Blood–Brain Barrier Biomarkers before and after Kidney Transplantation
Kidney transplantation (KT) may improve the neurological status of chronic kidney disease (CKD) patients, reflected by the altered levels of circulating BBB-specific biomarkers. This study compares the levels of neuron specific enolase (NSE), brain-derived neurotrophic factor (BDNF), neurofilament l...
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| Format: | Article |
| Language: | English |
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MDPI AG
2023-04-01
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| Series: | International Journal of Molecular Sciences |
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| Online Access: | https://www.mdpi.com/1422-0067/24/7/6628 |
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| author | Leah Hernandez Liam J. Ward Samsul Arefin Peter Barany Lars Wennberg Magnus Söderberg Stefania Bruno Vincenzo Cantaluppi Peter Stenvinkel Karolina Kublickiene |
| author_facet | Leah Hernandez Liam J. Ward Samsul Arefin Peter Barany Lars Wennberg Magnus Söderberg Stefania Bruno Vincenzo Cantaluppi Peter Stenvinkel Karolina Kublickiene |
| author_sort | Leah Hernandez |
| collection | DOAJ |
| description | Kidney transplantation (KT) may improve the neurological status of chronic kidney disease (CKD) patients, reflected by the altered levels of circulating BBB-specific biomarkers. This study compares the levels of neuron specific enolase (NSE), brain-derived neurotrophic factor (BDNF), neurofilament light chain (NfL), and circulating plasma extracellular vesicles (EVs) in kidney-failure patients before KT and at a two-year follow up. Using ELISA, NSE, BDNF, and NfL levels were measured in the plasma of 74 living-donor KT patients. Plasma EVs were isolated with ultracentrifugation, and characterized for concentration/size and surface protein expression using flow cytometry from a subset of 25 patients. Lower NSE levels, and higher BDNF and NfL were observed at the two-year follow-up compared to the baseline (<i>p</i> < 0.05). Male patients had significantly higher BDNF levels compared to those of females. BBB biomarkers correlated with the baseline lipid profile and with glucose, vitamin D, and inflammation markers after KT. BBB surrogate marker changes in the microcirculation of early vascular aging phenotype patients with calcification and/or fibrosis were observed only in NSE and BDNF. CD31+ microparticles from endothelial cells expressing inflammatory markers such as CD40 and integrins were significantly reduced after KT. KT may, thus, improve the neurological status of CKD patients, as reflected by changes in BBB-specific biomarkers. |
| first_indexed | 2024-03-11T05:35:13Z |
| format | Article |
| id | doaj.art-bfdc6db49ed8413792ca2cea174ecb13 |
| institution | Directory Open Access Journal |
| issn | 1661-6596 1422-0067 |
| language | English |
| last_indexed | 2024-03-11T05:35:13Z |
| publishDate | 2023-04-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | International Journal of Molecular Sciences |
| spelling | doaj.art-bfdc6db49ed8413792ca2cea174ecb132023-11-17T16:53:49ZengMDPI AGInternational Journal of Molecular Sciences1661-65961422-00672023-04-01247662810.3390/ijms24076628Blood–Brain Barrier Biomarkers before and after Kidney TransplantationLeah Hernandez0Liam J. Ward1Samsul Arefin2Peter Barany3Lars Wennberg4Magnus Söderberg5Stefania Bruno6Vincenzo Cantaluppi7Peter Stenvinkel8Karolina Kublickiene9Division of Renal Medicine, Department of Clinical Science, Intervention and Technology (CLINTEC), Karolinska Institutet, 171 77 Stockholm, SwedenDivision of Renal Medicine, Department of Clinical Science, Intervention and Technology (CLINTEC), Karolinska Institutet, 171 77 Stockholm, SwedenDivision of Renal Medicine, Department of Clinical Science, Intervention and Technology (CLINTEC), Karolinska Institutet, 171 77 Stockholm, SwedenDivision of Renal Medicine, Department of Clinical Science, Intervention and Technology (CLINTEC), Karolinska Institutet, 171 77 Stockholm, SwedenDepartment of Transplantation Surgery, Karolinska University Hospital, 141 86 Stockholm, SwedenDepartment of Pathology, Clinical Pharmacology and Safety Sciences, R&D AstraZeneca, 431 83 Gothenburg, SwedenDepartment of Medical Sciences, University of Torino, 10124 Torino, ItalyNephrology and Kidney Transplant Unit, Department of Translational Medicine (DIMET), University of Piemonte Orientale (UPO), “Maggiore della Carita” University Hospital, 28100 Novara, ItalyDivision of Renal Medicine, Department of Clinical Science, Intervention and Technology (CLINTEC), Karolinska Institutet, 171 77 Stockholm, SwedenDivision of Renal Medicine, Department of Clinical Science, Intervention and Technology (CLINTEC), Karolinska Institutet, 171 77 Stockholm, SwedenKidney transplantation (KT) may improve the neurological status of chronic kidney disease (CKD) patients, reflected by the altered levels of circulating BBB-specific biomarkers. This study compares the levels of neuron specific enolase (NSE), brain-derived neurotrophic factor (BDNF), neurofilament light chain (NfL), and circulating plasma extracellular vesicles (EVs) in kidney-failure patients before KT and at a two-year follow up. Using ELISA, NSE, BDNF, and NfL levels were measured in the plasma of 74 living-donor KT patients. Plasma EVs were isolated with ultracentrifugation, and characterized for concentration/size and surface protein expression using flow cytometry from a subset of 25 patients. Lower NSE levels, and higher BDNF and NfL were observed at the two-year follow-up compared to the baseline (<i>p</i> < 0.05). Male patients had significantly higher BDNF levels compared to those of females. BBB biomarkers correlated with the baseline lipid profile and with glucose, vitamin D, and inflammation markers after KT. BBB surrogate marker changes in the microcirculation of early vascular aging phenotype patients with calcification and/or fibrosis were observed only in NSE and BDNF. CD31+ microparticles from endothelial cells expressing inflammatory markers such as CD40 and integrins were significantly reduced after KT. KT may, thus, improve the neurological status of CKD patients, as reflected by changes in BBB-specific biomarkers.https://www.mdpi.com/1422-0067/24/7/6628CKDend-stage kidney failurekidney transplantationBBBNSENfL |
| spellingShingle | Leah Hernandez Liam J. Ward Samsul Arefin Peter Barany Lars Wennberg Magnus Söderberg Stefania Bruno Vincenzo Cantaluppi Peter Stenvinkel Karolina Kublickiene Blood–Brain Barrier Biomarkers before and after Kidney Transplantation International Journal of Molecular Sciences CKD end-stage kidney failure kidney transplantation BBB NSE NfL |
| title | Blood–Brain Barrier Biomarkers before and after Kidney Transplantation |
| title_full | Blood–Brain Barrier Biomarkers before and after Kidney Transplantation |
| title_fullStr | Blood–Brain Barrier Biomarkers before and after Kidney Transplantation |
| title_full_unstemmed | Blood–Brain Barrier Biomarkers before and after Kidney Transplantation |
| title_short | Blood–Brain Barrier Biomarkers before and after Kidney Transplantation |
| title_sort | blood brain barrier biomarkers before and after kidney transplantation |
| topic | CKD end-stage kidney failure kidney transplantation BBB NSE NfL |
| url | https://www.mdpi.com/1422-0067/24/7/6628 |
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