A human-blood-derived microRNA facilitates flavivirus infection in fed mosquitoes
Summary: Hematophagous arthropods, such as mosquitoes, naturally carry and transmit hundreds of arboviruses to humans. Blood meal is a predominant physical interface that shapes cross-species communications among humans, bloodsuckers, and arboviruses. Here, we identify a human-blood-derived microRNA...
| Main Authors: | , , , , , , , , |
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| Format: | Article |
| Language: | English |
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Elsevier
2021-12-01
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| Series: | Cell Reports |
| Subjects: | |
| Online Access: | http://www.sciencedirect.com/science/article/pii/S2211124721015825 |
| _version_ | 1819319560350728192 |
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| author | Yibin Zhu Chi Zhang Liming Zhang Yun Yang Xi Yu Jinglin Wang Qiyong Liu Penghua Wang Gong Cheng |
| author_facet | Yibin Zhu Chi Zhang Liming Zhang Yun Yang Xi Yu Jinglin Wang Qiyong Liu Penghua Wang Gong Cheng |
| author_sort | Yibin Zhu |
| collection | DOAJ |
| description | Summary: Hematophagous arthropods, such as mosquitoes, naturally carry and transmit hundreds of arboviruses to humans. Blood meal is a predominant physical interface that shapes cross-species communications among humans, bloodsuckers, and arboviruses. Here, we identify a human-blood-derived microRNA, hsa-miR-150-5p, that interferes with a mosquito antiviral system to facilitate flavivirus infection and transmission. hsa-miR-150-5p is acquired with a blood meal into the mosquito hemocoel and persists for a prolonged time there. The agomir of hsa-miR-150-5p enhances, whereas the antagomir represses flaviviral infection in mosquitoes and transmission from mice to mosquitoes. Mechanistic studies indicate that hsa-miR-150-5p hijacks the mosquito Argonaute-1-mediated RNA interference system to suppress the expression of some chymotrypsins with potent virucidal activity. Mosquito chymotrypsins are essential for resisting systemic flavivirus infection in hemocoel tissues. Chymotrypsin homologs potentially targeted by miR-150-5p are also found in other hematophagous arthropods, demonstrating a conserved miR-150-5p-mediated cross-species RNAi mechanism that might determine flaviviral transmissibility in nature. |
| first_indexed | 2024-12-24T11:05:38Z |
| format | Article |
| id | doaj.art-bfdd67d1a2424077918f8c5ad38a2fb9 |
| institution | Directory Open Access Journal |
| issn | 2211-1247 |
| language | English |
| last_indexed | 2024-12-24T11:05:38Z |
| publishDate | 2021-12-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Cell Reports |
| spelling | doaj.art-bfdd67d1a2424077918f8c5ad38a2fb92022-12-21T16:58:37ZengElsevierCell Reports2211-12472021-12-013711110091A human-blood-derived microRNA facilitates flavivirus infection in fed mosquitoesYibin Zhu0Chi Zhang1Liming Zhang2Yun Yang3Xi Yu4Jinglin Wang5Qiyong Liu6Penghua Wang7Gong Cheng8Tsinghua-Peking Center for Life Sciences, School of Medicine, Tsinghua University, Beijing 100084, China; Institute of Infectious Diseases, Shenzhen Bay Laboratory, Shenzhen, Guangdong 518000, China; Institute of Pathogenic Organisms, Shenzhen Center for Disease Control and Prevention, Shenzhen, Guangdong 518055, ChinaTsinghua-Peking Center for Life Sciences, School of Medicine, Tsinghua University, Beijing 100084, ChinaTsinghua-Peking Center for Life Sciences, School of Medicine, Tsinghua University, Beijing 100084, ChinaTsinghua-Peking Center for Life Sciences, School of Medicine, Tsinghua University, Beijing 100084, ChinaTsinghua-Peking Center for Life Sciences, School of Medicine, Tsinghua University, Beijing 100084, ChinaYunnan Tropical and Subtropical Animal Viral Disease Laboratory, Yunnan Animal Science and Veterinary Institute, Kunming 650224, ChinaState Key Laboratory of Infectious Disease Prevention and Control, Beijing 102206, ChinaDepartment of Immunology, School of Medicine, the University of Connecticut Health Center, Farmington, CT 06030, USATsinghua-Peking Center for Life Sciences, School of Medicine, Tsinghua University, Beijing 100084, China; Institute of Infectious Diseases, Shenzhen Bay Laboratory, Shenzhen, Guangdong 518000, China; Institute of Pathogenic Organisms, Shenzhen Center for Disease Control and Prevention, Shenzhen, Guangdong 518055, China; Corresponding authorSummary: Hematophagous arthropods, such as mosquitoes, naturally carry and transmit hundreds of arboviruses to humans. Blood meal is a predominant physical interface that shapes cross-species communications among humans, bloodsuckers, and arboviruses. Here, we identify a human-blood-derived microRNA, hsa-miR-150-5p, that interferes with a mosquito antiviral system to facilitate flavivirus infection and transmission. hsa-miR-150-5p is acquired with a blood meal into the mosquito hemocoel and persists for a prolonged time there. The agomir of hsa-miR-150-5p enhances, whereas the antagomir represses flaviviral infection in mosquitoes and transmission from mice to mosquitoes. Mechanistic studies indicate that hsa-miR-150-5p hijacks the mosquito Argonaute-1-mediated RNA interference system to suppress the expression of some chymotrypsins with potent virucidal activity. Mosquito chymotrypsins are essential for resisting systemic flavivirus infection in hemocoel tissues. Chymotrypsin homologs potentially targeted by miR-150-5p are also found in other hematophagous arthropods, demonstrating a conserved miR-150-5p-mediated cross-species RNAi mechanism that might determine flaviviral transmissibility in nature.http://www.sciencedirect.com/science/article/pii/S2211124721015825flavivirusmosquitomiRNAcross-species |
| spellingShingle | Yibin Zhu Chi Zhang Liming Zhang Yun Yang Xi Yu Jinglin Wang Qiyong Liu Penghua Wang Gong Cheng A human-blood-derived microRNA facilitates flavivirus infection in fed mosquitoes Cell Reports flavivirus mosquito miRNA cross-species |
| title | A human-blood-derived microRNA facilitates flavivirus infection in fed mosquitoes |
| title_full | A human-blood-derived microRNA facilitates flavivirus infection in fed mosquitoes |
| title_fullStr | A human-blood-derived microRNA facilitates flavivirus infection in fed mosquitoes |
| title_full_unstemmed | A human-blood-derived microRNA facilitates flavivirus infection in fed mosquitoes |
| title_short | A human-blood-derived microRNA facilitates flavivirus infection in fed mosquitoes |
| title_sort | human blood derived microrna facilitates flavivirus infection in fed mosquitoes |
| topic | flavivirus mosquito miRNA cross-species |
| url | http://www.sciencedirect.com/science/article/pii/S2211124721015825 |
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