Comparison between cigarette smoke-induced emphysema and cigarette smoke extract-induced emphysema
Background Emphysema is the main pathological feature of COPD and also is the focus of the related research. Although several emphysema animal models have been established, exact comparison of findings is seldom. The present study aimed to compare cigarette smoke (CS) exposure-induced emphysema mode...
| Main Authors: | , , , , , , |
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| Format: | Article |
| Language: | English |
| Published: |
European Publishing
2015-03-01
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| Series: | Tobacco Induced Diseases |
| Subjects: | |
| Online Access: | http://www.journalssystem.com/tid/Comparison-between-cigarette-smoke-induced-emphysema-and-cigarette-smoke-extract-induced-emphysema,67144,0,2.html |
| _version_ | 1818568911094808576 |
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| author | Zhi-Hui He Ping Chen Yan Chen Sheng-Dong He Ji-Ru Ye Hong-Liang Zhang Jun Cao |
| author_facet | Zhi-Hui He Ping Chen Yan Chen Sheng-Dong He Ji-Ru Ye Hong-Liang Zhang Jun Cao |
| author_sort | Zhi-Hui He |
| collection | DOAJ |
| description | Background
Emphysema is the main pathological feature of COPD and also is the focus of the related research. Although several emphysema animal models have been established, exact comparison of findings is seldom. The present study aimed to compare cigarette smoke (CS) exposure-induced emphysema model and intraperitoneal injection of cigarette smoke extract (CSE)-induced emphysema model to evaluate the effectiveness of the two different modeling methods.
Methods
Six-week-old male C57BL/6 J mice were used and randomly divided into two groups: CS exposure and intraperitoneal injection of CSE. Each group was subdivided into two subgroups: control and CS or CSE. Lung function, mean linear intercept (MLI), destructive index (DI), apoptotic index (AI), total and differential cells count in broncholavolar lavage fluid (BALF), SOD and IL-6 concentration in serum were measured.
Results
Compared with their respective controls, lung function was significantly decreased in CS and CSE groups (P < 0.01); MLI, DI, and AI of lung tissue were significantly higher in CS and CSE groups (P < 0.01); total number of leukocytes, the number and percentage of neutrophils (NEUs), and the number of macrophages (MAC) in BALF were significantly higher in CS and CSE groups (P < 0.01); SOD concentration in serum was significantly decreased in CS and CSE groups (P < 0.01); IL-6 concentration in serum was significantly increased in in CS and CSE groups (P < 0.01). There was no significant difference between CS group and CSE group in any of the parameters described above.
Conclusions
Both CS exposure and intraperitoneal injection of CSE could induce emphysema and the effectiveness of the two different modeling methods were equal. |
| first_indexed | 2024-12-14T06:41:15Z |
| format | Article |
| id | doaj.art-bfee6b169e6f4dd4a9bc8505caf0945a |
| institution | Directory Open Access Journal |
| issn | 1617-9625 |
| language | English |
| last_indexed | 2024-12-14T06:41:15Z |
| publishDate | 2015-03-01 |
| publisher | European Publishing |
| record_format | Article |
| series | Tobacco Induced Diseases |
| spelling | doaj.art-bfee6b169e6f4dd4a9bc8505caf0945a2022-12-21T23:13:14ZengEuropean PublishingTobacco Induced Diseases1617-96252015-03-0113March10.1186/s12971-015-0033-z67144Comparison between cigarette smoke-induced emphysema and cigarette smoke extract-induced emphysemaZhi-Hui He0Ping Chen1Yan Chen2Sheng-Dong He3Ji-Ru Ye4Hong-Liang Zhang5Jun Cao6Intensive Care Unit, the Second Xiangya Hospital, Central-South University, Changsha, ChinaDepartment of Respiratory Medicine, The Second Xiangya Hospital, Central-South University, Changsha, ChinaDepartment of Respiratory Medicine, The Second Xiangya Hospital, Central-South University, Changsha, ChinaDepartment of Respiratory Medicine, The Second Xiangya Hospital, Central-South University, Changsha, ChinaDepartment of Respiratory Medicine, The Second Xiangya Hospital, Central-South University, Changsha, ChinaDepartment of Respiratory Medicine, The Second Xiangya Hospital, Central-South University, Changsha, ChinaDepartment of Respiratory Medicine, Hunan Provincial People’s Hospital, Changsha, ChinaBackground Emphysema is the main pathological feature of COPD and also is the focus of the related research. Although several emphysema animal models have been established, exact comparison of findings is seldom. The present study aimed to compare cigarette smoke (CS) exposure-induced emphysema model and intraperitoneal injection of cigarette smoke extract (CSE)-induced emphysema model to evaluate the effectiveness of the two different modeling methods. Methods Six-week-old male C57BL/6 J mice were used and randomly divided into two groups: CS exposure and intraperitoneal injection of CSE. Each group was subdivided into two subgroups: control and CS or CSE. Lung function, mean linear intercept (MLI), destructive index (DI), apoptotic index (AI), total and differential cells count in broncholavolar lavage fluid (BALF), SOD and IL-6 concentration in serum were measured. Results Compared with their respective controls, lung function was significantly decreased in CS and CSE groups (P < 0.01); MLI, DI, and AI of lung tissue were significantly higher in CS and CSE groups (P < 0.01); total number of leukocytes, the number and percentage of neutrophils (NEUs), and the number of macrophages (MAC) in BALF were significantly higher in CS and CSE groups (P < 0.01); SOD concentration in serum was significantly decreased in CS and CSE groups (P < 0.01); IL-6 concentration in serum was significantly increased in in CS and CSE groups (P < 0.01). There was no significant difference between CS group and CSE group in any of the parameters described above. Conclusions Both CS exposure and intraperitoneal injection of CSE could induce emphysema and the effectiveness of the two different modeling methods were equal.http://www.journalssystem.com/tid/Comparison-between-cigarette-smoke-induced-emphysema-and-cigarette-smoke-extract-induced-emphysema,67144,0,2.htmlanimalChronic Obstructive Pulmonary Disease (COPD)emphysemamodel |
| spellingShingle | Zhi-Hui He Ping Chen Yan Chen Sheng-Dong He Ji-Ru Ye Hong-Liang Zhang Jun Cao Comparison between cigarette smoke-induced emphysema and cigarette smoke extract-induced emphysema Tobacco Induced Diseases animal Chronic Obstructive Pulmonary Disease (COPD) emphysema model |
| title | Comparison between cigarette smoke-induced emphysema and cigarette smoke extract-induced emphysema |
| title_full | Comparison between cigarette smoke-induced emphysema and cigarette smoke extract-induced emphysema |
| title_fullStr | Comparison between cigarette smoke-induced emphysema and cigarette smoke extract-induced emphysema |
| title_full_unstemmed | Comparison between cigarette smoke-induced emphysema and cigarette smoke extract-induced emphysema |
| title_short | Comparison between cigarette smoke-induced emphysema and cigarette smoke extract-induced emphysema |
| title_sort | comparison between cigarette smoke induced emphysema and cigarette smoke extract induced emphysema |
| topic | animal Chronic Obstructive Pulmonary Disease (COPD) emphysema model |
| url | http://www.journalssystem.com/tid/Comparison-between-cigarette-smoke-induced-emphysema-and-cigarette-smoke-extract-induced-emphysema,67144,0,2.html |
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