Activation dynamics of antigen presenting cells in vivo against Mycobacterium bovis BCG in different immunized route
Abstract Background Control of Tuberculosis (TB) infection is mainly the result of productive teamwork between T-cell populations and antigen presenting cells (APCs). However, APCs activation at the site of initiating cellular immune response during BCG early infection is not completely understood....
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| Format: | Article |
| Language: | English |
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BMC
2023-11-01
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| Series: | BMC Immunology |
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| Online Access: | https://doi.org/10.1186/s12865-023-00589-6 |
| _version_ | 1797415641225887744 |
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| author | Zhengzhong Xu Xin Li Aihong Xia Zhifang Zhang Jiaxu Wan Yan Gao Chuang Meng Xiang Chen Xin-an Jiao |
| author_facet | Zhengzhong Xu Xin Li Aihong Xia Zhifang Zhang Jiaxu Wan Yan Gao Chuang Meng Xiang Chen Xin-an Jiao |
| author_sort | Zhengzhong Xu |
| collection | DOAJ |
| description | Abstract Background Control of Tuberculosis (TB) infection is mainly the result of productive teamwork between T-cell populations and antigen presenting cells (APCs). However, APCs activation at the site of initiating cellular immune response during BCG early infection is not completely understood. Methods In this study, we injected C57BL/6 mice in intravenous (i.v) or subcutaneous (s.c) route, then splenic or inguinal lymph node (LN) DCs and MΦs were sorted, and mycobacteria uptake, cytokine production, antigen presentation activity, and cell phenotype were investigated and compared, respectively. Results Ag85A-specific T-cell immune response began at 6 days post BCG infection, when BCG was delivered in s.c route, Th17 immune response could be induced in inguinal LN. BCG could induce high level of activation phenotype in inguinal LN MΦs, while the MHC II presentation of mycobacteria-derived peptides by DCs was more efficient than MΦs. Conclusions The results showed that BCG immunized route can decide the main tissue of T-cell immune response. Compared with s.c injected route, APCs undergo more rapid cell activation in spleen post BCG i.v infection. |
| first_indexed | 2024-03-09T05:51:32Z |
| format | Article |
| id | doaj.art-bff7caa314cd41c29f8f6a4aa106a19e |
| institution | Directory Open Access Journal |
| issn | 1471-2172 |
| language | English |
| last_indexed | 2024-03-09T05:51:32Z |
| publishDate | 2023-11-01 |
| publisher | BMC |
| record_format | Article |
| series | BMC Immunology |
| spelling | doaj.art-bff7caa314cd41c29f8f6a4aa106a19e2023-12-03T12:17:05ZengBMCBMC Immunology1471-21722023-11-0124111010.1186/s12865-023-00589-6Activation dynamics of antigen presenting cells in vivo against Mycobacterium bovis BCG in different immunized routeZhengzhong Xu0Xin Li1Aihong Xia2Zhifang Zhang3Jiaxu Wan4Yan Gao5Chuang Meng6Xiang Chen7Xin-an Jiao8Jiangsu Key Laboratory of Zoonosis/Jiangsu Co-Innovation Center for Prevention and Control of Important Animal Infectious Diseases and Zoonoses, Yangzhou UniversityKey Laboratory of Prevention and Control of Biological Hazard Factors (Animal Origin) for Agrifood Safety and Quality, Ministry of Agriculture and Rural Affairs, Yangzhou UniversityJiangsu Key Laboratory of Zoonosis/Jiangsu Co-Innovation Center for Prevention and Control of Important Animal Infectious Diseases and Zoonoses, Yangzhou UniversityJiangsu Key Laboratory of Zoonosis/Jiangsu Co-Innovation Center for Prevention and Control of Important Animal Infectious Diseases and Zoonoses, Yangzhou UniversityKey Laboratory of Prevention and Control of Biological Hazard Factors (Animal Origin) for Agrifood Safety and Quality, Ministry of Agriculture and Rural Affairs, Yangzhou UniversityKey Laboratory of Prevention and Control of Biological Hazard Factors (Animal Origin) for Agrifood Safety and Quality, Ministry of Agriculture and Rural Affairs, Yangzhou UniversityJiangsu Key Laboratory of Zoonosis/Jiangsu Co-Innovation Center for Prevention and Control of Important Animal Infectious Diseases and Zoonoses, Yangzhou UniversityJiangsu Key Laboratory of Zoonosis/Jiangsu Co-Innovation Center for Prevention and Control of Important Animal Infectious Diseases and Zoonoses, Yangzhou UniversityJiangsu Key Laboratory of Zoonosis/Jiangsu Co-Innovation Center for Prevention and Control of Important Animal Infectious Diseases and Zoonoses, Yangzhou UniversityAbstract Background Control of Tuberculosis (TB) infection is mainly the result of productive teamwork between T-cell populations and antigen presenting cells (APCs). However, APCs activation at the site of initiating cellular immune response during BCG early infection is not completely understood. Methods In this study, we injected C57BL/6 mice in intravenous (i.v) or subcutaneous (s.c) route, then splenic or inguinal lymph node (LN) DCs and MΦs were sorted, and mycobacteria uptake, cytokine production, antigen presentation activity, and cell phenotype were investigated and compared, respectively. Results Ag85A-specific T-cell immune response began at 6 days post BCG infection, when BCG was delivered in s.c route, Th17 immune response could be induced in inguinal LN. BCG could induce high level of activation phenotype in inguinal LN MΦs, while the MHC II presentation of mycobacteria-derived peptides by DCs was more efficient than MΦs. Conclusions The results showed that BCG immunized route can decide the main tissue of T-cell immune response. Compared with s.c injected route, APCs undergo more rapid cell activation in spleen post BCG i.v infection.https://doi.org/10.1186/s12865-023-00589-6M. Bovis BCGLymph nodeMacrophageCytokinesAntigen presentation |
| spellingShingle | Zhengzhong Xu Xin Li Aihong Xia Zhifang Zhang Jiaxu Wan Yan Gao Chuang Meng Xiang Chen Xin-an Jiao Activation dynamics of antigen presenting cells in vivo against Mycobacterium bovis BCG in different immunized route BMC Immunology M. Bovis BCG Lymph node Macrophage Cytokines Antigen presentation |
| title | Activation dynamics of antigen presenting cells in vivo against Mycobacterium bovis BCG in different immunized route |
| title_full | Activation dynamics of antigen presenting cells in vivo against Mycobacterium bovis BCG in different immunized route |
| title_fullStr | Activation dynamics of antigen presenting cells in vivo against Mycobacterium bovis BCG in different immunized route |
| title_full_unstemmed | Activation dynamics of antigen presenting cells in vivo against Mycobacterium bovis BCG in different immunized route |
| title_short | Activation dynamics of antigen presenting cells in vivo against Mycobacterium bovis BCG in different immunized route |
| title_sort | activation dynamics of antigen presenting cells in vivo against mycobacterium bovis bcg in different immunized route |
| topic | M. Bovis BCG Lymph node Macrophage Cytokines Antigen presentation |
| url | https://doi.org/10.1186/s12865-023-00589-6 |
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