Identification of Lipids and Cytokines in Plasma and Follicular Fluid before and after Follicle-Stimulating Hormone Stimulation as Potential Markers for Follicular Maturation in Cattle
The process of follicle maturation leading to ovulation is a key milestone in female fertility. It is known that circulating lipids and cytokines play a role in the follicle’s ability to go through follicular maturation and the ovulatory processes. However, the specific mechanisms are not well under...
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| Format: | Article |
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MDPI AG
2023-10-01
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| Series: | Animals |
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| Online Access: | https://www.mdpi.com/2076-2615/13/20/3289 |
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| author | Alexandria P. Snider Renata S. Gomes Adam F. Summers Sarah C. Tenley Mohamed A. Abedal-Majed Renee M. McFee Jennifer R. Wood John S. Davis Andrea S. Cupp |
| author_facet | Alexandria P. Snider Renata S. Gomes Adam F. Summers Sarah C. Tenley Mohamed A. Abedal-Majed Renee M. McFee Jennifer R. Wood John S. Davis Andrea S. Cupp |
| author_sort | Alexandria P. Snider |
| collection | DOAJ |
| description | The process of follicle maturation leading to ovulation is a key milestone in female fertility. It is known that circulating lipids and cytokines play a role in the follicle’s ability to go through follicular maturation and the ovulatory processes. However, the specific mechanisms are not well understood. We posit that dysregulation of granulosa cells influences the ovarian environment, which tries to adapt by changing released lipids and cytokines to achieve follicular maturation. Eleven non-lactating adult females underwent estrus synchronization with two injections of PGF2α 14 days apart. Daily blood samples were collected for 28 days to monitor steroid hormone production after the second injection. To understand the potential impacts of lipids and cytokines during ovulation, a low-dose FSH stimulation (FSHLow) was performed after resynchronization of cows, and daily blood samples were collected for 14 days to monitor steroid hormone production until ovariectomies. The lipidomic analysis demonstrated increased circulating diacylglycerides and triacylglycerides during the mid-luteal phase and after FSHLow treatment. Cholesteryl esters decreased in circulation but increased in follicular fluid (FF) after FSHLow. Increased circulating concentrations of TNFα and reduced CXCL9 were observed in response to FSHLow. Therefore, specific circulating lipids and cytokines may serve as markers of normal follicle maturation. |
| first_indexed | 2024-03-10T21:29:42Z |
| format | Article |
| id | doaj.art-bfd54fcb3c244e259716a6e4d8a9842b |
| institution | Directory Open Access Journal |
| issn | 2076-2615 |
| language | English |
| last_indexed | 2024-03-10T21:29:42Z |
| publishDate | 2023-10-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Animals |
| spelling | doaj.art-bfd54fcb3c244e259716a6e4d8a9842b2023-11-19T15:25:48ZengMDPI AGAnimals2076-26152023-10-011320328910.3390/ani13203289Identification of Lipids and Cytokines in Plasma and Follicular Fluid before and after Follicle-Stimulating Hormone Stimulation as Potential Markers for Follicular Maturation in CattleAlexandria P. Snider0Renata S. Gomes1Adam F. Summers2Sarah C. Tenley3Mohamed A. Abedal-Majed4Renee M. McFee5Jennifer R. Wood6John S. Davis7Andrea S. Cupp8United States Department of Agriculture, Agricultural Research Service, U.S. Meat Animal Research Center, Clay Center, NE 68933, USADepartment of Animal Science, University of Nebraska–Lincoln, 3940 Fair Street, Lincoln, NE 68583, USAReproLogix, 2031 Indian Road, Fort Scott, KS 66071, USADepartment of Animal Science, University of Nebraska–Lincoln, 3940 Fair Street, Lincoln, NE 68583, USADepartment of Animal Production, School of Agriculture, The University of Jordan, Amman 11942, JordanSchool of Veterinary Medicine and Biomedical Sciences, University of Nebraska-Lincoln, Lincoln, NE 68583, USADepartment of Animal Science, University of Nebraska–Lincoln, 3940 Fair Street, Lincoln, NE 68583, USAOlson Center for Women’s Health, University of Nebraska Medical Center, 983255 Nebraska Medical Center, Omaha, NE 68198, USADepartment of Animal Science, University of Nebraska–Lincoln, 3940 Fair Street, Lincoln, NE 68583, USAThe process of follicle maturation leading to ovulation is a key milestone in female fertility. It is known that circulating lipids and cytokines play a role in the follicle’s ability to go through follicular maturation and the ovulatory processes. However, the specific mechanisms are not well understood. We posit that dysregulation of granulosa cells influences the ovarian environment, which tries to adapt by changing released lipids and cytokines to achieve follicular maturation. Eleven non-lactating adult females underwent estrus synchronization with two injections of PGF2α 14 days apart. Daily blood samples were collected for 28 days to monitor steroid hormone production after the second injection. To understand the potential impacts of lipids and cytokines during ovulation, a low-dose FSH stimulation (FSHLow) was performed after resynchronization of cows, and daily blood samples were collected for 14 days to monitor steroid hormone production until ovariectomies. The lipidomic analysis demonstrated increased circulating diacylglycerides and triacylglycerides during the mid-luteal phase and after FSHLow treatment. Cholesteryl esters decreased in circulation but increased in follicular fluid (FF) after FSHLow. Increased circulating concentrations of TNFα and reduced CXCL9 were observed in response to FSHLow. Therefore, specific circulating lipids and cytokines may serve as markers of normal follicle maturation.https://www.mdpi.com/2076-2615/13/20/3289low-dose FSHcowcytokineschemokineslipidsestrus cycle |
| spellingShingle | Alexandria P. Snider Renata S. Gomes Adam F. Summers Sarah C. Tenley Mohamed A. Abedal-Majed Renee M. McFee Jennifer R. Wood John S. Davis Andrea S. Cupp Identification of Lipids and Cytokines in Plasma and Follicular Fluid before and after Follicle-Stimulating Hormone Stimulation as Potential Markers for Follicular Maturation in Cattle Animals low-dose FSH cow cytokines chemokines lipids estrus cycle |
| title | Identification of Lipids and Cytokines in Plasma and Follicular Fluid before and after Follicle-Stimulating Hormone Stimulation as Potential Markers for Follicular Maturation in Cattle |
| title_full | Identification of Lipids and Cytokines in Plasma and Follicular Fluid before and after Follicle-Stimulating Hormone Stimulation as Potential Markers for Follicular Maturation in Cattle |
| title_fullStr | Identification of Lipids and Cytokines in Plasma and Follicular Fluid before and after Follicle-Stimulating Hormone Stimulation as Potential Markers for Follicular Maturation in Cattle |
| title_full_unstemmed | Identification of Lipids and Cytokines in Plasma and Follicular Fluid before and after Follicle-Stimulating Hormone Stimulation as Potential Markers for Follicular Maturation in Cattle |
| title_short | Identification of Lipids and Cytokines in Plasma and Follicular Fluid before and after Follicle-Stimulating Hormone Stimulation as Potential Markers for Follicular Maturation in Cattle |
| title_sort | identification of lipids and cytokines in plasma and follicular fluid before and after follicle stimulating hormone stimulation as potential markers for follicular maturation in cattle |
| topic | low-dose FSH cow cytokines chemokines lipids estrus cycle |
| url | https://www.mdpi.com/2076-2615/13/20/3289 |
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