Characterization of smoke and aerosol deliveries from combustible cigarettes, heated tobacco products and electronic nicotine delivery systems in the Vitrocell® Mammalian 6/48 exposure module
The rapid development associated with Next Generation Tobacco Products (NGTP) has necessitated the development of high throughput methodologies to test their genotoxic potential in vitro when compared to conventional cigarette smoke (CS). An assessment of two Vitrocell® Mammalian 6/48 exposure modul...
| Main Authors: | , , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Elsevier
2022-01-01
|
| Series: | Toxicology Reports |
| Subjects: | |
| Online Access: | http://www.sciencedirect.com/science/article/pii/S2214750022002141 |
| _version_ | 1811178660605984768 |
|---|---|
| author | Brian M. Keyser Robert Leverette Michael Hollings Adam Seymour Randy A. Weidman Carlton J. Bequette Kristen Jordan |
| author_facet | Brian M. Keyser Robert Leverette Michael Hollings Adam Seymour Randy A. Weidman Carlton J. Bequette Kristen Jordan |
| author_sort | Brian M. Keyser |
| collection | DOAJ |
| description | The rapid development associated with Next Generation Tobacco Products (NGTP) has necessitated the development of high throughput methodologies to test their genotoxic potential in vitro when compared to conventional cigarette smoke (CS). An assessment of two Vitrocell® Mammalian 6/48 exposure modules in three independent experiments was made by comparing results from multiple dosimetric techniques applied to aerosol generated from 3R4F Kentucky Reference cigarettes, commercially available electronically heated tobacco product (eHTP) and Electronic Nicotine Delivery System (ENDS) using the Vitrocell® VC10®. Real-time aerosol particle concentration was assessed by means of light scattering photometers and expressed as area under the curve (∑AUC). Nicotine concentrations were determined analytically by LC/MS. Humectant amount and distribution was assessed for eHTP and ENDS by the quantification of free glycerol in a phosphate buffered saline (PBS) trap, whereas total particulate matter (TPM) was assessed in the 3R4F cigarettes by the fluorescence of the particulate at 485 nm in anhydrous dimethyl sulfoxide (DMSO) trap within the exposure. Dose was adjusted by means of the addition of ambient air to dilute the whole smoke/aerosol in L/min and sampled into the system at a rate of 5 mL/min. Dilution of CS ranged from 8.0 to 0.5 L/min and for the eHTP and ENDS ranged from 4 to 0 L/min (undiluted). Dosimetric analysis of the system showed good concordance within replicates (p-values ranged from p = 0.3762 to p = 0.8926) and showed that the Vitrocell® Mammalian 6/48 is a viable means for genotoxic assessment of aerosol generated from both conventional cigarettes and NGTP. Results demonstrate the need to tailor dosimetry approaches to different aerosols due to variations in the physio-chemical composition, with a multi-dosimetry approach recommended. |
| first_indexed | 2024-04-11T06:22:08Z |
| format | Article |
| id | doaj.art-c010c6872662426d8f32d1c126f9a108 |
| institution | Directory Open Access Journal |
| issn | 2214-7500 |
| language | English |
| last_indexed | 2024-04-11T06:22:08Z |
| publishDate | 2022-01-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Toxicology Reports |
| spelling | doaj.art-c010c6872662426d8f32d1c126f9a1082022-12-22T04:40:32ZengElsevierToxicology Reports2214-75002022-01-01919851992Characterization of smoke and aerosol deliveries from combustible cigarettes, heated tobacco products and electronic nicotine delivery systems in the Vitrocell® Mammalian 6/48 exposure moduleBrian M. Keyser0Robert Leverette1Michael Hollings2Adam Seymour3Randy A. Weidman4Carlton J. Bequette5Kristen Jordan6RAI Services Company, Scientific & Regulatory Affairs, 401 North Main Street, Winston-Salem, NC 27101, USA; Corresponding authors.RAI Services Company, Scientific & Regulatory Affairs, 401 North Main Street, Winston-Salem, NC 27101, USA; Corresponding authors.Labcorp Early Development Laboratories Ltd., Harrogate, North Yorkshire, UKLabcorp Early Development Laboratories Ltd., Harrogate, North Yorkshire, UKRJ Reynolds Tobacco Company, 950 Reynolds Blvd., Winston-Salem, NC 27106, USARJ Reynolds Tobacco Company, 950 Reynolds Blvd., Winston-Salem, NC 27106, USARAI Services Company, Scientific & Regulatory Affairs, 401 North Main Street, Winston-Salem, NC 27101, USAThe rapid development associated with Next Generation Tobacco Products (NGTP) has necessitated the development of high throughput methodologies to test their genotoxic potential in vitro when compared to conventional cigarette smoke (CS). An assessment of two Vitrocell® Mammalian 6/48 exposure modules in three independent