Authentication of <i>Greek PDO Kalamata</i> Table Olives: A Novel Non-Target High Resolution Mass Spectrometric Approach
Food science continually requires the development of novel analytical methods to prevent fraudulent actions and guarantee food authenticity. Greek table olives, one of the most emblematic and valuable Greek national products, are often subjected to economically motivated fraud. In this work, a novel...
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| Format: | Article |
| Language: | English |
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MDPI AG
2020-06-01
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| Series: | Molecules |
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| Online Access: | https://www.mdpi.com/1420-3049/25/12/2919 |
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| author | Natasa P. Kalogiouri Reza Aalizadeh Marilena E. Dasenaki Nikolaos S. Thomaidis |
| author_facet | Natasa P. Kalogiouri Reza Aalizadeh Marilena E. Dasenaki Nikolaos S. Thomaidis |
| author_sort | Natasa P. Kalogiouri |
| collection | DOAJ |
| description | Food science continually requires the development of novel analytical methods to prevent fraudulent actions and guarantee food authenticity. Greek table olives, one of the most emblematic and valuable Greek national products, are often subjected to economically motivated fraud. In this work, a novel ultra-high-performance liquid chromatography–quadrupole time of flight tandem mass spectrometry (UHPLC-QTOF-MS) analytical method was developed to detect the mislabeling of <i>Greek PDO Kalamata table olives</i>, and thereby establish their authenticity. A non-targeted screening workflow was applied, coupled to advanced chemometric techniques such as Principal Component Analysis (PCA) and Partial Least Square Discriminant Analysis (PLS-DA) in order to fingerprint and accurately discriminate <i>PDO</i> Greek <i>Kalamata</i> olives from <i>Kalamata (or Kalamon) type</i> olives from Egypt and Chile. The method performance was evaluated using a target set of phenolic compounds and several validation parameters were calculated. Overall, 65 table olive samples from Greece, Egypt, and Chile were analyzed and processed for the model development and its accuracy was validated. The robustness of the chemometric model was tested using 11 Greek <i>Kalamon</i> olive samples that were produced during the following crop year, 2018, and they were successfully classified as Greek <i>Kalamon</i> olives from Kalamata. Twenty-six characteristic authenticity markers were indicated to be responsible for the discrimination of <i>Kalamon</i> olives of different geographical origins. |
| first_indexed | 2024-03-10T18:54:37Z |
| format | Article |
| id | doaj.art-bfe967baa6db44bbb53616f0644f0c4a |
| institution | Directory Open Access Journal |
| issn | 1420-3049 |
| language | English |
| last_indexed | 2024-03-10T18:54:37Z |
| publishDate | 2020-06-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Molecules |
| spelling | doaj.art-bfe967baa6db44bbb53616f0644f0c4a2023-11-20T04:53:46ZengMDPI AGMolecules1420-30492020-06-012512291910.3390/molecules25122919Authentication of <i>Greek PDO Kalamata</i> Table Olives: A Novel Non-Target High Resolution Mass Spectrometric ApproachNatasa P. Kalogiouri0Reza Aalizadeh1Marilena E. Dasenaki2Nikolaos S. Thomaidis3Laboratory of Analytical Chemistry, Department of Chemistry, National and Kapodistrian University of Athens, Panepistimiopolis Zographou, 15771 Athens, GreeceLaboratory of Analytical Chemistry, Department of Chemistry, National and Kapodistrian University of Athens, Panepistimiopolis Zographou, 15771 Athens, GreeceLaboratory of Analytical Chemistry, Department of Chemistry, National and Kapodistrian University of Athens, Panepistimiopolis Zographou, 15771 Athens, GreeceLaboratory of Analytical Chemistry, Department of Chemistry, National and Kapodistrian University of Athens, Panepistimiopolis Zographou, 15771 Athens, GreeceFood science continually requires the development of novel analytical methods to prevent fraudulent actions and guarantee food authenticity. Greek table olives, one of the most emblematic and valuable Greek national products, are often subjected to economically motivated fraud. In this work, a novel ultra-high-performance liquid chromatography–quadrupole time of flight tandem mass spectrometry (UHPLC-QTOF-MS) analytical method was developed to detect the mislabeling of <i>Greek PDO Kalamata table olives</i>, and thereby establish their authenticity. A non-targeted screening workflow was applied, coupled to advanced chemometric techniques such as Principal Component Analysis (PCA) and Partial Least Square Discriminant Analysis (PLS-DA) in order to fingerprint and accurately discriminate <i>PDO</i> Greek <i>Kalamata</i> olives from <i>Kalamata (or Kalamon) type</i> olives from Egypt and Chile. The method performance was evaluated using a target set of phenolic compounds and several validation parameters were calculated. Overall, 65 table olive samples from Greece, Egypt, and Chile were analyzed and processed for the model development and its accuracy was validated. The robustness of the chemometric model was tested using 11 Greek <i>Kalamon</i> olive samples that were produced during the following crop year, 2018, and they were successfully classified as Greek <i>Kalamon</i> olives from Kalamata. Twenty-six characteristic authenticity markers were indicated to be responsible for the discrimination of <i>Kalamon</i> olives of different geographical origins.https://www.mdpi.com/1420-3049/25/12/2919Kalamata olives<i>PDO</i>non-target analysismarkersQTOF-MSPLS-DA |
| spellingShingle | Natasa P. Kalogiouri Reza Aalizadeh Marilena E. Dasenaki Nikolaos S. Thomaidis Authentication of <i>Greek PDO Kalamata</i> Table Olives: A Novel Non-Target High Resolution Mass Spectrometric Approach Molecules Kalamata olives <i>PDO</i> non-target analysis markers QTOF-MS PLS-DA |
| title | Authentication of <i>Greek PDO Kalamata</i> Table Olives: A Novel Non-Target High Resolution Mass Spectrometric Approach |
| title_full | Authentication of <i>Greek PDO Kalamata</i> Table Olives: A Novel Non-Target High Resolution Mass Spectrometric Approach |
| title_fullStr | Authentication of <i>Greek PDO Kalamata</i> Table Olives: A Novel Non-Target High Resolution Mass Spectrometric Approach |
| title_full_unstemmed | Authentication of <i>Greek PDO Kalamata</i> Table Olives: A Novel Non-Target High Resolution Mass Spectrometric Approach |
| title_short | Authentication of <i>Greek PDO Kalamata</i> Table Olives: A Novel Non-Target High Resolution Mass Spectrometric Approach |
| title_sort | authentication of i greek pdo kalamata i table olives a novel non target high resolution mass spectrometric approach |
| topic | Kalamata olives <i>PDO</i> non-target analysis markers QTOF-MS PLS-DA |
| url | https://www.mdpi.com/1420-3049/25/12/2919 |
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