Cordycepin inhibits bronchial epithelial-mesenchymal transition in asthmatic rats
Objective To investigate the mechanism of cordycepin (Cor) in the inhibition of epithelial-mesenchymal transition in asthmatic rats. Methods Rats were randomly divided into 4 groups (n=10): control group, ovalbumin (OVA) group, OVA+10 mg/kg Cor group, OVA+50 mg/kg Cor group. After 7 days of treatmen...
| Main Author: | |
|---|---|
| Format: | Article |
| Language: | zho |
| Published: |
Institute of Basic Medical Sciences and Peking Union Medical College Hospital, Chinese Academy of Medical Sciences / Peking Union Medical College.
2022-04-01
|
| Series: | Jichu yixue yu linchuang |
| Subjects: | |
| Online Access: | http://journal11.magtechjournal.com/Jwk_jcyxylc/fileup/1001-6325/PDF/1001-6325-2022-42-4-607.pdf |
| _version_ | 1797361454601469952 |
|---|---|
| author | TANG Li-ping, YAN Jin, FAN Fang, WANG Hao, HUANG Na |
| author_facet | TANG Li-ping, YAN Jin, FAN Fang, WANG Hao, HUANG Na |
| author_sort | TANG Li-ping, YAN Jin, FAN Fang, WANG Hao, HUANG Na |
| collection | DOAJ |
| description | Objective To investigate the mechanism of cordycepin (Cor) in the inhibition of epithelial-mesenchymal transition in asthmatic rats. Methods Rats were randomly divided into 4 groups (n=10): control group, ovalbumin (OVA) group, OVA+10 mg/kg Cor group, OVA+50 mg/kg Cor group. After 7 days of treatment, the cell counting in bronchoalveolar lavage fluid (BALF) was measured; hematoxylin-eosin (HE) staining was used to evaluate airway inflammation and airway remodeling. Masson's trichrome staining was used to detect epithelial collagen deposition. Human bronchial epithelioid cells 16HBE were divided into 3 groups: control group, TGF-β1 group and TGF-β1+Cor group. Immuno-fluorescence staining was used to detect the expression of c-Jun in the cells. Cell proliferation and migration were examined by cell counting kit-8 (CCK-8) and Transwell. The protein expression of E-cadherin, N-cadherin, α-SMA, vimentin, Smad3, p-Smad3, ERK1/2 and p-ERK1/2 was detected by immuno-histochemical staining or Western blot. Results With comparison with specific control groups, the following results were recorded. The collagen fiber area and cell count of OVA group increased significantly(P＜0.05). The collagen fiber area and cell count of OVA+10 mg/kg Cor group and OVA+50 mg/kg Cor group both decreased (P＜0.05). E-cadherin staining degree and protein expression of OVA group decreased, while the staining degree of α-SMA increased and the protein expression of N-cadherin, α-SMA and vimentin increased (P＜0.05); E-cadherin staining degree and protein expression of OVA+10 mg/kg Cor group and OVA+50 mg/kg Cor group increased, while the staining degree of α-SMA decreased and the protein expression of N-cadherin, α-SMA and vimentin decreased (P＜0.05). Compared with control group, the cell viability and the number of migrating cells in the TGF-β1 group increased (P＜0.05); compared with the TGF-β1 group, the cell viability and the number of migrating cells in the TGF-β1+Cor group were both decreased (P＜0.05). The expression level of E-cadherin protein in TGF-β1 group decreased, while the protein expression of N-cadherin, α-SMA, vimentin, p-Smad3 and p-ERK1/2 increased (P＜0.05). The E-cadherin protein expression level of TGF-β1+Cor group increased, while the N-cadherin, α-SMA, vimentin, p-Smad3 and p-ERK1/2 protein expression levels decreased (P＜0.05). The relative fluorescence intensity of c-Jun in TGF-β1 group increased (P＜0.05); The relative fluorescence intensity of c-Jun in TGF-β1+Cor group decreased (P＜0.05). Conclusions Cordycepin inhibits EMT in asthma by inhibiting Smad3 and ERK1/2 signaling pathway and c-Jun. |
| first_indexed | 2024-03-08T15:53:56Z |
| format | Article |
| id | doaj.art-c013580fad314a0d8d807c397028a409 |
| institution | Directory Open Access Journal |
| issn | 1001-6325 |
| language | zho |
| last_indexed | 2024-03-08T15:53:56Z |
| publishDate | 2022-04-01 |
| publisher | Institute of Basic Medical Sciences and Peking Union Medical College Hospital, Chinese Academy of Medical Sciences / Peking Union Medical College. |
| record_format | Article |
| series | Jichu yixue yu linchuang |
