Seroprevalence and risk factors of avian reovirus in backyard chickens in different areas of Mymensingh district in Bangladesh
Objectives: The present study estimated the seroprevalence of avian reovirus (ARV) infections in backyard chickens of the Mymensingh district in Bangladesh. Materials and Methods: Considering several risk factors, a total of 460 serum samples were col¬lected from backyard chickens from eight Upazil...
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| Format: | Article |
| Language: | English |
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Network for the Veterinarians of Bangladesh
2020-09-01
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| Series: | Journal of Advanced Veterinary and Animal Research |
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| Online Access: | http://www.ejmanager.com/fulltextpdf.php?mno=80998 |
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| author | Md. Saiful Islam Abdullah Al Momen Sabuj Zobayda Farzana Haque Amrita Pondit Md. Golzar Hossain Sukumar Saha |
| author_facet | Md. Saiful Islam Abdullah Al Momen Sabuj Zobayda Farzana Haque Amrita Pondit Md. Golzar Hossain Sukumar Saha |
| author_sort | Md. Saiful Islam |
| collection | DOAJ |
| description | Objectives: The present study estimated the seroprevalence of avian reovirus (ARV) infections in backyard chickens of the Mymensingh district in Bangladesh.
Materials and Methods: Considering several risk factors, a total of 460 serum samples were col¬lected from backyard chickens from eight Upazilas of the Mymensingh district in Bangladesh. Blood samples were taken from the wing vein using 3-ml sterile syringes and kept at room tem¬perature for clotting in a slanting position and then transported to the laboratory maintaining the cool chain. Subsequently, the prepared sera were harvested and stored at −20°C until used. Finally, an indirect enzyme-linked immunosorbent assay (ELISA) was performed to detect ARV-specific antibodies using a commercial ARV antibody detection ELISA test kit.
Results: The results revealed high prevalence rates of ARV antibodies, with a total seroprevalence of 69.78% (321/460). Area-wise, 74.55% (82/110) seroprevalence was recorded as the highest in Mymensingh Sadar, whereas 64% (32/50) was the lowest in Gauripur Upazila. With regard to sex, female chickens showed a significantly higher (p < 0.05) seroprevalence as 90.33% (271/300) compared to male chickens 31.25% (50/160). With regard to age groups, the seroprevalence of ARV infection was 59.33% (89/150) within 28 weeks, 82% (205/250) within 916 weeks, and 45% (27/60) within 1720 weeks, respectively. Based on hygienic conditions, the highest sero¬prevalence of ARV was noted in backyard chickens housed in poor conditions 80% (120/150) than good conditions 50% (40/80). Backyard chickens reared in free-ranging conditions exhibited a significantly higher seroprevalence 73.33% (220/300) of ARV antibodies compared to rearing in separate houses 63.12% (101/160). The seroprevalence of ARV was higher in crossbreeds 71.67% (43/60), brought from market 76% (38/50), and unhealthy 78.57% (55/70) backyard chickens than non-descriptive indigenous 69.5% (278/400), home-reared 69.02% (283/410), and healthy chick¬ens 68.21% (266/390).
Conclusion: The high prevalence of ARV antibodies revealed in the current study indicates an extensive exposure of ARV to backyard chickens in Bangladesh that may be transmitted naturally to other chickens, ultimately leading to ominous economic effects on the poultry sector. [J Adv Vet Anim Res 2020; 7(3.000): 546-553] |
| first_indexed | 2024-12-13T09:28:29Z |
| format | Article |
| id | doaj.art-c00122ca29904874af14112dc933e9a9 |
| institution | Directory Open Access Journal |
| issn | 2311-7710 |
| language | English |
| last_indexed | 2024-12-13T09:28:29Z |
| publishDate | 2020-09-01 |
| publisher | Network for the Veterinarians of Bangladesh |
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| series | Journal of Advanced Veterinary and Animal Research |
