Mechanism of Vasorelaxation Induced by Coptosapelta flavescens Stems Extract in Rat Thoraric Aorta
Coptosapelta flavescens Korth. is a liana plant from the Rubiaceae family. In East Kalimantan, it is also known as “Akar Tambolekar†or “Merungâ€, it is called “akar†or root as its trunk spreads like a root. The plant’s stems are used by ethnic Dayak in East Kalimantan to overcome high bl...
| Main Authors: | , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Mulawarman University
2017-12-01
|
| Series: | Journal of Tropical Pharmacy and Chemistry |
| Subjects: | |
| Online Access: | https://jtpc.farmasi.unmul.ac.id/index.php/jtpc/article/view/146 |
| _version_ | 1827771108578820096 |
|---|---|
| author | Sjarif Ismail Khemasili Kosala Ika Fikriah Agustina Rahayu Magdaleni |
| author_facet | Sjarif Ismail Khemasili Kosala Ika Fikriah Agustina Rahayu Magdaleni |
| author_sort | Sjarif Ismail |
| collection | DOAJ |
| description | Coptosapelta flavescens Korth. is a liana plant from the Rubiaceae family. In East Kalimantan, it is also known as “Akar Tambolekar†or “Merungâ€, it is called “akar†or root as its trunk spreads like a root. The plant’s stems are used by ethnic Dayak in East Kalimantan to overcome high blood pressure and it has been proven to induce vasorelaxation on blood vessels, but the its action mechanism in the endothelial or vascular smooth muscle cells (VSMC) are unknown. Vasorelaxation on the blood vessels could be mediated by endothelial or by the VSMC. This research aims to study in vitro which of them is the mechanism of action of Coptosapelta flavescens Stems (CFS) extract. CFS were taken from the secondary forest in Paser Regency, East Kalimantan Province. Its simplicia were macerated with methanol solvent for three days and twice repeated. Vasorelaxation activity of the blood vessels was tested with rats’ isolated thoracic aorta with endothelial and with the endothelial removed (endothelium-denuded). Both aorta of 3 mm length were soaked into Krebs-Henselheit solution at 37°C, pH 7.4 and aerated with carbogen gas. After they have acclimatized, both aortas were contracted with phenylephrine solution, after reaching peak contraction and plateau, the solution of extract or its solvent (Control) was administered in cumulatively increasing doses. The results show that CFS extracts induce vasorelaxation both in the endothelial-intact aorta and in the endothelial-denuded aorta. At high concentrations, the vasorelaxation activity in the endothelial-intact aorta was weaker than that on endothelial-denuded aorta. This study proves that action mechanism of blood vessels’ vasorelaxation induced by the methanol extract of CFS were more dominantly mediated by the blood vessels smooth muscle; and at high concentrations the endothelial actually weakened the VSMC vasorelaxation activity. Further study is necessary on the mechanism of action vasorelaxation through the modulation of intracellular or extracellular calcium ion channels of VSMC
Keywords: Coptosapelta flavescens, vasorelaxation, extract, aorta, in vitro
|
| first_indexed | 2024-03-11T12:49:00Z |
| format | Article |
| id | doaj.art-bfe0c65ee6c24c6882f5c00002da0fd7 |
| institution | Directory Open Access Journal |
| issn | 2087-7099 2407-6090 |
| language | English |
| last_indexed | 2024-03-11T12:49:00Z |
| publishDate | 2017-12-01 |
| publisher | Mulawarman University |
| record_format | Article |
| series | Journal of Tropical Pharmacy and Chemistry |
