Migration of <i>Escherichia coli</i> and <i>Klebsiella pneumoniae</i> Carbapenemase (KPC)-Producing <i>Enterobacter cloacae</i> through Wastewater Pipework and Establishment in Hospital Sink Waste Traps in a Laboratory Model System
Sink waste traps and drains are a reservoir for multi-drug resistant Gram-negative bacteria in the hospital environment. It has been suggested that these bacteria can migrate through hospital plumbing. Hospital waste traps were installed in a laboratory model system where sinks were connected throug...
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| Format: | Article |
| Language: | English |
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MDPI AG
2021-09-01
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| Series: | Microorganisms |
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| Online Access: | https://www.mdpi.com/2076-2607/9/9/1868 |
| _version_ | 1797518173333880832 |
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| author | Paz Aranega-Bou Nicholas Ellaby Matthew J. Ellington Ginny Moore |
| author_facet | Paz Aranega-Bou Nicholas Ellaby Matthew J. Ellington Ginny Moore |
| author_sort | Paz Aranega-Bou |
| collection | DOAJ |
| description | Sink waste traps and drains are a reservoir for multi-drug resistant Gram-negative bacteria in the hospital environment. It has been suggested that these bacteria can migrate through hospital plumbing. Hospital waste traps were installed in a laboratory model system where sinks were connected through a common wastewater pipe. Enterobacterales populations were monitored using selective culture, MALDI-TOF identification and antibiotic resistance profiling before and after a wastewater backflow event. When transfer between sinks was suspected, isolates were compared using whole-genome sequencing. Immediately after the wastewater backflow, two KPC-producing <i>Enterobacter cloacae</i> were recovered from a waste trap in which Carbapenemase-producing Enterobacterales (CPE) had not been detected previously. The isolates belonged to ST501 and ST31 and were genetically indistinguishable to those colonising sinks elsewhere in the system. Following inter-sink transfer, KPC-producing <i>E. cloacae</i> ST501 successfully integrated into the microbiome of the recipient sink and was detected in the waste trap water at least five months after the backflow event. Seven weeks and three months after the backflow, other inter-sink transfers involving <i>Escherichia coli</i> ST5295 and KPC-producing <i>E. cloacae</i> ST501 were also observed. |
| first_indexed | 2024-03-10T07:26:26Z |
| format | Article |
| id | doaj.art-bff289cb632349aabf77d7ef7b3925f4 |
| institution | Directory Open Access Journal |
| issn | 2076-2607 |
| language | English |
| last_indexed | 2024-03-10T07:26:26Z |
| publishDate | 2021-09-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Microorganisms |
| spelling | doaj.art-bff289cb632349aabf77d7ef7b3925f42023-11-22T14:18:19ZengMDPI AGMicroorganisms2076-26072021-09-0199186810.3390/microorganisms9091868Migration of <i>Escherichia coli</i> and <i>Klebsiella pneumoniae</i> Carbapenemase (KPC)-Producing <i>Enterobacter cloacae</i> through Wastewater Pipework and Establishment in Hospital Sink Waste Traps in a Laboratory Model SystemPaz Aranega-Bou0Nicholas Ellaby1Matthew J. Ellington2Ginny Moore3Biosafety, Air and Water Microbiology Group, National Infection Service, Public Health England, Manor Farm Rd, Porton Down, Salisbury SP4 0JG, UKAntimicrobial Resistance and Health Care Associated Infections, National infection Service, Public Health England, 61 Colindale Avenue, London NW9 5EQ, UKNational Infection Service Laboratories, Public Health England, 61 Colindale Avenue, London NW9 5EQ, UKBiosafety, Air and Water Microbiology Group, National Infection Service, Public Health England, Manor Farm Rd, Porton Down, Salisbury SP4 0JG, UKSink waste traps and drains are a reservoir for multi-drug resistant Gram-negative bacteria in the hospital environment. It has been suggested that these bacteria can migrate through hospital plumbing. Hospital waste traps were installed in a laboratory model system where sinks were connected through a common wastewater pipe. Enterobacterales populations were monitored using selective culture, MALDI-TOF identification and antibiotic resistance profiling before and after a wastewater backflow event. When transfer between sinks was suspected, isolates were compared using whole-genome sequencing. Immediately after the wastewater backflow, two KPC-producing <i>Enterobacter cloacae</i> were recovered from a waste trap in which Carbapenemase-producing Enterobacterales (CPE) had not been detected previously. The isolates belonged to ST501 and ST31 and were genetically indistinguishable to those colonising sinks elsewhere in the system. Following inter-sink transfer, KPC-producing <i>E. cloacae</i> ST501 successfully integrated into the microbiome of the recipient sink and was detected in the waste trap water at least five months after the backflow event. Seven weeks and three months after the backflow, other inter-sink transfers involving <i>Escherichia coli</i> ST5295 and KPC-producing <i>E. cloacae</i> ST501 were also observed.https://www.mdpi.com/2076-2607/9/9/1868CPEdrainshospital plumbingenvironmental contaminationinfection control |
| spellingShingle | Paz Aranega-Bou Nicholas Ellaby Matthew J. Ellington Ginny Moore Migration of <i>Escherichia coli</i> and <i>Klebsiella pneumoniae</i> Carbapenemase (KPC)-Producing <i>Enterobacter cloacae</i> through Wastewater Pipework and Establishment in Hospital Sink Waste Traps in a Laboratory Model System Microorganisms CPE drains hospital plumbing environmental contamination infection control |
| title | Migration of <i>Escherichia coli</i> and <i>Klebsiella pneumoniae</i> Carbapenemase (KPC)-Producing <i>Enterobacter cloacae</i> through Wastewater Pipework and Establishment in Hospital Sink Waste Traps in a Laboratory Model System |
| title_full | Migration of <i>Escherichia coli</i> and <i>Klebsiella pneumoniae</i> Carbapenemase (KPC)-Producing <i>Enterobacter cloacae</i> through Wastewater Pipework and Establishment in Hospital Sink Waste Traps in a Laboratory Model System |
| title_fullStr | Migration of <i>Escherichia coli</i> and <i>Klebsiella pneumoniae</i> Carbapenemase (KPC)-Producing <i>Enterobacter cloacae</i> through Wastewater Pipework and Establishment in Hospital Sink Waste Traps in a Laboratory Model System |
| title_full_unstemmed | Migration of <i>Escherichia coli</i> and <i>Klebsiella pneumoniae</i> Carbapenemase (KPC)-Producing <i>Enterobacter cloacae</i> through Wastewater Pipework and Establishment in Hospital Sink Waste Traps in a Laboratory Model System |
| title_short | Migration of <i>Escherichia coli</i> and <i>Klebsiella pneumoniae</i> Carbapenemase (KPC)-Producing <i>Enterobacter cloacae</i> through Wastewater Pipework and Establishment in Hospital Sink Waste Traps in a Laboratory Model System |
| title_sort | migration of i escherichia coli i and i klebsiella pneumoniae i carbapenemase kpc producing i enterobacter cloacae i through wastewater pipework and establishment in hospital sink waste traps in a laboratory model system |
| topic | CPE drains hospital plumbing environmental contamination infection control |
| url | https://www.mdpi.com/2076-2607/9/9/1868 |
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