Multidrug resistance to antibiotics in Escherichia coli bacteria isolated from bats on Lombok Island, Indonesia
Wild animals such as bats usually do not receive antibiotic therapy, however bats can have bacteria that have developed resistance to antibiotics due to water contamination from pharmaceutical factory waste disposal, expired medicines that are thrown away carelessly, feces and urine that pollute th...
| Main Authors: | , , , , , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Assiut University
2024-04-01
|
| Series: | Journal of Advanced Veterinary Research |
| Subjects: | |
| Online Access: | https://advetresearch.com/index.php/AVR/article/view/1769 |
| _version_ | 1797224085347893248 |
|---|---|
| author | Alfiana Laili Dwi Agustin Fidi Nur Aini Eka Puji Dameanti Mustofa Helmi Effendi Wiwiek Tyasningsih Aswin Rafif Khairullah Shendy Canadya Kurniawan Ikechukwu Benjamin Moses Abdullah Hasib Yolla Rona Mustika Kurnia Nisa Kinasih |
| author_facet | Alfiana Laili Dwi Agustin Fidi Nur Aini Eka Puji Dameanti Mustofa Helmi Effendi Wiwiek Tyasningsih Aswin Rafif Khairullah Shendy Canadya Kurniawan Ikechukwu Benjamin Moses Abdullah Hasib Yolla Rona Mustika Kurnia Nisa Kinasih |
| author_sort | Alfiana Laili Dwi Agustin |
| collection | DOAJ |
| description |
Wild animals such as bats usually do not receive antibiotic therapy, however bats can have bacteria that have developed resistance to antibiotics due to water contamination from pharmaceutical factory waste disposal, expired medicines that are thrown away carelessly, feces and urine that pollute the environment. This research aimed to look at cases of multidrug resistance (MDR) in bats on the island of Lombok, Indonesia. A total of 135 bat rectal swab samples were taken using sterile cotton buds. Isolation of E. coli bacteria using Eosin Methylene Blue Agar media then looking at morphology and biochemical tests. The bacterial sensitivity test used the disk diffusion method using seven antibiotics, namely the antibiotics amoxicillin, ciprofloxacine, trimethoprim/sulfamethoxazole, tetracycline, gentamicin, cefotaxime and azithromycin. The research results showed that from 135 samples tested, 97 samples were positive for E. coli. Samples that were positive for E. coli were tested for sensitivity, showing that 41 (41/97, 42.26%) samples were still sensitive to all tested antibiotics and 56 samples experienced resistance with the results of samples experiencing resistance to the antibiotic azithromycin (AZM) as many as 40 samples ( 41.2%), amoxicillin (AML) 25 samples (25.7%), tetracycline (TE) 23 samples (23.7%), sulfamethoxazole/trimethoprim (SXT) 21 samples (21.6%), ciprofloxacine (CIP) 14 samples (14.4%), gentamicin (CN) 1 sample (1%), and for cefotaxime (CTX) 0 samples experienced resistance (0%), and 15 samples experienced multidrug resistance. These results explain that bats in caves on the Indonesian island of Lombok act as transmitters of MDR E. coli to public health.
|
| first_indexed | 2024-04-24T13:47:31Z |
| format | Article |
| id | doaj.art-bfffdc22f6944768aa526d012a1afe17 |
| institution | Directory Open Access Journal |
| issn | 2090-6269 2090-6277 |
| language | English |
| last_indexed | 2024-04-24T13:47:31Z |
| publishDate | 2024-04-01 |
| publisher | Assiut University |
| record_format | Article |
| series | Journal of Advanced Veterinary Research |
| spelling | doaj.art-bfffdc22f6944768aa526d012a1afe172024-04-04T05:13:06ZengAssiut UniversityJournal of Advanced Veterinary Research2090-62692090-62772024-04-01144Multidrug resistance to antibiotics in Escherichia coli bacteria isolated from bats on Lombok Island, IndonesiaAlfiana Laili Dwi Agustin0Fidi Nur Aini Eka Puji Dameanti1Mustofa Helmi Effendi2Wiwiek Tyasningsih3Aswin Rafif Khairullah4Shendy Canadya Kurniawan5Ikechukwu Benjamin Moses6Abdullah Hasib7Yolla Rona Mustika8Kurnia Nisa Kinasih9Doctoral Program in Veterinary Science, Faculty of Veterinary Medicine, Universitas Airlangga, Jl. Dr. Ir. H. Soekarno, Kampus C Mulyorejo, Surabaya 60115, East Java, IndonesiaMicrobiology and Immunology Laboratory, Faculty of Veterinary Medicine, Universitas Brawijaya, Jl. Veteran, Ketawanggede, Lowokwaru, Malang 65145, East Java, IndonesiaDivision of Veterinary Public Health, Faculty of Veterinary Medicine, Universitas Airlangga, Jl. Dr. Ir. H. Soekarno, Kampus C Mulyorejo, Surabaya 60115, East Java, IndonesiaDivision of Veterinary Microbiology, Faculty of Veterinary Medicine, Universitas Airlangga, Jl. Dr. Ir. H. Soekarno, Kampus C Mulyorejo, Surabaya 60115, East Java, IndonesiaDivision of Animal Husbandry, Faculty of Veterinary Medicine, Universitas Airlangga, Jl. Dr. Ir. H. Soekarno, Kampus C Mulyorejo, Surabaya 60115, East Java, IndonesiaMaster Program of Animal Sciences, Department of Animal Sciences, Specialisation in Molecule, Cell and Organ Functioning, Wageningen University and Research, Wageningen 6708 PB, NetherlandsDepartment of Applied Microbiology, Faculty