Production of wheat-doubled haploids resistant to eyespot supported by marker-assisted selection
Background: Wheat is one of the most important crops cultivated all over the world. New high-yielding cultivars that are more resistant to fungal diseases have been permanently developed. The present study aimed at the possibility of accelerating the process of breeding new cultivars, resistant to e...
| Main Authors: | , , , , , , , , , , |
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| Format: | Article |
| Language: | English |
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Elsevier
2019-01-01
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| Series: | Electronic Journal of Biotechnology |
| Online Access: | http://www.sciencedirect.com/science/article/pii/S0717345818300423 |
| _version_ | 1818345453749534720 |
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| author | Halina Wiśniewska Maciej Majka Michał Kwiatek Magdalena Gawłowska Maria Surma Tadeusz Adamski Zygmunt Kaczmarek Tadeusz Drzazga Bogusława Ługowska Marek Korbas Jolanta Belter |
| author_facet | Halina Wiśniewska Maciej Majka Michał Kwiatek Magdalena Gawłowska Maria Surma Tadeusz Adamski Zygmunt Kaczmarek Tadeusz Drzazga Bogusława Ługowska Marek Korbas Jolanta Belter |
| author_sort | Halina Wiśniewska |
| collection | DOAJ |
| description | Background: Wheat is one of the most important crops cultivated all over the world. New high-yielding cultivars that are more resistant to fungal diseases have been permanently developed. The present study aimed at the possibility of accelerating the process of breeding new cultivars, resistant to eyespot, by using doubled haploids (DH) system supported by marker-assisted selection. Results: Two highly resistant breeding lines (KBP 0916 and KBH 4942/05) carrying Pch1 gene were crossed with the elite wheat genotypes. Hybrid plants of early generations were analyzed using endopeptidase EpD1 and two SSR markers linked to the Pch1 locus. Selected homozygous and heterozygous genotypes for the Pch1-linked EpD1b allele were used to produce haploid plants. Molecular analyses were performed on haploids to identify plants possessing Pch1 gene. Chromosome doubling was performed only on haploid plants with Pch1 gene. Finally, 65 DH lines carrying eyespot resistance gene Pch1 and 30 lines without this gene were chosen for the eyespot resistance phenotyping in a field experiment. Conclusions: Results of the experiment confirmed higher resistance to eyespot of the genotypes with Pch1 in comparison to those without this gene. This indicates the efficiency of selection at the haploid level.How to cite: Wiśniewska H, Majka M, Kwiatek M, et al. Production of wheat doubled haploids resistant to eyespot supported by marker-assisted selection. Electron J Biotechnol 2019;37. https://doi.org/10.1016/j.ejbt.2018.10.003 Keywords: Breeding, Crops, Doubled haploids, Eyespot, Gene expression, Marker-assisted selection, Molecular breeding, Triticum aestivum, Wheat cultivars, Wheat genotypes, Wheat |
| first_indexed | 2024-12-13T17:02:37Z |
| format | Article |
| id | doaj.art-bfe4363f0a114e5eb8d3cc15b2f3c81a |
| institution | Directory Open Access Journal |
| issn | 0717-3458 |
| language | English |
| last_indexed | 2024-12-13T17:02:37Z |
| publishDate | 2019-01-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Electronic Journal of Biotechnology |
