>remains asymptomatic.</p> |
| first_indexed | 2024-12-11T12:24:41Z |
| format | Article |
| id | doaj.art-bfd5203370eb45139812102ce2e7a624 |
| institution | Directory Open Access Journal |
| issn | 1471-2164 |
| language | English |
| last_indexed | 2024-12-11T12:24:41Z |
| publishDate | 2007-07-01 |
| publisher | BMC |
| record_format | Article |
| series | BMC Genomics |
| spelling | doaj.art-bfd5203370eb45139812102ce2e7a6242022-12-22T01:07:27ZengBMCBMC Genomics1471-21642007-07-018123110.1186/1471-2164-8-231<it>Anopheles gambiae </it>heat shock protein cognate 70B impedes o'nyong-nyong virus replicationHiggs StephenVanlandingham Dana LTsetsarkin Konstantin AHong Young SSim CheolhoCollins Frank H<p>Abstract</p> <p>Background</p> <p>Phylogenetic and functional analysis was conducted on an <it>Anopheles gambiae </it>gene, ENSANGG00000017398. Based on phylogenetic analysis, this gene belongs to the same lineage as Heat shock protein cognate 70-4 (Hsc70-4) in <it>Drosophila</it>. Accordingly, we propose to name this gene Heat shock protein cognate 70B (HSC70B). We previously reported that expression of HSC70B and other genes including elongation factor-1α (EF-1α) and the agglutinin attachment subunit (agglutinin) were up-regulated in o'nyong-nyong virus (ONNV)-infected female <it>An. gambiae</it>. Double-stranded RNA interferences have been applied to further investigate HSC70B, EF-1α and the agglutinin functions in ONNV replication in <it>An. gambiae</it>.</p> <p>Results</p> <p>Among these three RNAi silenced genes, only dsRNAs of HSC70B (dsHSC70B) promoted ONNV replication in adult <it>An. gambiae </it>compared to the control mosquitoes that were co-injected with ONNV and dsRNA of β-galactosidase (dsβ-gal). ONNV titers from mosquitoes co-injected with dsHSC70B were about 9-fold higher at 6 days post-injection (d.p.i.) as compared to the control mosquitoes. By using ONNV tagged with enhanced green fluorescent protein (ONNV-eGFP), co-injection of ONNV-eGFP with dsHSC70B also showed approximately 2 ~ 3-fold higher GFP expression rates than the controls in the head, thorax, and abdomen of the mosquito. Furthermore, co-injection of ONNV with dsHSC70B significantly reduced the lifespan of adult mosquitoes as compared with the control, co-injection of ONNV with dsβ-gal treated mosquitoes.</p> <p>Conclusion</p> <p>These results indicate that HSC70B plays important roles in homeostasis and suppression of ONNV replication in the vector, <it>An. gambiae</it>. Biological implications of these findings are that while mosquitoes allow ONNV to replicate in them, they also check viral titers so that ONNV infection will result in no harmful effect on mosquitoes. Therefore, mosquitoes can function as vectors of ONNV transmission to humans while ONNV infection in <it>An. gambiae </it>remains asymptomatic.</p>http://www.biomedcentral.com/1471-2164/8/231 |
| spellingShingle | Higgs Stephen Vanlandingham Dana L Tsetsarkin Konstantin A Hong Young S Sim Cheolho Collins Frank H <it>Anopheles gambiae </it>heat shock protein cognate 70B impedes o'nyong-nyong virus replication BMC Genomics |
| title | <it>Anopheles gambiae </it>heat shock protein cognate 70B impedes o'nyong-nyong virus replication |
| title_full | <it>Anopheles gambiae </it>heat shock protein cognate 70B impedes o'nyong-nyong virus replication |
| title_fullStr | <it>Anopheles gambiae </it>heat shock protein cognate 70B impedes o'nyong-nyong virus replication |
| title_full_unstemmed | <it>Anopheles gambiae </it>heat shock protein cognate 70B impedes o'nyong-nyong virus replication |
| title_short | <it>Anopheles gambiae </it>heat shock protein cognate 70B impedes o'nyong-nyong virus replication |
| title_sort | it anopheles gambiae it heat shock protein cognate 70b impedes o nyong nyong virus replication |
| url | http://www.biomedcentral.com/1471-2164/8/231 |
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