Canine visceral leishmaniasis in Rondônia, Brazil: a report of an autochthonous case
Visceral Leishmaniasis (VL), also known as kala-azar, is a disseminated protozoan infection caused by the Leishmania donovani complex. Traditionally, the definitive diagnosis is made by detecting amastigotes in biological tissue samples. In August 2015, an apparently healthy, young adult, female, mo...
| Main Authors: | , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Universidade Federal Rural do Semi-Árido (UFERSA)
2021-04-01
|
| Series: | Acta Veterinaria Brasilica |
| Subjects: | |
| Online Access: | https://periodicos.ufersa.edu.br/index.php/acta/article/view/9422 |
| _version_ | 1797766677162622976 |
|---|---|
| author | Henrique Momo Ziemniczak Guilherme Henrique Lemes da Silva Maerle Oliveira Maia Elvino Ferreira Klaus Casaro Saturnino Thaís Rabelo dos Santos-Doni |
| author_facet | Henrique Momo Ziemniczak Guilherme Henrique Lemes da Silva Maerle Oliveira Maia Elvino Ferreira Klaus Casaro Saturnino Thaís Rabelo dos Santos-Doni |
| author_sort | Henrique Momo Ziemniczak |
| collection | DOAJ |
| description | Visceral Leishmaniasis (VL), also known as kala-azar, is a disseminated protozoan infection caused by the Leishmania donovani complex. Traditionally, the definitive diagnosis is made by detecting amastigotes in biological tissue samples. In August 2015, an apparently healthy, young adult, female, mongrel dog from the Zoonosis Control Center of the Rolim de Moura, Rondônia, was subjected to the canine visceral leishmaniasis diagnosis. The diagnosis was conducted using bone marrow aspirate smears and popliteal lymph node smears by Fine-needle aspiration biopsy (FNAB); stained slides with Diff-Quick screened for Leishmania amastigotes by means of direct optical microscopic examination (100×). Lymph node and bone marrow aspirates were used to investigate the presence of Leishmania infantum chagasi DNA by real-time PCR. The popliteal lymph node positive result was observed in typical amastigotes, presenting nucleus and kinetoplasts associated with lymphoblastic proliferation, reactive macrophages, plasmocytes (commonly Mott cells), eosinophils, and lymphoglandular hyperplasia. A positive bone marrow sample resulted from the observation of amastigotes in the monocyte cytoplasm or free on the smear background. The presence of the L. infantum kDNA was detected in lymph node and bone marrow FNAB samples. This research note describes the autochthonous case of CVL recorded in the state of Rondônia, Brazil, a nonendemic area for VL. According to the data, future studies must include a larger number of animals to elucidate the parasite's epidemiological resource in Rondônia. |
| first_indexed | 2024-03-12T20:28:36Z |
| format | Article |
| id | doaj.art-bfedfed7389d4a30a6c4b15307453ccf |
| institution | Directory Open Access Journal |
| issn | 1981-5484 |
| language | English |
| last_indexed | 2024-03-12T20:28:36Z |
| publishDate | 2021-04-01 |
| publisher | Universidade Federal Rural do Semi-Árido (UFERSA) |
| record_format | Article |
| series | Acta Veterinaria Brasilica |
| spelling | doaj.art-bfedfed7389d4a30a6c4b15307453ccf2023-08-02T00:20:14ZengUniversidade Federal Rural do Semi-Árido (UFERSA)Acta Veterinaria Brasilica1981-54842021-04-01151151810.21708/avb.2021.15.1.9422Canine visceral leishmaniasis in Rondônia, Brazil: a report of an autochthonous caseHenrique Momo Ziemniczak0https://orcid.org/0000-0002-7198-8939Guilherme Henrique Lemes da Silva1https://orcid.org/0000-0002-0691-6031Maerle Oliveira Maia2https://orcid.org/0000-0003-3770-6990Elvino Ferreira3https://orcid.org/0000-0001-9174-8468Klaus Casaro Saturnino4https://orcid.org/0000-0001-8493-8669Thaís Rabelo dos Santos-Doni5https://orcid.org/0000-0002-8300-0473Department of Veterinary Medicine, Rondônia