| spelling | doaj.art-c00122ca29904874af14112dc933e9a92022-12-21T23:52:34ZengNetwork for the Veterinarians of BangladeshJournal of Advanced Veterinary and Animal Research2311-77102020-09-017354655310.5455/javar.2020.g45280998Seroprevalence and risk factors of avian reovirus in backyard chickens in different areas of Mymensingh district in BangladeshMd. Saiful Islam0Abdullah Al Momen Sabuj1Zobayda Farzana Haque2Amrita Pondit3Md. Golzar Hossain4Sukumar Saha5Department of Microbiology and Hygiene, Faculty of Veterinary Science, Bangladesh Agricultural University, Mymensingh-2202, Bangladesh Department of Microbiology and Hygiene, Faculty of Veterinary Science, Bangladesh Agricultural University, Mymensingh-2202, Bangladesh Department of Microbiology and Hygiene, Faculty of Veterinary Science, Bangladesh Agricultural University, Mymensingh-2202, Bangladesh Department of Microbiology and Hygiene, Faculty of Veterinary Science, Bangladesh Agricultural University, Mymensingh-2202, Bangladesh Department of Microbiology and Hygiene, Faculty of Veterinary Science, Bangladesh Agricultural University, Mymensingh-2202, Bangladesh Department of Microbiology and Hygiene, Faculty of Veterinary Science, Bangladesh Agricultural University, Mymensingh-2202, Bangladesh.Objectives: The present study estimated the seroprevalence of avian reovirus (ARV) infections in backyard chickens of the Mymensingh district in Bangladesh. Materials and Methods: Considering several risk factors, a total of 460 serum samples were col¬lected from backyard chickens from eight Upazilas of the Mymensingh district in Bangladesh. Blood samples were taken from the wing vein using 3-ml sterile syringes and kept at room tem¬perature for clotting in a slanting position and then transported to the laboratory maintaining the cool chain. Subsequently, the prepared sera were harvested and stored at −20°C until used. Finally, an indirect enzyme-linked immunosorbent assay (ELISA) was performed to detect ARV-specific antibodies using a commercial ARV antibody detection ELISA test kit. Results: The results revealed high prevalence rates of ARV antibodies, with a total seroprevalence of 69.78% (321/460). Area-wise, 74.55% (82/110) seroprevalence was recorded as the highest in Mymensingh Sadar, whereas 64% (32/50) was the lowest in Gauripur Upazila. With regard to sex, female chickens showed a significantly higher (p < 0.05) seroprevalence as 90.33% (271/300) compared to male chickens 31.25% (50/160). With regard to age groups, the seroprevalence of ARV infection was 59.33% (89/150) within 28 weeks, 82% (205/250) within 916 weeks, and 45% (27/60) within 1720 weeks, respectively. Based on hygienic conditions, the highest sero¬prevalence of ARV was noted in backyard chickens housed in poor conditions 80% (120/150) than good conditions 50% (40/80). Backyard chickens reared in free-ranging conditions exhibited a significantly higher seroprevalence 73.33% (220/300) of ARV antibodies compared to rearing in separate houses 63.12% (101/160). The seroprevalence of ARV was higher in crossbreeds 71.67% (43/60), brought from market 76% (38/50), and unhealthy 78.57% (55/70) backyard chickens than non-descriptive indigenous 69.5% (278/400), home-reared 69.02% (283/410), and healthy chick¬ens 68.21% (266/390). Conclusion: The high prevalence of ARV antibodies revealed in the current study indicates an extensive exposure of ARV to backyard chickens in Bangladesh that may be transmitted naturally to other chickens, ultimately leading to ominous economic effects on the poultry sector. [J Adv Vet Anim Res 2020; 7(3.000): 546-553]http://www.ejmanager.com/fulltextpdf.php?mno=80998avian reovirus; backyard chickens; elisarisk factors; seroprevalence; bangladesh |
| spellingShingle | Md. Saiful Islam Abdullah Al Momen Sabuj Zobayda Farzana Haque Amrita Pondit Md. Golzar Hossain Sukumar Saha Seroprevalence and risk factors of avian reovirus in backyard chickens in different areas of Mymensingh district in Bangladesh Journal of Advanced Veterinary and Animal Research avian reovirus; backyard chickens; elisa risk factors; seroprevalence; bangladesh |
| title | Seroprevalence and risk factors of avian reovirus in backyard chickens in different areas of Mymensingh district in Bangladesh |
| title_full | Seroprevalence and risk factors of avian reovirus in backyard chickens in different areas of Mymensingh district in Bangladesh |
| title_fullStr | Seroprevalence and risk factors of avian reovirus in backyard chickens in different areas of Mymensingh district in Bangladesh |
| title_full_unstemmed | Seroprevalence and risk factors of avian reovirus in backyard chickens in different areas of Mymensingh district in Bangladesh |
| title_short | Seroprevalence and risk factors of avian reovirus in backyard chickens in different areas of Mymensingh district in Bangladesh |
| title_sort | seroprevalence and risk factors of avian reovirus in backyard chickens in different areas of mymensingh district in bangladesh |
| topic | avian reovirus; backyard chickens; elisa risk factors; seroprevalence; bangladesh |
| url | http://www.ejmanager.com/fulltextpdf.php?mno=80998 |
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