| spelling | doaj.art-bfe0c65ee6c24c6882f5c00002da0fd72023-11-04T06:12:20ZengMulawarman UniversityJournal of Tropical Pharmacy and Chemistry2087-70992407-60902017-12-014210.25026/jtpc.v4i2.146146Mechanism of Vasorelaxation Induced by Coptosapelta flavescens Stems Extract in Rat Thoraric AortaSjarif Ismail0Khemasili Kosala1Ika Fikriah2Agustina Rahayu Magdaleni3Research Center on Drugs and Public Health, Mulawarman University, Samarinda, IndonesiaPharmacology Laboratory, Faculty of Medicine Mulawarman University, Samarinda, IndonesiaPharmacology Laboratory, Faculty of Medicine, Mulawarman University, Samarinda, IndonesiaPhysiology Laboratory, Faculty of Medicine, Mulawarman University, Samarinda, IndonesiaCoptosapelta flavescens Korth. is a liana plant from the Rubiaceae family. In East Kalimantan, it is also known as “Akar Tambolekar†or “Merungâ€, it is called “akar†or root as its trunk spreads like a root. The plant’s stems are used by ethnic Dayak in East Kalimantan to overcome high blood pressure and it has been proven to induce vasorelaxation on blood vessels, but the its action mechanism in the endothelial or vascular smooth muscle cells (VSMC) are unknown. Vasorelaxation on the blood vessels could be mediated by endothelial or by the VSMC. This research aims to study in vitro which of them is the mechanism of action of Coptosapelta flavescens Stems (CFS) extract. CFS were taken from the secondary forest in Paser Regency, East Kalimantan Province. Its simplicia were macerated with methanol solvent for three days and twice repeated. Vasorelaxation activity of the blood vessels was tested with rats’ isolated thoracic aorta with endothelial and with the endothelial removed (endothelium-denuded). Both aorta of 3 mm length were soaked into Krebs-Henselheit solution at 37°C, pH 7.4 and aerated with carbogen gas. After they have acclimatized, both aortas were contracted with phenylephrine solution, after reaching peak contraction and plateau, the solution of extract or its solvent (Control) was administered in cumulatively increasing doses. The results show that CFS extracts induce vasorelaxation both in the endothelial-intact aorta and in the endothelial-denuded aorta. At high concentrations, the vasorelaxation activity in the endothelial-intact aorta was weaker than that on endothelial-denuded aorta. This study proves that action mechanism of blood vessels’ vasorelaxation induced by the methanol extract of CFS were more dominantly mediated by the blood vessels smooth muscle; and at high concentrations the endothelial actually weakened the VSMC vasorelaxation activity. Further study is necessary on the mechanism of action vasorelaxation through the modulation of intracellular or extracellular calcium ion channels of VSMC Keywords: Coptosapelta flavescens, vasorelaxation, extract, aorta, in vitro https://jtpc.farmasi.unmul.ac.id/index.php/jtpc/article/view/146Keywords: Coptosapelta flavescens, vasorelaxation, extract, aorta, in vitro |
| spellingShingle | Sjarif Ismail Khemasili Kosala Ika Fikriah Agustina Rahayu Magdaleni Mechanism of Vasorelaxation Induced by Coptosapelta flavescens Stems Extract in Rat Thoraric Aorta Journal of Tropical Pharmacy and Chemistry Keywords: Coptosapelta flavescens, vasorelaxation, extract, aorta, in vitro |
| title | Mechanism of Vasorelaxation Induced by Coptosapelta flavescens Stems Extract in Rat Thoraric Aorta |
| title_full | Mechanism of Vasorelaxation Induced by Coptosapelta flavescens Stems Extract in Rat Thoraric Aorta |
| title_fullStr | Mechanism of Vasorelaxation Induced by Coptosapelta flavescens Stems Extract in Rat Thoraric Aorta |
| title_full_unstemmed | Mechanism of Vasorelaxation Induced by Coptosapelta flavescens Stems Extract in Rat Thoraric Aorta |
| title_short | Mechanism of Vasorelaxation Induced by Coptosapelta flavescens Stems Extract in Rat Thoraric Aorta |
| title_sort | mechanism of vasorelaxation induced by coptosapelta flavescens stems extract in rat thoraric aorta |
| topic | Keywords: Coptosapelta flavescens, vasorelaxation, extract, aorta, in vitro |
| url | https://jtpc.farmasi.unmul.ac.id/index.php/jtpc/article/view/146 |
| work_keys_str_mv | AT sjarifismail mechanismofvasorelaxationinducedbycoptosapeltaflavescensstemsextractinratthoraricaorta AT khemasilikosala mechanismofvasorelaxationinducedbycoptosapeltaflavescensstemsextractinratthoraricaorta AT ikafikriah mechanismofvasorelaxationinducedbycoptosapeltaflavescensstemsextractinratthoraricaorta AT agustinarahayumagdaleni mechanismofvasorelaxationinducedbycoptosapeltaflavescensstemsextractinratthoraricaorta |