Over-expression of miR-210 inhibits H2O2-induced adipogenic differentiation of rat BM-MSCs
Objective To investigate the effect of miR-210 on hydrogen peroxide (H2O2)-induced adipogenic differentiation of rat bone marrow mesenchymal stem cells (BM-MSCs). Methods The SD rat BM-MSCs were isolated, cultured and identified. BM-MSCs were induced by 200 μmol/L H2O2 for 1 h to construct an adip...
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| Format: | Article |
| Language: | zho |
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Institute of Basic Medical Sciences and Peking Union Medical College Hospital, Chinese Academy of Medical Sciences / Peking Union Medical College.
2023-02-01
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| Series: | Jichu yixue yu linchuang |
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| Online Access: | http://journal11.magtechjournal.com/Jwk_jcyxylc/fileup/1001-6325/PDF/1001-6325-2023-43-2-271.pdf |
| _version_ | 1827391067783168000 |
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| author | FENG Baohong, YU Yean, YAN Li, WU Jun, ZHANG Yanxia, ZHU Geli |
| author_facet | FENG Baohong, YU Yean, YAN Li, WU Jun, ZHANG Yanxia, ZHU Geli |
| author_sort | FENG Baohong, YU Yean, YAN Li, WU Jun, ZHANG Yanxia, ZHU Geli |
| collection | DOAJ |
| description | Objective To investigate the effect of miR-210 on hydrogen peroxide (H2O2)-induced adipogenic differentiation of rat bone marrow mesenchymal stem cells (BM-MSCs). Methods The SD rat BM-MSCs were isolated, cultured and identified. BM-MSCs were induced by 200 μmol/L H2O2 for 1 h to construct an adipogenic differentiation model. Adipogenic differentiation models were transfected with miR-210 mimic or inhibitor, respectively. RT-qPCR to detect the expression of miR-210; oil red O staining to detect the fat particles in the cytoplasm of cells; Western blot to detect the expression of peroxisome proliferator-activated receptor γ (PPARγ), CCAAT enhancer binding protein (C/EBPa) and Wnt/β-catenin pathway related proteins of cells. Results SD rat BM-MSCs were successfully isolated. In adipogenic differentiation model of BM-MSCs, the levels of miR-210, β-catenin, c-Myc, and cyclin D1 protein decreased, while the number of fat granules, PPARγ and C/EBPα protein levels increased; In BM-MSCs transfected with miR-210 mimic, the levels of miR-210, β-catenin, c-Myc, and cyclin D1 protein increased, while the fat granules, PPARγ, and C/EBPα protein level all decreased; The change trend of corresponding indexes in BM-MSCs transfected with miR-210 inhibitor was opposite to that in BM-MSCs transfected with miR-210 mimic. Conclusions Over-expression of miR-210 could inhibit the adipogenic differentiation of BM-MSCs induced by H2O2. This mechanism may be related to the activation of Wnt/β-catenin pathway. |
| first_indexed | 2024-03-08T17:03:21Z |
| format | Article |
| id | doaj.art-c00c2335d79a421aa4aeb2c16bc3c4a7 |
| institution | Directory Open Access Journal |
| issn | 1001-6325 |
| language | zho |
| last_indexed | 2024-03-08T17:03:21Z |
| publishDate | 2023-02-01 |
| publisher | Institute of Basic Medical Sciences and Peking Union Medical College Hospital, Chinese Academy of Medical Sciences / Peking Union Medical College. |
| record_format | Article |
| series | Jichu yixue yu linchuang |
| spelling | doaj.art-c00c2335d79a421aa4aeb2c16bc3c4a72024-01-04T07:27:29ZzhoInstitute of Basic Medical Sciences and Peking Union Medical College Hospital, Chinese Academy of Medical Sciences / Peking Union Medical College.Jichu yixue yu linchuang1001-63252023-02-0143227127610.16352/j.issn.1001-6325.2023.02.271Over-expression of miR-210 inhibits H2O2-induced adipogenic differentiation of rat BM-MSCsFENG Baohong, YU Yean, YAN Li, WU Jun, ZHANG Yanxia, ZHU Geli0Department of Nephrology, the Third Affiliated Hospital of Wuhan University, Wuhan 430014, ChinaObjective To investigate the effect of miR-210 on hydrogen peroxide (H2O2)-induced adipogenic differentiation of rat bone marrow mesenchymal stem cells (BM-MSCs). Methods The SD rat BM-MSCs were isolated, cultured and identified. BM-MSCs were induced by 200 μmol/L H2O2 for 1 h to construct an adipogenic differentiation model. Adipogenic differentiation models were transfected with miR-210 mimic or inhibitor, respectively. RT-qPCR to detect the expression of miR-210; oil red O staining to detect the fat particles in the cytoplasm of cells; Western blot to detect the expression of peroxisome proliferator-activated receptor γ (PPARγ), CCAAT enhancer binding protein (C/EBPa) and Wnt/β-catenin pathway related proteins of cells. Results SD rat BM-MSCs were successfully isolated. In adipogenic differentiation model of BM-MSCs, the levels of miR-210, β-catenin, c-Myc, and cyclin D1 protein decreased, while the number of fat granules, PPARγ and C/EBPα protein levels increased; In BM-MSCs transfected with miR-210 mimic, the levels of miR-210, β-catenin, c-Myc, and cyclin D1 protein increased, while the fat granules, PPARγ, and C/EBPα protein level all decreased; The change trend of corresponding indexes in BM-MSCs transfected with miR-210 inhibitor was opposite to that in BM-MSCs transfected with miR-210 mimic. Conclusions Over-expression of miR-210 could inhibit the adipogenic differentiation of BM-MSCs induced by H2O2. This mechanism may be related to the activation of Wnt/β-catenin pathway.http://journal11.magtechjournal.com/Jwk_jcyxylc/fileup/1001-6325/PDF/1001-6325-2023-43-2-271.pdfmir-210|hydrogen peroxide|bone marrow mesenchymal stem cells|adipogenic differentiation |
| spellingShingle | FENG Baohong, YU Yean, YAN Li, WU Jun, ZHANG Yanxia, ZHU Geli Over-expression of miR-210 inhibits H2O2-induced adipogenic differentiation of rat BM-MSCs Jichu yixue yu linchuang mir-210|hydrogen peroxide|bone marrow mesenchymal stem cells|adipogenic differentiation |
| title | Over-expression of miR-210 inhibits H2O2-induced adipogenic differentiation of rat BM-MSCs |
| title_full | Over-expression of miR-210 inhibits H2O2-induced adipogenic differentiation of rat BM-MSCs |
| title_fullStr | Over-expression of miR-210 inhibits H2O2-induced adipogenic differentiation of rat BM-MSCs |
| title_full_unstemmed | Over-expression of miR-210 inhibits H2O2-induced adipogenic differentiation of rat BM-MSCs |
| title_short | Over-expression of miR-210 inhibits H2O2-induced adipogenic differentiation of rat BM-MSCs |
| title_sort | over expression of mir 210 inhibits h2o2 induced adipogenic differentiation of rat bm mscs |
| topic | mir-210|hydrogen peroxide|bone marrow mesenchymal stem cells|adipogenic differentiation |
| url | http://journal11.magtechjournal.com/Jwk_jcyxylc/fileup/1001-6325/PDF/1001-6325-2023-43-2-271.pdf |
| work_keys_str_mv | AT fengbaohongyuyeanyanliwujunzhangyanxiazhugeli overexpressionofmir210inhibitsh2o2inducedadipogenicdifferentiationofratbmmscs |