| spelling | doaj.art-bfe4363f0a114e5eb8d3cc15b2f3c81a2022-12-21T23:37:46ZengElsevierElectronic Journal of Biotechnology0717-34582019-01-01371117Production of wheat-doubled haploids resistant to eyespot supported by marker-assisted selectionHalina Wiśniewska0Maciej Majka1Michał Kwiatek2Magdalena Gawłowska3Maria Surma4Tadeusz Adamski5Zygmunt Kaczmarek6Tadeusz Drzazga7Bogusława Ługowska8Marek Korbas9Jolanta Belter10Department of Genomics, Institute of Plant Genetics, Polish Academy of Sciences, Strzeszyńska 34, 60-479 Poznań, Poland; Corresponding author.Department of Genomics, Institute of Plant Genetics, Polish Academy of Sciences, Strzeszyńska 34, 60-479 Poznań, PolandDepartment of Genomics, Institute of Plant Genetics, Polish Academy of Sciences, Strzeszyńska 34, 60-479 Poznań, PolandDepartment of Genomics, Institute of Plant Genetics, Polish Academy of Sciences, Strzeszyńska 34, 60-479 Poznań, PolandDepartment of Biotechnology, Institute of Plant Genetics, Polish Academy of Sciences, Strzeszyńska 34, 60-479 Poznań, PolandDepartment of Biotechnology, Institute of Plant Genetics, Polish Academy of Sciences, Strzeszyńska 34, 60-479 Poznań, PolandDepartment of Biometry and Bioinformatics, Institute of Plant Genetics, Polish Academy of Sciences, Strzeszyńska 34, 60-479 Poznań, PolandMałopolska Plant Breeding Ltd., Zbożowa 4, 30-002 Kraków, PolandDanko Plant Breeding Ltd., Choryń 27, 64-000 Kościan, PolandInstitute of Plant Protection, National Research Institute, Władysława Węgorka 20, 60-318 Poznań, PolandDepartment of Genomics, Institute of Plant Genetics, Polish Academy of Sciences, Strzeszyńska 34, 60-479 Poznań, PolandBackground: Wheat is one of the most important crops cultivated all over the world. New high-yielding cultivars that are more resistant to fungal diseases have been permanently developed. The present study aimed at the possibility of accelerating the process of breeding new cultivars, resistant to eyespot, by using doubled haploids (DH) system supported by marker-assisted selection. Results: Two highly resistant breeding lines (KBP 0916 and KBH 4942/05) carrying Pch1 gene were crossed with the elite wheat genotypes. Hybrid plants of early generations were analyzed using endopeptidase EpD1 and two SSR markers linked to the Pch1 locus. Selected homozygous and heterozygous genotypes for the Pch1-linked EpD1b allele were used to produce haploid plants. Molecular analyses were performed on haploids to identify plants possessing Pch1 gene. Chromosome doubling was performed only on haploid plants with Pch1 gene. Finally, 65 DH lines carrying eyespot resistance gene Pch1 and 30 lines without this gene were chosen for the eyespot resistance phenotyping in a field experiment. Conclusions: Results of the experiment confirmed higher resistance to eyespot of the genotypes with Pch1 in comparison to those without this gene. This indicates the efficiency of selection at the haploid level.How to cite: Wiśniewska H, Majka M, Kwiatek M, et al. Production of wheat doubled haploids resistant to eyespot supported by marker-assisted selection. Electron J Biotechnol 2019;37. https://doi.org/10.1016/j.ejbt.2018.10.003 Keywords: Breeding, Crops, Doubled haploids, Eyespot, Gene expression, Marker-assisted selection, Molecular breeding, Triticum aestivum, Wheat cultivars, Wheat genotypes, Wheathttp://www.sciencedirect.com/science/article/pii/S0717345818300423 |
| spellingShingle | Halina Wiśniewska Maciej Majka Michał Kwiatek Magdalena Gawłowska Maria Surma Tadeusz Adamski Zygmunt Kaczmarek Tadeusz Drzazga Bogusława Ługowska Marek Korbas Jolanta Belter Production of wheat-doubled haploids resistant to eyespot supported by marker-assisted selection Electronic Journal of Biotechnology |
| title | Production of wheat-doubled haploids resistant to eyespot supported by marker-assisted selection |
| title_full | Production of wheat-doubled haploids resistant to eyespot supported by marker-assisted selection |
| title_fullStr | Production of wheat-doubled haploids resistant to eyespot supported by marker-assisted selection |
| title_full_unstemmed | Production of wheat-doubled haploids resistant to eyespot supported by marker-assisted selection |
| title_short | Production of wheat-doubled haploids resistant to eyespot supported by marker-assisted selection |
| title_sort | production of wheat doubled haploids resistant to eyespot supported by marker assisted selection |
| url | http://www.sciencedirect.com/science/article/pii/S0717345818300423 |
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