Tomato Chlorosis Virus Infection Facilitates <i>Bemisia tabaci</i> MED Reproduction by Elevating <i>Vitellogenin</i> Expression
Transmission of plant pathogenic viruses mostly relies on insect vectors. Plant virus could enhance its transmission by modulating the vector. Previously, we showed that feeding on virus infected plants can promote the reproduction of the sweet potato whitefly, <i>Bemisia tabaci</i> MED...
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| Format: | Article |
| Language: | English |
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MDPI AG
2021-01-01
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| Series: | Insects |
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| Online Access: | https://www.mdpi.com/2075-4450/12/2/101 |
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| author | Liping Huang Xiaobin Shi Jizhe Shi Zhuo Zhang Yong Fang Zhanhong Zhang Qiuyi Pan Limin Zheng Yang Gao Deyong Zhang Xinqiu Tan Yong Liu Xuguo Zhou |
| author_facet | Liping Huang Xiaobin Shi Jizhe Shi Zhuo Zhang Yong Fang Zhanhong Zhang Qiuyi Pan Limin Zheng Yang Gao Deyong Zhang Xinqiu Tan Yong Liu Xuguo Zhou |
| author_sort | Liping Huang |
| collection | DOAJ |
| description | Transmission of plant pathogenic viruses mostly relies on insect vectors. Plant virus could enhance its transmission by modulating the vector. Previously, we showed that feeding on virus infected plants can promote the reproduction of the sweet potato whitefly, <i>Bemisia tabaci</i> MED (Q biotype). In this study, using a whitefly-Tomato chlorosis virus (ToCV)-tomato system, we investigated how ToCV modulates <i>B. tabaci</i> MED reproduction to facilitate its spread. Here, we hypothesized that ToCV-infected tomato plants would increase <i>B. tabaci</i> MED fecundity via elevated <i>vitellogenin (Vg)</i> gene expression. As a result, fecundity and the relative expression of <i>B. tabaci</i> MED <i>Vg</i> was measured on ToCV-infected and uninfected tomato plants on days 4, 8, 12, 16, 20 and 24. The role of <i>Vg</i> on <i>B. tabaci</i> MED reproduction was examined in the presence and absence of ToCV using dietary RNAi. ToCV infection significantly increased <i>B. tabaci</i> MED fecundity on days 12, 16 and 20, and elevated <i>Vg</i> expression on days 8, 12 and 16. Both ovarian development and fecundity of <i>B. tabaci</i> MED were suppressed when <i>Vg</i> was silenced with or without ToCV infection. These combined results suggest that ToCV infection increases <i>B. tabaci</i> MED fecundity via elevated <i>Vg</i> expression. |
| first_indexed | 2024-03-09T03:43:47Z |
| format | Article |
| id | doaj.art-c0079fa58f104194b70fb09c991c67f7 |
| institution | Directory Open Access Journal |
| issn | 2075-4450 |
| language | English |
| last_indexed | 2024-03-09T03:43:47Z |
| publishDate | 2021-01-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Insects |
| spelling | doaj.art-c0079fa58f104194b70fb09c991c67f72023-12-03T14:37:07ZengMDPI AGInsects2075-44502021-01-0112210110.3390/insects12020101Tomato Chlorosis Virus Infection Facilitates <i>Bemisia tabaci</i> MED Reproduction by Elevating <i>Vitellogenin</i> ExpressionLiping Huang0Xiaobin Shi1Jizhe Shi2Zhuo Zhang3Yong Fang4Zhanhong Zhang5Qiuyi Pan6Limin Zheng7Yang Gao8Deyong Zhang9Xinqiu Tan10Yong Liu11Xuguo Zhou12Longping Branch Graduate School, Hunan University, Changsha 410125, ChinaLongping Branch Graduate School, Hunan University, Changsha 410125, ChinaDepartment of Entomology, University of Kentucky, Lexington, KY 40546, USAInstitute of Plant Protection, Hunan Academy of Agricultural Sciences, Changsha 410125, ChinaInstitute of Biotechnology, Hunan Academy of