experiments was made by comparing results from multiple dosimetric techniques applied to aerosol generated from 3R4F Kentucky Reference cigarettes, commercially available electronically heated tobacco product (eHTP) and Electronic Nicotine Delivery System (ENDS) using the Vitrocell® VC10®. Real-time aerosol particle concentration was assessed by means of light scattering photometers and expressed as area under the curve (∑AUC). Nicotine concentrations were determined analytically by LC/MS. Humectant amount and distribution was assessed for eHTP and ENDS by the quantification of free glycerol in a phosphate buffered saline (PBS) trap, whereas total particulate matter (TPM) was assessed in the 3R4F cigarettes by the fluorescence of the particulate at 485 nm in anhydrous dimethyl sulfoxide (DMSO) trap within the exposure. Dose was adjusted by means of the addition of ambient air to dilute the whole smoke/aerosol in L/min and sampled into the system at a rate of 5 mL/min. Dilution of CS ranged from 8.0 to 0.5 L/min and for the eHTP and ENDS ranged from 4 to 0 L/min (undiluted). Dosimetric analysis of the system showed good concordance within replicates (p-values ranged from p = 0.3762 to p = 0.8926) and showed that the Vitrocell® Mammalian 6/48 is a viable means for genotoxic assessment of aerosol generated from both conventional cigarettes and NGTP. Results demonstrate the need to tailor dosimetry approaches to different aerosols due to variations in the physio-chemical composition, with a multi-dosimetry approach recommended.http://www.sciencedirect.com/science/article/pii/S2214750022002141Whole smokeWhole aerosolDosimetryNicotine |
| spellingShingle | Brian M. Keyser Robert Leverette Michael Hollings Adam Seymour Randy A. Weidman Carlton J. Bequette Kristen Jordan Characterization of smoke and aerosol deliveries from combustible cigarettes, heated tobacco products and electronic nicotine delivery systems in the Vitrocell® Mammalian 6/48 exposure module Toxicology Reports Whole smoke Whole aerosol Dosimetry Nicotine |
| title | Characterization of smoke and aerosol deliveries from combustible cigarettes, heated tobacco products and electronic nicotine delivery systems in the Vitrocell® Mammalian 6/48 exposure module |
| title_full | Characterization of smoke and aerosol deliveries from combustible cigarettes, heated tobacco products and electronic nicotine delivery systems in the Vitrocell® Mammalian 6/48 exposure module |
| title_fullStr | Characterization of smoke and aerosol deliveries from combustible cigarettes, heated tobacco products and electronic nicotine delivery systems in the Vitrocell® Mammalian 6/48 exposure module |
| title_full_unstemmed | Characterization of smoke and aerosol deliveries from combustible cigarettes, heated tobacco products and electronic nicotine delivery systems in the Vitrocell® Mammalian 6/48 exposure module |
| title_short | Characterization of smoke and aerosol deliveries from combustible cigarettes, heated tobacco products and electronic nicotine delivery systems in the Vitrocell® Mammalian 6/48 exposure module |
| title_sort | characterization of smoke and aerosol deliveries from combustible cigarettes heated tobacco products and electronic nicotine delivery systems in the vitrocell r mammalian 6 48 exposure module |
| topic | Whole smoke Whole aerosol Dosimetry Nicotine |
| url | http://www.sciencedirect.com/science/article/pii/S2214750022002141 |
| work_keys_str_mv | AT brianmkeyser characterizationofsmokeandaerosoldeliveriesfromcombustiblecigarettesheatedtobaccoproductsandelectronicnicotinedeliverysystemsinthevitrocellmammalian648exposuremodule AT robertleverette characterizationofsmokeandaerosoldeliveriesfromcombustiblecigarettesheatedtobaccoproductsandelectronicnicotinedeliverysystemsinthevitrocellmammalian648exposuremodule AT michaelhollings characterizationofsmokeandaerosoldeliveriesfromcombustiblecigarettesheatedtobaccoproductsandelectronicnicotinedeliverysystemsinthevitrocellmammalian648exposuremodule AT adamseymour characterizationofsmokeandaerosoldeliveriesfromcombustiblecigarettesheatedtobaccoproductsandelectronicnicotinedeliverysystemsinthevitrocellmammalian648exposuremodule AT randyaweidman characterizationofsmokeandaerosoldeliveriesfromcombustiblecigarettesheatedtobaccoproductsandelectronicnicotinedeliverysystemsinthevitrocellmammalian648exposuremodule AT carltonjbequette characterizationofsmokeandaerosoldeliveriesfromcombustiblecigarettesheatedtobaccoproductsandelectronicnicotinedeliverysystemsinthevitrocellmammalian648exposuremodule AT kristenjordan characterizationofsmokeandaerosoldeliveriesfromcombustiblecigarettesheatedtobaccoproductsandelectronicnicotinedeliverysystemsinthevitrocellmammalian648exposuremodule |