Bio-Priming of Soybean with <i>Bradyrhizobium japonicum</i> and <i>Bacillus megaterium</i>: Strategy to Improve Seed Germination and the Initial Seedling Growth
Bio-priming is a new technique of seed treatment that improves seed germination, vigor, crop growth and yield. The objective of this study was to evaluate the effectiveness of <i>Bradyrhizobium japonicum</i> (commercial strains) and <i>Bacillus megaterium</i> (newly isolated...
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MDPI AG
2022-07-01
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| Online Access: | https://www.mdpi.com/2223-7747/11/15/1927 |
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| author | Dragana Miljaković Jelena Marinković Gordana Tamindžić Vuk Đorđević Branislava Tintor Dragana Milošević Maja Ignjatov Zorica Nikolić |
| author_facet | Dragana Miljaković Jelena Marinković Gordana Tamindžić Vuk Đorđević Branislava Tintor Dragana Milošević Maja Ignjatov Zorica Nikolić |
| author_sort | Dragana Miljaković |
| collection | DOAJ |
| description | Bio-priming is a new technique of seed treatment that improves seed germination, vigor, crop growth and yield. The objective of this study was to evaluate the effectiveness of <i>Bradyrhizobium japonicum</i> (commercial strains) and <i>Bacillus megaterium</i> (newly isolated strains) as a single inoculant and co-inoculant during seed bio-priming to improve seed germination and initial seedling growth of two soybean cultivars. The treated seeds were subjected to germination test (GT), cold test (CT) and accelerated aging test (AAT). <i>B. megaterium</i> significantly improved all parameters in GT and CT; final germination, shoot length, root length, root dry weight, and seedling vigor index in AAT, as compared to control. In addition, co-inoculation significantly increased all parameters except shoot dry weight in GT; all parameters in CT; germination energy, shoot length, root length, and seedling vigor index in AAT, in comparison to the control. Moreover, <i>Br. japonicum</i> significantly improved the germination energy, shoot length, shoot dry weight, root dry weight, and seedling vigor index in GT; all parameters in CT; shoot length, root length, and seedling vigor index in AAT, compared with non-primed seeds. Thus, <i>B. megaterium</i> strains could be used in soybean bio-priming as a potential single inoculant and co-inoculant, following proper field evaluation. |
| first_indexed | 2024-03-09T05:06:19Z |
| format | Article |
| id | doaj.art-bfe57daccf10479db2f4ca51e632be27 |
| institution | Directory Open Access Journal |
| issn | 2223-7747 |
| language | English |
| last_indexed | 2024-03-09T05:06:19Z |
| publishDate | 2022-07-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Plants |
| spelling | doaj.art-bfe57daccf10479db2f4ca51e632be272023-12-03T12:54:49ZengMDPI AGPlants2223-77472022-07-011115192710.3390/plants11151927Bio-Priming of Soybean with <i>Bradyrhizobium japonicum</i> and <i>Bacillus megaterium</i>: Strategy to Improve Seed Germination and the Initial Seedling GrowthDragana Miljaković0Jelena Marinković1Gordana Tamindžić2Vuk Đorđević3Branislava Tintor4Dragana Milošević5Maja Ignjatov6Zorica Nikolić7Department of Microbiological Preparations, Institute of Field and Vegetable Crops, 21000 Novi Sad, SerbiaDepartment of Microbiological Preparations, Institute of Field and Vegetable Crops, 21000 Novi Sad, SerbiaLaboratory for Seed Testing, Institute of Field and Vegetable Crops, 21000 Novi Sad, SerbiaDepartment of Legumes, Institute of Field and Vegetable Crops, 21000 Novi Sad, SerbiaDepartment of Microbiological Preparations, Institute of Field and Vegetable Crops, 21000 Novi Sad, SerbiaLaboratory for Seed Testing, Institute of Field and Vegetable Crops, 21000 Novi Sad, SerbiaDepartment of Vegetable and Alternative Crops, Institute of Field and Vegetable Crops, 21000 Novi Sad, SerbiaLaboratory for Seed Testing, Institute of Field and Vegetable Crops, 21000 Novi Sad, SerbiaBio-priming is a new technique of seed treatment that improves seed germination, vigor, crop growth and yield. The objective of this study was to evaluate the effectiveness of <i>Bradyrhizobium japonicum</i> (commercial strains) and <i>Bacillus megaterium</i> (newly isolated strains) as a single inoculant and co-inoculant during seed bio-priming to improve seed germination and initial seedling growth of two soybean cultivars. The treated seeds were subjected to germination test (GT), cold test (CT) and accelerated aging test (AAT). <i>B. megaterium</i> significantly improved all parameters in GT and CT; final germination, shoot length, root length, root dry weight, and seedling vigor index in AAT, as compared to control. In addition, co-inoculation significantly increased all parameters except shoot dry weight in GT; all parameters in CT; germination energy, shoot length, root length, and seedling vigor index in AAT, in comparison to the control. Moreover, <i>Br. japonicum</i> significantly improved the germination energy, shoot length, shoot dry weight, root dry weight, and seedling vigor index in GT; all parameters in CT; shoot length, root length, and seedling vigor index in AAT, compared with non-primed seeds. Thus, <i>B. megaterium</i> strains could be used in soybean bio-priming as a potential single inoculant and co-inoculant, following proper field evaluation.https://www.mdpi.com/2223-7747/11/15/1927bio-primingseed and seedling qualitysoybeanstressPGPR |
| spellingShingle | Dragana Miljaković Jelena Marinković Gordana Tamindžić Vuk Đorđević Branislava Tintor Dragana Milošević Maja Ignjatov Zorica Nikolić Bio-Priming of Soybean with <i>Bradyrhizobium japonicum</i> and <i>Bacillus megaterium</i>: Strategy to Improve Seed Germination and the Initial Seedling Growth Plants bio-priming seed and seedling quality soybean stress PGPR |
| title | Bio-Priming of Soybean with <i>Bradyrhizobium japonicum</i> and <i>Bacillus megaterium</i>: Strategy to Improve Seed Germination and the Initial Seedling Growth |
| title_full | Bio-Priming of Soybean with <i>Bradyrhizobium japonicum</i> and <i>Bacillus megaterium</i>: Strategy to Improve Seed Germination and the Initial Seedling Growth |
| title_fullStr | Bio-Priming of Soybean with <i>Bradyrhizobium japonicum</i> and <i>Bacillus megaterium</i>: Strategy to Improve Seed Germination and the Initial Seedling Growth |
| title_full_unstemmed | Bio-Priming of Soybean with <i>Bradyrhizobium japonicum</i> and <i>Bacillus megaterium</i>: Strategy to Improve Seed Germination and the Initial Seedling Growth |
| title_short | Bio-Priming of Soybean with <i>Bradyrhizobium japonicum</i> and <i>Bacillus megaterium</i>: Strategy to Improve Seed Germination and the Initial Seedling Growth |
| title_sort | bio priming of soybean with i bradyrhizobium japonicum i and i bacillus megaterium i strategy to improve seed germination and the initial seedling growth |
| topic | bio-priming seed and seedling quality soybean stress PGPR |
| url | https://www.mdpi.com/2223-7747/11/15/1927 |
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