Evaluation of the Effect of MTAD on Expression of Enterococcus faecalis Virulence Factors Considering the Role of Different Obturating Materials
Objectives: The aim of this study was to determine the effect of MTAD on the expression of virulence factors of Enterococcus faecalis (E.faecalis) considering the role of Gutta-percha/AH26 or Resilon/RealSeal SE as root canal obturating materials. Materials and Methods: One-hundred and forty-four si...
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| Format: | Article |
| Language: | English |
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Tehran University of Medical Sciences
2018-11-01
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| Series: | Frontiers in Dentistry |
| Subjects: | |
| Online Access: | https://jdt.tums.ac.ir/index.php/jdt/article/view/2592 |
| _version_ | 1818145017425821696 |
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| author | Behnam Bolhari Abbas Bahador Mehrfam Khoshkhounejad Mahsa Sobhi Afshar Mohammad Moghaddaszadeh |
| author_facet | Behnam Bolhari Abbas Bahador Mehrfam Khoshkhounejad Mahsa Sobhi Afshar Mohammad Moghaddaszadeh |
| author_sort | Behnam Bolhari |
| collection | DOAJ |
| description | Objectives: The aim of this study was to determine the effect of MTAD on the expression of virulence factors of Enterococcus faecalis (E.faecalis) considering the role of Gutta-percha/AH26 or Resilon/RealSeal SE as root canal obturating materials.
Materials and Methods: One-hundred and forty-four single-rooted human teeth were instrumented to a standardized apical size. Root canals were infected by E.faecalis (ATCC 29212). Ninety teeth were irrigated with MTAD and randomly divided into three groups. In two groups, root canals were obturated by either Gutta-percha/AH26 or Resilon/RealSeal SE. Root canals were kept unobturated in the third group. The remaining 54 teeth received no final irrigation. All groups were then subdivided into three timepoint subgroups in which dentin powder was obtained from each sample to determine the expression of specific virulence factors of E.faecalis (efa, esp, gel, fsr) using real-time reverse transcription polymerase chain reaction (RT-PCR). Statistical analysis was performed by one-way analysis of variance (ANOVA) and Tukey’s post-hoc test. The statistical power was set at P-value ≤0.05.
Results: MTAD was effective against the expression of most of the tested virulence factors, and Gutta-percha/AH26 increased the antibacterial efficacy of MTAD.
Conclusions: MTAD could inhibit the expression of some known virulence factors of E.faecalis at the majority of tested timepoints. This may partly explain some of the mechanisms of antimicrobial efficacy of MTAD against this resistant microorganism which is known as one of the main causes of failure of root canal treatment. |
| first_indexed | 2024-12-11T11:56:46Z |
| format | Article |
| id | doaj.art-bfe18b95009041f48486792c5728900e |
| institution | Directory Open Access Journal |
| issn | 2676-296X |
| language | English |
| last_indexed | 2024-12-11T11:56:46Z |
| publishDate | 2018-11-01 |
| publisher | Tehran University of Medical Sciences |
| record_format | Article |
| series | Frontiers in Dentistry |
| spelling | doaj.art-bfe18b95009041f48486792c5728900e2022-12-22T01:08:11ZengTehran University of Medical SciencesFrontiers in Dentistry2676-296X2018-11-011562592Evaluation of the Effect of MTAD on Expression of Enterococcus faecalis Virulence Factors Considering the Role of Different Obturating MaterialsBehnam Bolhari0Abbas Bahador1Mehrfam Khoshkhounejad2Mahsa Sobhi Afshar3Mohammad Moghaddaszadeh4Associate Professor, Department of Endodontics, School of Dentistry, Tehran University of Medical Sciences, Tehran, IranAssociate professor, Department of Microbiology, School of Medicine, Tehran University of Medical Sciences, Tehran, IranAssistant Professor, Department of Endodontics, School of Dentistry, Tehran University of Medical Sciences, Tehran, IranAssistant Professor, Department of Endodontics, School of Dentistry, Tehran University of Medical Sciences, Tehran, IranPrivate Practice, Tehran, IranObjectives: The aim of this study was to determine the effect of MTAD on the expression of virulence factors of Enterococcus faecalis (E.faecalis) considering the role of Gutta-percha/AH26 or Resilon/RealSeal SE as root canal obturating materials. Materials and Methods: One-hundred and forty-four single-rooted human teeth were instrumented to a standardized apical size. Root canals were infected by E.faecalis (ATCC 29212). Ninety teeth were irrigated with MTAD and randomly divided into three groups. In two groups, root canals were obturated by either Gutta-percha/AH26 or Resilon/RealSeal SE. Root canals were kept unobturated in the third group. The remaining 54 teeth received no final irrigation. All groups were then subdivided into three timepoint subgroups in which dentin powder was obtained from each sample to determine the expression of specific virulence factors of E.faecalis (efa, esp, gel, fsr) using real-time reverse transcription polymerase chain reaction (RT-PCR). Statistical analysis was performed by one-way analysis of variance (ANOVA) and Tukey’s post-hoc test. The statistical power was set at P-value ≤0.05. Results: MTAD was effective against the expression of most of the tested virulence factors, and Gutta-percha/AH26 increased the antibacterial efficacy of MTAD. Conclusions: MTAD could inhibit the expression of some known virulence factors of E.faecalis at the majority of tested timepoints. This may partly explain some of the mechanisms of antimicrobial efficacy of MTAD against this resistant microorganism which is known as one of the main causes of failure of root canal treatment.https://jdt.tums.ac.ir/index.php/jdt/article/view/2592Enterococcus faecalisGutta-PerchaMTADResilon SealerVirulence Factors |
| spellingShingle | Behnam Bolhari Abbas Bahador Mehrfam Khoshkhounejad Mahsa Sobhi Afshar Mohammad Moghaddaszadeh Evaluation of the Effect of MTAD on Expression of Enterococcus faecalis Virulence Factors Considering the Role of Different Obturating Materials Frontiers in Dentistry Enterococcus faecalis Gutta-Percha MTAD Resilon Sealer Virulence Factors |
| title | Evaluation of the Effect of MTAD on Expression of Enterococcus faecalis Virulence Factors Considering the Role of Different Obturating Materials |
| title_full | Evaluation of the Effect of MTAD on Expression of Enterococcus faecalis Virulence Factors Considering the Role of Different Obturating Materials |
| title_fullStr | Evaluation of the Effect of MTAD on Expression of Enterococcus faecalis Virulence Factors Considering the Role of Different Obturating Materials |
| title_full_unstemmed | Evaluation of the Effect of MTAD on Expression of Enterococcus faecalis Virulence Factors Considering the Role of Different Obturating Materials |
| title_short | Evaluation of the Effect of MTAD on Expression of Enterococcus faecalis Virulence Factors Considering the Role of Different Obturating Materials |
| title_sort | evaluation of the effect of mtad on expression of enterococcus faecalis virulence factors considering the role of different obturating materials |
| topic | Enterococcus faecalis Gutta-Percha MTAD Resilon Sealer Virulence Factors |
| url | https://jdt.tums.ac.ir/index.php/jdt/article/view/2592 |
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