Brucella cultures characteristics, clinical characteristics, and infection biomarkers of human Brucellosis
Objective: Aim to investigate the brucella culture characteristics, diagnosis methods, and clinical characteristics, to provide the laboratory with diagnostic methods and prevention and treatment for brucellosis. Methods: Data of 328 cases of brucellosis from 2012 to 2022 was analyzed, retrospective...
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| Format: | Article |
| Language: | English |
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Elsevier
2023-03-01
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| Series: | Journal of Infection and Public Health |
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| Online Access: | http://www.sciencedirect.com/science/article/pii/S1876034123000023 |
| _version_ | 1797903544183947264 |
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| author | Feng Qiangsheng Ha Xiaoqin Lin Tong Guo Wenyun Song Yuejuan |
| author_facet | Feng Qiangsheng Ha Xiaoqin Lin Tong Guo Wenyun Song Yuejuan |
| author_sort | Feng Qiangsheng |
| collection | DOAJ |
| description | Objective: Aim to investigate the brucella culture characteristics, diagnosis methods, and clinical characteristics, to provide the laboratory with diagnostic methods and prevention and treatment for brucellosis. Methods: Data of 328 cases of brucellosis from 2012 to 2022 was analyzed, retrospectively. The bacterial culture characteristics, the clinical diagnostic methods, and the complications were analyzed respectively. The infection biomarkers of the brucellosis were analyzed by Receiver operating characteristic curve ROC. Results: Among the 328 brucellosis, 78.96 % of cases were men, the median age of the patients was (45.21±13.49) years and the annual incidence in our region was 67/100 000 per year. The diagnostic methods included pathogenic bacteria culture, serological diagnosis, and suspect case were 24.39 %, 47.56 %, and 28.05 %, respectively, sensitivity of combined detection Standard agglutination test (SAT) and the Rose Bengal test (RBT) is 96.2 %. In our work, 80 cases of brucellosis were diagnosed by a bacterial culture which were been identified as Brucella melitensis, blood culture was the main method (78.75 %) and the average positive alarm time was 80.74 (21.6–129) h and all of them were detected in aerobic bottles, followed by synovial fluid, bone marrow, lumbar spine, and joint tissue, puncture fluid and ascites culture which were 6.25 %, 3.75 %, 5.00 %, 5.00 % and 1.25 % respectively. The brucellosis with complications was lumbar spine lesions at 41.46 % cervical spine lesions at 4.60 % and knee joint lesions at 12.8 % and another osteoarthritis. The in-hospital mortality rate of the patients was 0.91 % and all of them were meningitis patients. ROC analysis indicated CRP had high sensitivity and specificity for brucellosis, and when CRP was 1.23mg/ml, the sensitivity and specificity were 0.727 and 0.718 respectively, and the U test also indicated CRP had a significant difference, Z=5.054, p <0.001. Conclusions: Brucellosis is frequently morbidity in 40 + age men, which has been diagnosed by aerobic blood culture, generally bacterial culture, RBT and SAT, epidemiological, and commonly with complications of spine and arthropathy. |
| first_indexed | 2024-04-10T09:34:38Z |
| format | Article |
| id | doaj.art-bff9bcf22d7240349acba74781194eb7 |
| institution | Directory Open Access Journal |
| issn | 1876-0341 |
| language | English |
| last_indexed | 2024-04-10T09:34:38Z |
| publishDate | 2023-03-01 |
| publisher | Elsevier |
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| series | Journal of Infection and Public Health |
