Gut microbiota in the association between obesity and kidney function decline: a metagenomics-based study in a rat model
AbstractObjectives Obesity can induce dysbiosis in the gut microbiota and is considered a separate risk factor for kidney function decline. Nonetheless, the precise function of intestinal microorganisms in facilitating the connection between obesity and kidney function decline remains uncertain. Hen...
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| Format: | Article |
| Language: | English |
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Taylor & Francis Group
2024-12-01
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| Series: | Renal Failure |
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| Online Access: | https://www.tandfonline.com/doi/10.1080/0886022X.2024.2328320 |
| _version_ | 1797265278399152128 |
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| author | Akehu Alemasi Lijiang Gu Yilun Zhou |
| author_facet | Akehu Alemasi Lijiang Gu Yilun Zhou |
| author_sort | Akehu Alemasi |
| collection | DOAJ |
| description | AbstractObjectives Obesity can induce dysbiosis in the gut microbiota and is considered a separate risk factor for kidney function decline. Nonetheless, the precise function of intestinal microorganisms in facilitating the connection between obesity and kidney function decline remains uncertain. Hence, the objective of this study was to investigate the alterations in the gut microbiota composition that take place during obesity and their correlations with renal function utilizing a rat model.Methods For 20 weeks, 25 Sprague–Dawley rats were fed either a high-fat diet (HFD) or a normal-fat normal diet (ND). Physiological indices, peripheral plasma, kidney tissue, and colon contents were collected for comparison between groups. Metagenomic analysis of intestinal flora was performed.Results The HFD group demonstrated significantly increased levels of creatinine and urea nitrogen in the peripheral blood. Additionally, the HFD rats exhibited a significantly larger glomerular diameter compared to the ND group, accompanied by the presence of glomerulosclerosis, tubular vacuolar transformation, and other pathological changes in certain glomeruli. Metagenomics analysis revealed a notable rise in the prevalence of the Firmicutes phylum within the HFD group, primarily comprising the Rumenococcus genus. Functional analysis indicated that the gut microbiota in the HFD group primarily correlated with infectious diseases, signal transduction, and signaling molecules and interactions.Conclusions This study provides evidence that the consumption of a HFD induces modifications in the composition and functionality of the gut microbiome in rats, which may serve as a potential mechanism underlying the relationship between obesity and the progression of kidney function decline. |
| first_indexed | 2024-04-25T00:42:15Z |
| format | Article |
| id | doaj.art-c00a89f4951143a08c236a5823712182 |
| institution | Directory Open Access Journal |
| issn | 0886-022X 1525-6049 |
| language | English |
| last_indexed | 2024-04-25T00:42:15Z |
| publishDate | 2024-12-01 |
| publisher | Taylor & Francis Group |
| record_format | Article |
| series | Renal Failure |
| spelling | doaj.art-c00a89f4951143a08c236a58237121822024-03-12T08:15:43ZengTaylor & Francis GroupRenal Failure0886-022X1525-60492024-12-0146110.1080/0886022X.2024.2328320Gut microbiota in the association between obesity and kidney function decline: a metagenomics-based study in a rat modelAkehu Alemasi0Lijiang Gu1Yilun Zhou2Department of Nephrology, Beijing Tiantan Hospital, Capital Medical University, Beijing, ChinaDepartment of Urology, the First Affiliated Hospital of Xi’an Jiaotong University, Xi’an, Shaanxi, ChinaDepartment of Nephrology, Beijing Tiantan Hospital, Capital Medical University, Beijing, ChinaAbstractObjectives Obesity can induce dysbiosis in the gut microbiota and is considered a separate risk factor for kidney function decline. Nonetheless, the precise function of intestinal microorganisms in facilitating the connection between obesity and kidney function decline remains uncertain. Hence, the objective of this study was to investigate the alterations in the gut microbiota composition that take place during obesity and their correlations with renal function utilizing a rat model.Methods For 20 weeks, 25 Sprague–Dawley rats were fed either a high-fat diet (HFD) or a normal-fat normal diet (ND). Physiological indices, peripheral plasma, kidney tissue, and colon contents were collected for comparison between groups. Metagenomic analysis of intestinal flora was performed.Results The HFD group demonstrated significantly increased levels of creatinine and urea nitrogen in the peripheral blood. Additionally, the HFD rats exhibited a significantly larger glomerular diameter compared to the ND group, accompanied by the presence of glomerulosclerosis, tubular vacuolar transformation, and other pathological changes in certain glomeruli. Metagenomics analysis revealed a notable rise in the prevalence of the Firmicutes phylum within the HFD group, primarily comprising the Rumenococcus genus. Functional analysis indicated that the gut microbiota in the HFD group primarily correlated with infectious diseases, signal transduction, and signaling molecules and interactions.Conclusions This study provides evidence that the consumption of a HFD induces modifications in the composition and functionality of the gut microbiome in rats, which may serve as a potential mechanism underlying the relationship between obesity and the progression of kidney function decline.https://www.tandfonline.com/doi/10.1080/0886022X.2024.2328320Obesity-associated kidney injuryintestinal florametagenomicsinfectious diseasecytokines |
| spellingShingle | Akehu Alemasi Lijiang Gu Yilun Zhou Gut microbiota in the association between obesity and kidney function decline: a metagenomics-based study in a rat model Renal Failure Obesity-associated kidney injury intestinal flora metagenomics infectious disease cytokines |
| title | Gut microbiota in the association between obesity and kidney function decline: a metagenomics-based study in a rat model |
| title_full | Gut microbiota in the association between obesity and kidney function decline: a metagenomics-based study in a rat model |
| title_fullStr | Gut microbiota in the association between obesity and kidney function decline: a metagenomics-based study in a rat model |
| title_full_unstemmed | Gut microbiota in the association between obesity and kidney function decline: a metagenomics-based study in a rat model |
| title_short | Gut microbiota in the association between obesity and kidney function decline: a metagenomics-based study in a rat model |
| title_sort | gut microbiota in the association between obesity and kidney function decline a metagenomics based study in a rat model |
| topic | Obesity-associated kidney injury intestinal flora metagenomics infectious disease cytokines |
| url | https://www.tandfonline.com/doi/10.1080/0886022X.2024.2328320 |
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