Federal University - Rolim de Moura (RO), Brazil.Department of Veterinary Medicine, Rondônia Federal University - Rolim de Moura (RO), Brazil.Department of Veterinary Medicine, Rondônia Federal University - Rolim de Moura (RO), Brazil.Department of Veterinary Medicine, Rondônia Federal University - Rolim de Moura (RO), Brazil.Federal University of Goiás - Jataí (GO), Brazil.Federal University of Jequitinhonha and Mucuri Valleys - Unaí (MG), Brazil.Visceral Leishmaniasis (VL), also known as kala-azar, is a disseminated protozoan infection caused by the Leishmania donovani complex. Traditionally, the definitive diagnosis is made by detecting amastigotes in biological tissue samples. In August 2015, an apparently healthy, young adult, female, mongrel dog from the Zoonosis Control Center of the Rolim de Moura, Rondônia, was subjected to the canine visceral leishmaniasis diagnosis. The diagnosis was conducted using bone marrow aspirate smears and popliteal lymph node smears by Fine-needle aspiration biopsy (FNAB); stained slides with Diff-Quick screened for Leishmania amastigotes by means of direct optical microscopic examination (100×). Lymph node and bone marrow aspirates were used to investigate the presence of Leishmania infantum chagasi DNA by real-time PCR. The popliteal lymph node positive result was observed in typical amastigotes, presenting nucleus and kinetoplasts associated with lymphoblastic proliferation, reactive macrophages, plasmocytes (commonly Mott cells), eosinophils, and lymphoglandular hyperplasia. A positive bone marrow sample resulted from the observation of amastigotes in the monocyte cytoplasm or free on the smear background. The presence of the L. infantum kDNA was detected in lymph node and bone marrow FNAB samples. This research note describes the autochthonous case of CVL recorded in the state of Rondônia, Brazil, a nonendemic area for VL. According to the data, future studies must include a larger number of animals to elucidate the parasite's epidemiological resource in Rondônia.https://periodicos.ufersa.edu.br/index.php/acta/article/view/9422leishmaniacytologypcrlymph nodedog |
| spellingShingle | Henrique Momo Ziemniczak Guilherme Henrique Lemes da Silva Maerle Oliveira Maia Elvino Ferreira Klaus Casaro Saturnino Thaís Rabelo dos Santos-Doni Canine visceral leishmaniasis in Rondônia, Brazil: a report of an autochthonous case Acta Veterinaria Brasilica leishmania cytology pcr lymph node dog |
| title | Canine visceral leishmaniasis in Rondônia, Brazil: a report of an autochthonous case |
| title_full | Canine visceral leishmaniasis in Rondônia, Brazil: a report of an autochthonous case |
| title_fullStr | Canine visceral leishmaniasis in Rondônia, Brazil: a report of an autochthonous case |
| title_full_unstemmed | Canine visceral leishmaniasis in Rondônia, Brazil: a report of an autochthonous case |
| title_short | Canine visceral leishmaniasis in Rondônia, Brazil: a report of an autochthonous case |
| title_sort | canine visceral leishmaniasis in rondonia brazil a report of an autochthonous case |
| topic | leishmania cytology pcr lymph node dog |
| url | https://periodicos.ufersa.edu.br/index.php/acta/article/view/9422 |
| work_keys_str_mv | AT henriquemomoziemniczak caninevisceralleishmaniasisinrondoniabrazilareportofanautochthonouscase AT guilhermehenriquelemesdasilva caninevisceralleishmaniasisinrondoniabrazilareportofanautochthonouscase AT maerleoliveiramaia caninevisceralleishmaniasisinrondoniabrazilareportofanautochthonouscase AT elvinoferreira caninevisceralleishmaniasisinrondoniabrazilareportofanautochthonouscase AT klauscasarosaturnino caninevisceralleishmaniasisinrondoniabrazilareportofanautochthonouscase AT thaisrabelodossantosdoni caninevisceralleishmaniasisinrondoniabrazilareportofanautochthonouscase |