A Silk Fibroin Based Hydration Accelerator for Root Canal Filling Materials
Mineral trioxide aggregate (MTA) is widely used in various dental endodontic applications such as root-end filling, furcal perforation repair, and vital pulp therapy. In spite of many attempts to improve handling properties and reduce the discoloration of MTA, the ideal root canal filling material h...
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| Format: | Article |
| Language: | English |
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MDPI AG
2020-04-01
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| Series: | Polymers |
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| Online Access: | https://www.mdpi.com/2073-4360/12/4/994 |
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| author | Ching-Shuan Huang Sung-Chih Hsieh Nai-Chia Teng Wei-Fang Lee Poonam Negi Wendimi Fatimata Belem Hsuan-Chen Wu Jen-Chang Yang |
| author_facet | Ching-Shuan Huang Sung-Chih Hsieh Nai-Chia Teng Wei-Fang Lee Poonam Negi Wendimi Fatimata Belem Hsuan-Chen Wu Jen-Chang Yang |
| author_sort | Ching-Shuan Huang |
| collection | DOAJ |
| description | Mineral trioxide aggregate (MTA) is widely used in various dental endodontic applications such as root-end filling, furcal perforation repair, and vital pulp therapy. In spite of many attempts to improve handling properties and reduce the discoloration of MTA, the ideal root canal filling material has yet to be fully developed. The objective of this study was to investigate the setting time, mechanical properties, and biocompatibility of MTA set by a silk fibroin solution. A 5 wt% silk fibroin (SF) solution (a novel hydration accelerant) was used to set SavDen<sup>®</sup> MTA and ProRoot<sup>®</sup> white MTA (WMTA). Changes in setting time, diametral tensile strength (DTS), material crystallization, in vitro cell viability, and cell morphology were assessed by Vicat needle measurement, a universal testing machine, scanning electron microscopy (SEM), and WST-1 assay, respectively. The initial setting time of ProRoot<sup>®</sup> MTA and SavDen<sup>®</sup> MTA experienced a drastic decrease of 83.9% and 42.1% when deionized water was replaced by 5 wt% SF solution as the liquid phase. The DTS of SavDen<sup>®</sup> MTA showed a significant increase after set by the SF solution in 24 h. A human osteoblast-like cell (MG-63)-based WST-1 assay revealed that both ProRoot<sup>®</sup> MTA and SavDen<sup>®</sup> MTA hydrated using SF solution did not significantly differ (<i>p</i> > 0.05) in cell viability. MG-63 cells with pseudopodia attachments and nuclear protrusions represent a healthier and more adherent status on the surface of MTA when set with SF solution. The results suggest that the 5 wt% SF solution may be used as an alternative hydration accelerant for MTA in endodontic applications. |
| first_indexed | 2024-03-10T20:15:46Z |
| format | Article |
| id | doaj.art-bfec8481199f4a4f89a3ca41abddf076 |
| institution | Directory Open Access Journal |
| issn | 2073-4360 |
| language | English |
| last_indexed | 2024-03-10T20:15:46Z |
| publishDate | 2020-04-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Polymers |
| spelling | doaj.art-bfec8481199f4a4f89a3ca41abddf0762023-11-19T22:38:04ZengMDPI AGPolymers2073-43602020-04-0112499410.3390/polym12040994A Silk Fibroin Based Hydration Accelerator for Root Canal Filling MaterialsChing-Shuan Huang0Sung-Chih Hsieh1Nai-Chia Teng2Wei-Fang Lee3Poonam Negi4Wendimi Fatimata Belem5Hsuan-Chen Wu6Jen-Chang Yang7School of Dentistry, College of Oral Medicine, Taipei Medical University, Taipei 110-31, TaiwanSchool of Dentistry, College of Oral Medicine, Taipei Medical University, Taipei 110-31, TaiwanSchool of Dentistry, College of Oral Medicine, Taipei Medical University, Taipei 110-31, TaiwanSchool of Dental Technology, College of Oral Medicine, Taipei Medical University, Taipei 110, TaiwanSchool of Pharmaceutical Sciences, Shoolini University of Biotechnology and Management Sciences, Solan 173 212, IndiaInternational Ph.D. Program in Biomedical Engineering, College of Biomedical Engineering, Taipei Medical University, Taipei 110-31, TaiwanDepartment of Biochemical Science and Technology, National Taiwan University, Taipei 106, TaiwanInternational Ph.D. Program in Biomedical Engineering, College of Biomedical Engineering, Taipei Medical University, Taipei 110-31, TaiwanMineral trioxide aggregate (MTA) is widely used in various dental endodontic applications such as root-end filling, furcal perforation repair, and vital pulp therapy. In spite of many attempts to improve handling properties and reduce the discoloration of MTA, the ideal root canal filling material has yet to be fully developed. The objective of this study was to investigate the setting time, mechanical properties, and biocompatibility of MTA set by a silk fibroin solution. A 5 wt% silk fibroin (SF) solution (a novel hydration accelerant) was used to set SavDen<sup>®</sup> MTA and ProRoot<sup>®</sup> white MTA (WMTA). Changes in setting time, diametral tensile strength (DTS), material crystallization, in vitro cell viability, and cell morphology were assessed by Vicat needle measurement, a universal testing machine, scanning electron microscopy (SEM), and WST-1 assay, respectively. The initial setting time of ProRoot<sup>®</sup> MTA and SavDen<sup>®</sup> MTA experienced a drastic decrease of 83.9% and 42.1% when deionized water was replaced by 5 wt% SF solution as the liquid phase. The DTS of SavDen<sup>®</sup> MTA showed a significant increase after set by the SF solution in 24 h. A human osteoblast-like cell (MG-63)-based WST-1 assay revealed that both ProRoot<sup>®</sup> MTA and SavDen<sup>®</sup> MTA hydrated using SF solution did not significantly differ (<i>p</i> > 0.05) in cell viability. MG-63 cells with pseudopodia attachments and nuclear protrusions represent a healthier and more adherent status on the surface of MTA when set with SF solution. The results suggest that the 5 wt% SF solution may be used as an alternative hydration accelerant for MTA in endodontic applications.https://www.mdpi.com/2073-4360/12/4/994mineral trioxide aggregatesilk fibroinSavDen<sup>®</sup> MTAProRoot<sup>®</sup> MTA |
| spellingShingle | Ching-Shuan Huang Sung-Chih Hsieh Nai-Chia Teng Wei-Fang Lee Poonam Negi Wendimi Fatimata Belem Hsuan-Chen Wu Jen-Chang Yang A Silk Fibroin Based Hydration Accelerator for Root Canal Filling Materials Polymers mineral trioxide aggregate silk fibroin SavDen<sup>®</sup> MTA ProRoot<sup>®</sup> MTA |
| title | A Silk Fibroin Based Hydration Accelerator for Root Canal Filling Materials |
| title_full | A Silk Fibroin Based Hydration Accelerator for Root Canal Filling Materials |
| title_fullStr | A Silk Fibroin Based Hydration Accelerator for Root Canal Filling Materials |
| title_full_unstemmed | A Silk Fibroin Based Hydration Accelerator for Root Canal Filling Materials |
| title_short | A Silk Fibroin Based Hydration Accelerator for Root Canal Filling Materials |
| title_sort | silk fibroin based hydration accelerator for root canal filling materials |
| topic | mineral trioxide aggregate silk fibroin SavDen<sup>®</sup> MTA ProRoot<sup>®</sup> MTA |
| url | https://www.mdpi.com/2073-4360/12/4/994 |
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