Screening of faba bean (Vicia faba L.) accessions to acidity and aluminium stresses
Background Faba bean is an important starch-based protein crop produced worldwide. Soil acidity and aluminium toxicity are major abiotic stresses affecting its production, so in regions where soil acidity is a problem, there is a gap between the potential and actual productivity of the crop. Hence,...
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PeerJ Inc.
2017-02-01
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| Online Access: | https://peerj.com/articles/2963.pdf |
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| author | Kiflemariam Y. Belachew Frederick L. Stoddard |
| author_facet | Kiflemariam Y. Belachew Frederick L. Stoddard |
| author_sort | Kiflemariam Y. Belachew |
| collection | DOAJ |
| description | Background Faba bean is an important starch-based protein crop produced worldwide. Soil acidity and aluminium toxicity are major abiotic stresses affecting its production, so in regions where soil acidity is a problem, there is a gap between the potential and actual productivity of the crop. Hence, we set out to evaluate acidity and aluminium tolerance in a range of faba bean germplasm using solution culture and pot experiments. Methods A set of 30 accessions was collected from regions where acidity and aluminium are or are not problems. The accessions were grown in solution culture and a subset of 10 was grown first in peat and later in perlite potting media. In solution culture, morphological parameters including taproot length, root regrowth and root tolerance index were measured, and in the pot experiments the key measurements were taproot length, plant biomass, chlorophyll concentration and stomatal conductance. Result Responses to acidity and aluminium were apparently independent. Accessions Dosha and NC 58 were tolerant to both stress. Kassa and GLA 1103 were tolerant to acidity showing less than 3% reduction in taproot length. Aurora and Messay were tolerant to aluminium. Babylon was sensitive to both, with up to 40% reduction in taproot length from acidity and no detectable recovery from Al3+ challenge. Discussion The apparent independence of the responses to acidity and aluminium is in agreement with the previous research findings, suggesting that crop accessions separately adapt to H+ and Al3+ toxicity as a result of the difference in the nature of soil parent materials where the accession originated. Differences in rankings between experiments were minor and attributable to heterogeneity of seed materials and the specific responses of accessions to the rooting media. Use of perlite as a potting medium offers an ideal combination of throughput, inertness of support medium, access to leaves for detection of their stress responses, and harvest of clean roots for evaluation of their growth. |
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| format | Article |
| id | doaj.art-bfe2ffcd2de84dcab6af20c9fec5349e |
| institution | Directory Open Access Journal |
| issn | 2167-8359 |
| language | English |
| last_indexed | 2024-03-09T07:24:21Z |
| publishDate | 2017-02-01 |
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| spelling | doaj.art-bfe2ffcd2de84dcab6af20c9fec5349e2023-12-03T07:08:54ZengPeerJ Inc.PeerJ2167-83592017-02-015e296310.7717/peerj.2963Screening of faba bean (Vicia faba L.) accessions to acidity and aluminium stressesKiflemariam Y. Belachew0Frederick L. Stoddard1Department of Agricultural Sciences, Viikki Plant Science Centre, University of Helsinki, Helsinki, South Finland, FinlandDepartment of Food and Environmental Sciences, Viikki Plant Science Centre, University of Helsinki, Helsinki, FinlandBackground Faba bean is an important starch-based protein crop produced worldwide. Soil acidity and aluminium toxicity are major abiotic stresses affecting its production, so in regions where soil acidity is a problem, there is a gap between the potential and actual productivity of the crop. Hence, we set out to evaluate acidity and aluminium tolerance in a range of faba bean germplasm using solution culture and pot experiments. Methods A set of 30 accessions was collected from regions where acidity and aluminium are or are not problems. The accessions were grown in solution culture and a subset of 10 was grown first in peat and later in perlite potting media. In solution culture, morphological parameters including taproot length, root regrowth and root tolerance index were measured, and in the pot experiments the key measurements were taproot length, plant biomass, chlorophyll concentration and stomatal conductance. Result Responses to acidity and aluminium were apparently independent. Accessions Dosha and NC 58 were tolerant to both stress. Kassa and GLA 1103 were tolerant to acidity showing less than 3% reduction in taproot length. Aurora and Messay were tolerant to aluminium. Babylon was sensitive to both, with up to 40% reduction in taproot length from acidity and no detectable recovery from Al3+ challenge. Discussion The apparent independence of the responses to acidity and aluminium is in agreement with the previous research findings, suggesting that crop accessions separately adapt to H+ and Al3+ toxicity as a result of the difference in the nature of soil parent materials where the accession originated. Differences in rankings between experiments were minor and attributable to heterogeneity of seed materials and the specific responses of accessions to the rooting media. Use of perlite as a potting medium offers an ideal combination of throughput, inertness of support medium, access to leaves for detection of their stress responses, and harvest of clean roots for evaluation of their growth.https://peerj.com/articles/2963.pdfFaba beanViciaToxicityAcidScreeningAluminium |
| spellingShingle | Kiflemariam Y. Belachew Frederick L. Stoddard Screening of faba bean (Vicia faba L.) accessions to acidity and aluminium stresses PeerJ Faba bean Vicia Toxicity Acid Screening Aluminium |
| title | Screening of faba bean (Vicia faba L.) accessions to acidity and aluminium stresses |
| title_full | Screening of faba bean (Vicia faba L.) accessions to acidity and aluminium stresses |
| title_fullStr | Screening of faba bean (Vicia faba L.) accessions to acidity and aluminium stresses |
| title_full_unstemmed | Screening of faba bean (Vicia faba L.) accessions to acidity and aluminium stresses |
| title_short | Screening of faba bean (Vicia faba L.) accessions to acidity and aluminium stresses |
| title_sort | screening of faba bean vicia faba l accessions to acidity and aluminium stresses |
| topic | Faba bean Vicia Toxicity Acid Screening Aluminium |
| url | https://peerj.com/articles/2963.pdf |
| work_keys_str_mv | AT kiflemariamybelachew screeningoffababeanviciafabalaccessionstoacidityandaluminiumstresses AT fredericklstoddard screeningoffababeanviciafabalaccessionstoacidityandaluminiumstresses |