Biosensors for the Detection of Interaction between Legionella pneumophila Collagen-Like Protein and Glycosaminoglycans
The adhesin Legionella collagen-like (Lcl) protein can bind to extracellular matrix components and mediate the binding of Legionella pneumophila to host cells. In this study, electrochemical impedance spectroscopy (EIS) and surface plasmon resonance (SPR)-based biosensors were employed to characteri...
| Main Authors: | , , , , |
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| Format: | Article |
| Language: | English |
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MDPI AG
2018-08-01
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| Series: | Sensors |
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| Online Access: | http://www.mdpi.com/1424-8220/18/8/2668 |
| _version_ | 1797999565364789248 |
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| author | Han Su Shaopei Li Mauricio Terebiznik Cyril Guyard Kagan Kerman |
| author_facet | Han Su Shaopei Li Mauricio Terebiznik Cyril Guyard Kagan Kerman |
| author_sort | Han Su |
| collection | DOAJ |
| description | The adhesin Legionella collagen-like (Lcl) protein can bind to extracellular matrix components and mediate the binding of Legionella pneumophila to host cells. In this study, electrochemical impedance spectroscopy (EIS) and surface plasmon resonance (SPR)-based biosensors were employed to characterize these interactions between glycosaminoglycans (GAGs) and the adhesin Lcl protein. Fucoidan displayed a high affinity (KD 18 nM) for Lcl protein. Chondroitin sulfate A and dermatan sulfate differ in the position of a carboxyl group replacing D-glucuronate with D-iduronate. Our results indicated that the presence of D-iduronate in dermatan sulfate strongly hindered its interaction with Lcl. These biophysical studies provided valuable information in our understanding of adhesin-ligand interactions related to Legionella pneumophila infections. |
| first_indexed | 2024-04-11T11:06:42Z |
| format | Article |
| id | doaj.art-bfe25ae4aea74510ad7df5e04655b901 |
| institution | Directory Open Access Journal |
| issn | 1424-8220 |
| language | English |
| last_indexed | 2024-04-11T11:06:42Z |
| publishDate | 2018-08-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Sensors |
| spelling | doaj.art-bfe25ae4aea74510ad7df5e04655b9012022-12-22T04:28:20ZengMDPI AGSensors1424-82202018-08-01188266810.3390/s18082668s18082668Biosensors for the Detection of Interaction between Legionella pneumophila Collagen-Like Protein and GlycosaminoglycansHan Su0Shaopei Li1Mauricio Terebiznik2Cyril Guyard3Kagan Kerman4Department of Physical and Environmental Sciences, University of Toronto Scarborough, 1265 Military Trail, Toronto, ON M1C 1A4, CanadaDepartment of Physical and Environmental Sciences, University of Toronto Scarborough, 1265 Military Trail, Toronto, ON M1C 1A4, CanadaDepartment of Biological Sciences and Cell and Systems Biology, University of Toronto Scarborough, 1265 Military Trail, Toronto, ON M1C 1A4, CanadaBIOASTER Microbiology Technology Institute, 40 Avenue Tony Garnier, 69007 Lyon, FranceDepartment of Physical and Environmental Sciences, University of Toronto Scarborough, 1265 Military Trail, Toronto, ON M1C 1A4, CanadaThe adhesin Legionella collagen-like (Lcl) protein can bind to extracellular matrix components and mediate the binding of Legionella pneumophila to host cells. In this study, electrochemical impedance spectroscopy (EIS) and surface plasmon resonance (SPR)-based biosensors were employed to characterize these interactions between glycosaminoglycans (GAGs) and the adhesin Lcl protein. Fucoidan displayed a high affinity (KD 18 nM) for Lcl protein. Chondroitin sulfate A and dermatan sulfate differ in the position of a carboxyl group replacing D-glucuronate with D-iduronate. Our results indicated that the presence of D-iduronate in dermatan sulfate strongly hindered its interaction with Lcl. These biophysical studies provided valuable information in our understanding of adhesin-ligand interactions related to Legionella pneumophila infections.http://www.mdpi.com/1424-8220/18/8/2668Legionella collagen-like proteinglycosaminoglycansfucoidansurface plasmon resonanceelectrochemical impedance spectroscopy |
| spellingShingle | Han Su Shaopei Li Mauricio Terebiznik Cyril Guyard Kagan Kerman Biosensors for the Detection of Interaction between Legionella pneumophila Collagen-Like Protein and Glycosaminoglycans Sensors Legionella collagen-like protein glycosaminoglycans fucoidan surface plasmon resonance electrochemical impedance spectroscopy |
| title | Biosensors for the Detection of Interaction between Legionella pneumophila Collagen-Like Protein and Glycosaminoglycans |
| title_full | Biosensors for the Detection of Interaction between Legionella pneumophila Collagen-Like Protein and Glycosaminoglycans |
| title_fullStr | Biosensors for the Detection of Interaction between Legionella pneumophila Collagen-Like Protein and Glycosaminoglycans |
| title_full_unstemmed | Biosensors for the Detection of Interaction between Legionella pneumophila Collagen-Like Protein and Glycosaminoglycans |
| title_short | Biosensors for the Detection of Interaction between Legionella pneumophila Collagen-Like Protein and Glycosaminoglycans |
| title_sort | biosensors for the detection of interaction between legionella pneumophila collagen like protein and glycosaminoglycans |
| topic | Legionella collagen-like protein glycosaminoglycans fucoidan surface plasmon resonance electrochemical impedance spectroscopy |
| url | http://www.mdpi.com/1424-8220/18/8/2668 |
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