of Science, Ebonyi State University, Abakaliki 480211, NigeriaSchool of Agriculture and Food Sustainability, The University of Queensland, Gatton, QLD 4343, QueenslandMaster Program in Veterinary Science and Public Health, Faculty of Veterinary Medicine, Universitas Airlangga. Jl. Dr. Ir. H. Soekarno, Kampus C Mulyorejo, Surabaya 60115, East Java, IndonesiaMaster Program in Veterinary Science and Public Health, Faculty of Veterinary Medicine, Universitas Airlangga. Jl. Dr. Ir. H. Soekarno, Kampus C Mulyorejo, Surabaya 60115, East Java, Indonesia Wild animals such as bats usually do not receive antibiotic therapy, however bats can have bacteria that have developed resistance to antibiotics due to water contamination from pharmaceutical factory waste disposal, expired medicines that are thrown away carelessly, feces and urine that pollute the environment. This research aimed to look at cases of multidrug resistance (MDR) in bats on the island of Lombok, Indonesia. A total of 135 bat rectal swab samples were taken using sterile cotton buds. Isolation of E. coli bacteria using Eosin Methylene Blue Agar media then looking at morphology and biochemical tests. The bacterial sensitivity test used the disk diffusion method using seven antibiotics, namely the antibiotics amoxicillin, ciprofloxacine, trimethoprim/sulfamethoxazole, tetracycline, gentamicin, cefotaxime and azithromycin. The research results showed that from 135 samples tested, 97 samples were positive for E. coli. Samples that were positive for E. coli were tested for sensitivity, showing that 41 (41/97, 42.26%) samples were still sensitive to all tested antibiotics and 56 samples experienced resistance with the results of samples experiencing resistance to the antibiotic azithromycin (AZM) as many as 40 samples ( 41.2%), amoxicillin (AML) 25 samples (25.7%), tetracycline (TE) 23 samples (23.7%), sulfamethoxazole/trimethoprim (SXT) 21 samples (21.6%), ciprofloxacine (CIP) 14 samples (14.4%), gentamicin (CN) 1 sample (1%), and for cefotaxime (CTX) 0 samples experienced resistance (0%), and 15 samples experienced multidrug resistance. These results explain that bats in caves on the Indonesian island of Lombok act as transmitters of MDR E. coli to public health. https://advetresearch.com/index.php/AVR/article/view/1769Batsmultidrug resistanceE. coliantibioticpublic health |
| spellingShingle | Alfiana Laili Dwi Agustin Fidi Nur Aini Eka Puji Dameanti Mustofa Helmi Effendi Wiwiek Tyasningsih Aswin Rafif Khairullah Shendy Canadya Kurniawan Ikechukwu Benjamin Moses Abdullah Hasib Yolla Rona Mustika Kurnia Nisa Kinasih Multidrug resistance to antibiotics in Escherichia coli bacteria isolated from bats on Lombok Island, Indonesia Journal of Advanced Veterinary Research Bats multidrug resistance E. coli antibiotic public health |
| title | Multidrug resistance to antibiotics in Escherichia coli bacteria isolated from bats on Lombok Island, Indonesia |
| title_full | Multidrug resistance to antibiotics in Escherichia coli bacteria isolated from bats on Lombok Island, Indonesia |
| title_fullStr | Multidrug resistance to antibiotics in Escherichia coli bacteria isolated from bats on Lombok Island, Indonesia |
| title_full_unstemmed | Multidrug resistance to antibiotics in Escherichia coli bacteria isolated from bats on Lombok Island, Indonesia |
| title_short | Multidrug resistance to antibiotics in Escherichia coli bacteria isolated from bats on Lombok Island, Indonesia |
| title_sort | multidrug resistance to antibiotics in escherichia coli bacteria isolated from bats on lombok island indonesia |
| topic | Bats multidrug resistance E. coli antibiotic public health |
| url | https://advetresearch.com/index.php/AVR/article/view/1769 |
| work_keys_str_mv | AT alfianalailidwiagustin multidrugresistancetoantibioticsinescherichiacolibacteriaisolatedfrombatsonlombokislandindonesia AT fidinurainiekapujidameanti multidrugresistancetoantibioticsinescherichiacolibacteriaisolatedfrombatsonlombokislandindonesia AT mustofahelmieffendi multidrugresistancetoantibioticsinescherichiacolibacteriaisolatedfrombatsonlombokislandindonesia AT wiwiektyasningsih multidrugresistancetoantibioticsinescherichiacolibacteriaisolatedfrombatsonlombokislandindonesia AT aswinrafifkhairullah multidrugresistancetoantibioticsinescherichiacolibacteriaisolatedfrombatsonlombokislandindonesia AT shendycanadyakurniawan multidrugresistancetoantibioticsinescherichiacolibacteriaisolatedfrombatsonlombokislandindonesia AT ikechukwubenjaminmoses multidrugresistancetoantibioticsinescherichiacolibacteriaisolatedfrombatsonlombokislandindonesia AT abdullahhasib multidrugresistancetoantibioticsinescherichiacolibacteriaisolatedfrombatsonlombokislandindonesia AT yollaronamustika multidrugresistancetoantibioticsinescherichiacolibacteriaisolatedfrombatsonlombokislandindonesia AT kurnianisakinasih multidrugresistancetoantibioticsinescherichiacolibacteriaisolatedfrombatsonlombokislandindonesia |