Development and characterization of a new cell line derived from European eel Anguilla anguilla kidney
A new cell line derived from the kidney of European eel, Anguilla anguilla, has been established and characterized. This cell line, designated as EK (eel kidney), has been maintained in Leibovitz's L-15 supplemented with 10% fetal bovine serum for over 24 months, and subcultured more than 60 ti...
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| Format: | Article |
| Language: | English |
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The Company of Biologists
2019-01-01
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| Series: | Biology Open |
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| Online Access: | http://bio.biologists.org/content/8/1/bio037507 |
| _version_ | 1818900964457840640 |
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| author | Bin Chen Zaiyu Zheng Jinxian Yang Hongshu Chi He Huang Hui Gong |
| author_facet | Bin Chen Zaiyu Zheng Jinxian Yang Hongshu Chi He Huang Hui Gong |
| author_sort | Bin Chen |
| collection | DOAJ |
| description | A new cell line derived from the kidney of European eel, Anguilla anguilla, has been established and characterized. This cell line, designated as EK (eel kidney), has been maintained in Leibovitz's L-15 supplemented with 10% fetal bovine serum for over 24 months, and subcultured more than 60 times. This cell line consists predominantly of fibroblast-like cells, and can grow at 15–37°C under an optimum temperature of 26°C. The origin of this cell line was confirmed by polymerase chain reaction (PCR) amplification and 18s recombinant (r)RNA sequencing. The chromosome analysis of EK cells at passage 58 revealed an ananeuploid karyotype. The EK cells were successfully transfected with the Pegfp-N1 plasmid, suggesting its potential in genetic studies. The susceptibility test showed a significant cytopathic effect (CPE) in EK cells for Rana grylio virus, and the viral replication was evidenced with quantitative real-time PCR (qRT-PCR) assay. After poly (I:C) stimulation, the expression of the immune-related molecules including interferon regulatory factor-3 (irf3), interferon regulatory factor-7 (irf7) and cytochrome P450 (CYP450) were significantly upregulated in EK cells, while the expression of transforming growth factor (TGF-β) was downregulated. These results suggested the potential of EK cell line as a model in gene engineering, virus identification and environmental toxicology. |
| first_indexed | 2024-12-19T20:12:13Z |
| format | Article |
| id | doaj.art-bfdcfc655711441cb1f78b795592088e |
| institution | Directory Open Access Journal |
| issn | 2046-6390 |
| language | English |
| last_indexed | 2024-12-19T20:12:13Z |
| publishDate | 2019-01-01 |
| publisher | The Company of Biologists |
| record_format | Article |
| series | Biology Open |
| spelling | doaj.art-bfdcfc655711441cb1f78b795592088e2022-12-21T20:07:17ZengThe Company of BiologistsBiology Open2046-63902019-01-018110.1242/bio.037507037507Development and characterization of a new cell line derived from European eel Anguilla anguilla kidneyBin Chen0Zaiyu Zheng1Jinxian Yang2Hongshu Chi3He Huang4Hui Gong5 Biotechnology Institute, Fujian Academy of Agricultural Sciences, High-tech building 1506, Wusi Road 247, Fuzhou 350003, Fujian, China Biotechnology Institute, Fujian Academy of Agricultural Sciences, High-tech building 1506, Wusi Road 247, Fuzhou 350003, Fujian, China Biotechnology Institute, Fujian Academy of Agricultural Sciences, High-tech building 1506, Wusi Road 247, Fuzhou 350003, Fujian, China Biotechnology Institute, Fujian Academy of Agricultural Sciences, High-tech building 1506, Wusi Road 247, Fuzhou 350003, Fujian, China Biotechnology Institute, Fujian Academy of Agricultural Sciences, High-tech building 1506, Wusi Road 247, Fuzhou 350003, Fujian, China Biotechnology Institute, Fujian Academy of Agricultural Sciences, High-tech building 1506, Wusi Road 247, Fuzhou 350003, Fujian, China A new cell line derived from the kidney of European eel, Anguilla anguilla, has been established and characterized. This cell line, designated as EK (eel kidney), has been maintained in Leibovitz's L-15 supplemented with 10% fetal bovine serum for over 24 months, and subcultured more than 60 times. This cell line consists predominantly of fibroblast-like cells, and can grow at 15–37°C under an optimum temperature of 26°C. The origin of this cell line was confirmed by polymerase chain reaction (PCR) amplification and 18s recombinant (r)RNA sequencing. The chromosome analysis of EK cells at passage 58 revealed an ananeuploid karyotype. The EK cells were successfully transfected with the Pegfp-N1 plasmid, suggesting its potential in genetic studies. The susceptibility test showed a significant cytopathic effect (CPE) in EK cells for Rana grylio virus, and the viral replication was evidenced with quantitative real-time PCR (qRT-PCR) assay. After poly (I:C) stimulation, the expression of the immune-related molecules including interferon regulatory factor-3 (irf3), interferon regulatory factor-7 (irf7) and cytochrome P450 (CYP450) were significantly upregulated in EK cells, while the expression of transforming growth factor (TGF-β) was downregulated. These results suggested the potential of EK cell line as a model in gene engineering, virus identification and environmental toxicology.http://bio.biologists.org/content/8/1/bio037507Anguilla anguillaKidney cell lineRana grylio virusImmune-related gene |
| spellingShingle | Bin Chen Zaiyu Zheng Jinxian Yang Hongshu Chi He Huang Hui Gong Development and characterization of a new cell line derived from European eel Anguilla anguilla kidney Biology Open Anguilla anguilla Kidney cell line Rana grylio virus Immune-related gene |
| title | Development and characterization of a new cell line derived from European eel Anguilla anguilla kidney |
| title_full | Development and characterization of a new cell line derived from European eel Anguilla anguilla kidney |
| title_fullStr | Development and characterization of a new cell line derived from European eel Anguilla anguilla kidney |
| title_full_unstemmed | Development and characterization of a new cell line derived from European eel Anguilla anguilla kidney |
| title_short | Development and characterization of a new cell line derived from European eel Anguilla anguilla kidney |
| title_sort | development and characterization of a new cell line derived from european eel anguilla anguilla kidney |
| topic | Anguilla anguilla Kidney cell line Rana grylio virus Immune-related gene |
| url | http://bio.biologists.org/content/8/1/bio037507 |
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