Agricultural Sciences, Changsha 410125, ChinaInstitute of Vegetable, Hunan Academy of Agricultural Sciences, Changsha 410125, ChinaPlant Protection and Plant Inspection Station, Changde 415000, ChinaInstitute of Plant Protection, Hunan Academy of Agricultural Sciences, Changsha 410125, ChinaInstitute of Plant Protection, Hunan Academy of Agricultural Sciences, Changsha 410125, ChinaLongping Branch Graduate School, Hunan University, Changsha 410125, ChinaInstitute of Plant Protection, Hunan Academy of Agricultural Sciences, Changsha 410125, ChinaLongping Branch Graduate School, Hunan University, Changsha 410125, ChinaDepartment of Entomology, University of Kentucky, Lexington, KY 40546, USATransmission of plant pathogenic viruses mostly relies on insect vectors. Plant virus could enhance its transmission by modulating the vector. Previously, we showed that feeding on virus infected plants can promote the reproduction of the sweet potato whitefly, <i>Bemisia tabaci</i> MED (Q biotype). In this study, using a whitefly-Tomato chlorosis virus (ToCV)-tomato system, we investigated how ToCV modulates <i>B. tabaci</i> MED reproduction to facilitate its spread. Here, we hypothesized that ToCV-infected tomato plants would increase <i>B. tabaci</i> MED fecundity via elevated <i>vitellogenin (Vg)</i> gene expression. As a result, fecundity and the relative expression of <i>B. tabaci</i> MED <i>Vg</i> was measured on ToCV-infected and uninfected tomato plants on days 4, 8, 12, 16, 20 and 24. The role of <i>Vg</i> on <i>B. tabaci</i> MED reproduction was examined in the presence and absence of ToCV using dietary RNAi. ToCV infection significantly increased <i>B. tabaci</i> MED fecundity on days 12, 16 and 20, and elevated <i>Vg</i> expression on days 8, 12 and 16. Both ovarian development and fecundity of <i>B. tabaci</i> MED were suppressed when <i>Vg</i> was silenced with or without ToCV infection. These combined results suggest that ToCV infection increases <i>B. tabaci</i> MED fecundity via elevated <i>Vg</i> expression.https://www.mdpi.com/2075-4450/12/2/101<i>Bemisia tabaci</i>fecundityovarian developmentgene expressionRNA interference<i>vitellogenin</i> |
| spellingShingle | Liping Huang Xiaobin Shi Jizhe Shi Zhuo Zhang Yong Fang Zhanhong Zhang Qiuyi Pan Limin Zheng Yang Gao Deyong Zhang Xinqiu Tan Yong Liu Xuguo Zhou Tomato Chlorosis Virus Infection Facilitates <i>Bemisia tabaci</i> MED Reproduction by Elevating <i>Vitellogenin</i> Expression Insects <i>Bemisia tabaci</i> fecundity ovarian development gene expression RNA interference <i>vitellogenin</i> |
| title | Tomato Chlorosis Virus Infection Facilitates <i>Bemisia tabaci</i> MED Reproduction by Elevating <i>Vitellogenin</i> Expression |
| title_full | Tomato Chlorosis Virus Infection Facilitates <i>Bemisia tabaci</i> MED Reproduction by Elevating <i>Vitellogenin</i> Expression |
| title_fullStr | Tomato Chlorosis Virus Infection Facilitates <i>Bemisia tabaci</i> MED Reproduction by Elevating <i>Vitellogenin</i> Expression |
| title_full_unstemmed | Tomato Chlorosis Virus Infection Facilitates <i>Bemisia tabaci</i> MED Reproduction by Elevating <i>Vitellogenin</i> Expression |
| title_short | Tomato Chlorosis Virus Infection Facilitates <i>Bemisia tabaci</i> MED Reproduction by Elevating <i>Vitellogenin</i> Expression |
| title_sort | tomato chlorosis virus infection facilitates i bemisia tabaci i med reproduction by elevating i vitellogenin i expression |
| topic | <i>Bemisia tabaci</i> fecundity ovarian development gene expression RNA interference <i>vitellogenin</i> |
| url | https://www.mdpi.com/2075-4450/12/2/101 |
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