| spelling | doaj.art-bff9bcf22d7240349acba74781194eb72023-02-18T04:16:38ZengElsevierJournal of Infection and Public Health1876-03412023-03-01163303309Brucella cultures characteristics, clinical characteristics, and infection biomarkers of human BrucellosisFeng Qiangsheng0Ha Xiaoqin1Lin Tong2Guo Wenyun3Song Yuejuan4Department of Clinical Laboratory, The 940th Hospital of Joint Logistics Support Force of People’s Liberation Army, Lanzhou 730050, ChinaDepartment of Clinical Laboratory, The 940th Hospital of Joint Logistics Support Force of People’s Liberation Army, Lanzhou 730050, ChinaDepartment of Medica, The 940th Hospital of Joint Logistics Support Force of People’s Liberation Army, Lanzhou 730050, ChinaDepartment of Cardiology, The 940th Hospital of Joint Logistics Support Force of People’s Liberation Army, Lanzhou 730050, ChinaDepartment of Clinical Laboratory, The 940th Hospital of Joint Logistics Support Force of People’s Liberation Army, Lanzhou 730050, China; Corresponding author.Objective: Aim to investigate the brucella culture characteristics, diagnosis methods, and clinical characteristics, to provide the laboratory with diagnostic methods and prevention and treatment for brucellosis. Methods: Data of 328 cases of brucellosis from 2012 to 2022 was analyzed, retrospectively. The bacterial culture characteristics, the clinical diagnostic methods, and the complications were analyzed respectively. The infection biomarkers of the brucellosis were analyzed by Receiver operating characteristic curve ROC. Results: Among the 328 brucellosis, 78.96 % of cases were men, the median age of the patients was (45.21±13.49) years and the annual incidence in our region was 67/100 000 per year. The diagnostic methods included pathogenic bacteria culture, serological diagnosis, and suspect case were 24.39 %, 47.56 %, and 28.05 %, respectively, sensitivity of combined detection Standard agglutination test (SAT) and the Rose Bengal test (RBT) is 96.2 %. In our work, 80 cases of brucellosis were diagnosed by a bacterial culture which were been identified as Brucella melitensis, blood culture was the main method (78.75 %) and the average positive alarm time was 80.74 (21.6–129) h and all of them were detected in aerobic bottles, followed by synovial fluid, bone marrow, lumbar spine, and joint tissue, puncture fluid and ascites culture which were 6.25 %, 3.75 %, 5.00 %, 5.00 % and 1.25 % respectively. The brucellosis with complications was lumbar spine lesions at 41.46 % cervical spine lesions at 4.60 % and knee joint lesions at 12.8 % and another osteoarthritis. The in-hospital mortality rate of the patients was 0.91 % and all of them were meningitis patients. ROC analysis indicated CRP had high sensitivity and specificity for brucellosis, and when CRP was 1.23mg/ml, the sensitivity and specificity were 0.727 and 0.718 respectively, and the U test also indicated CRP had a significant difference, Z=5.054, p <0.001. Conclusions: Brucellosis is frequently morbidity in 40 + age men, which has been diagnosed by aerobic blood culture, generally bacterial culture, RBT and SAT, epidemiological, and commonly with complications of spine and arthropathy.http://www.sciencedirect.com/science/article/pii/S1876034123000023BrucellosisBrucella melitensisAerobic blood cultureGenerally bacterial cultureCulture characteristicsOsteoarthropathy |
| spellingShingle | Feng Qiangsheng Ha Xiaoqin Lin Tong Guo Wenyun Song Yuejuan Brucella cultures characteristics, clinical characteristics, and infection biomarkers of human Brucellosis Journal of Infection and Public Health Brucellosis Brucella melitensis Aerobic blood culture Generally bacterial culture Culture characteristics Osteoarthropathy |
| title | Brucella cultures characteristics, clinical characteristics, and infection biomarkers of human Brucellosis |
| title_full | Brucella cultures characteristics, clinical characteristics, and infection biomarkers of human Brucellosis |
| title_fullStr | Brucella cultures characteristics, clinical characteristics, and infection biomarkers of human Brucellosis |
| title_full_unstemmed | Brucella cultures characteristics, clinical characteristics, and infection biomarkers of human Brucellosis |
| title_short | Brucella cultures characteristics, clinical characteristics, and infection biomarkers of human Brucellosis |
| title_sort | brucella cultures characteristics clinical characteristics and infection biomarkers of human brucellosis |
| topic | Brucellosis Brucella melitensis Aerobic blood culture Generally bacterial culture Culture characteristics Osteoarthropathy |
| url | http://www.sciencedirect.com/science/article/pii/S1876034123000023 |
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