| spelling | doaj.art-c013580fad314a0d8d807c397028a4092024-01-09T02:51:38ZzhoInstitute of Basic Medical Sciences and Peking Union Medical College Hospital, Chinese Academy of Medical Sciences / Peking Union Medical College.Jichu yixue yu linchuang1001-63252022-04-0142460761510.16352/j.issn.1001-6325.2022.04.022Cordycepin inhibits bronchial epithelial-mesenchymal transition in asthmatic ratsTANG Li-ping, YAN Jin, FAN Fang, WANG Hao, HUANG Na01 Department of Pediatrics, Xijing Hospital, Air Force Military Medical University, Xi'an 710032; ;2. College of Medical Technology, Xi'an Medical University, Xi'an 710021, ChinaObjective To investigate the mechanism of cordycepin (Cor) in the inhibition of epithelial-mesenchymal transition in asthmatic rats. Methods Rats were randomly divided into 4 groups (n=10): control group, ovalbumin (OVA) group, OVA+10 mg/kg Cor group, OVA+50 mg/kg Cor group. After 7 days of treatment, the cell counting in bronchoalveolar lavage fluid (BALF) was measured; hematoxylin-eosin (HE) staining was used to evaluate airway inflammation and airway remodeling. Masson's trichrome staining was used to detect epithelial collagen deposition. Human bronchial epithelioid cells 16HBE were divided into 3 groups: control group, TGF-β1 group and TGF-β1+Cor group. Immuno-fluorescence staining was used to detect the expression of c-Jun in the cells. Cell proliferation and migration were examined by cell counting kit-8 (CCK-8) and Transwell. The protein expression of E-cadherin, N-cadherin, α-SMA, vimentin, Smad3, p-Smad3, ERK1/2 and p-ERK1/2 was detected by immuno-histochemical staining or Western blot. Results With comparison with specific control groups, the following results were recorded. The collagen fiber area and cell count of OVA group increased significantly(P＜0.05). The collagen fiber area and cell count of OVA+10 mg/kg Cor group and OVA+50 mg/kg Cor group both decreased (P＜0.05). E-cadherin staining degree and protein expression of OVA group decreased, while the staining degree of α-SMA increased and the protein expression of N-cadherin, α-SMA and vimentin increased (P＜0.05); E-cadherin staining degree and protein expression of OVA+10 mg/kg Cor group and OVA+50 mg/kg Cor group increased, while the staining degree of α-SMA decreased and the protein expression of N-cadherin, α-SMA and vimentin decreased (P＜0.05). Compared with control group, the cell viability and the number of migrating cells in the TGF-β1 group increased (P＜0.05); compared with the TGF-β1 group, the cell viability and the number of migrating cells in the TGF-β1+Cor group were both decreased (P＜0.05). The expression level of E-cadherin protein in TGF-β1 group decreased, while the protein expression of N-cadherin, α-SMA, vimentin, p-Smad3 and p-ERK1/2 increased (P＜0.05). The E-cadherin protein expression level of TGF-β1+Cor group increased, while the N-cadherin, α-SMA, vimentin, p-Smad3 and p-ERK1/2 protein expression levels decreased (P＜0.05). The relative fluorescence intensity of c-Jun in TGF-β1 group increased (P＜0.05); The relative fluorescence intensity of c-Jun in TGF-β1+Cor group decreased (P＜0.05). Conclusions Cordycepin inhibits EMT in asthma by inhibiting Smad3 and ERK1/2 signaling pathway and c-Jun.http://journal11.magtechjournal.com/Jwk_jcyxylc/fileup/1001-6325/PDF/1001-6325-2022-42-4-607.pdfcordycepin|asthma|epithelial-mesenchymal transition|bronchus|human bronchial epithelioid cells |
| spellingShingle | TANG Li-ping, YAN Jin, FAN Fang, WANG Hao, HUANG Na Cordycepin inhibits bronchial epithelial-mesenchymal transition in asthmatic rats Jichu yixue yu linchuang cordycepin|asthma|epithelial-mesenchymal transition|bronchus|human bronchial epithelioid cells |
| title | Cordycepin inhibits bronchial epithelial-mesenchymal transition in asthmatic rats |
| title_full | Cordycepin inhibits bronchial epithelial-mesenchymal transition in asthmatic rats |
| title_fullStr | Cordycepin inhibits bronchial epithelial-mesenchymal transition in asthmatic rats |
| title_full_unstemmed | Cordycepin inhibits bronchial epithelial-mesenchymal transition in asthmatic rats |
| title_short | Cordycepin inhibits bronchial epithelial-mesenchymal transition in asthmatic rats |
| title_sort | cordycepin inhibits bronchial epithelial mesenchymal transition in asthmatic rats |
| topic | cordycepin|asthma|epithelial-mesenchymal transition|bronchus|human bronchial epithelioid cells |
| url | http://journal11.magtechjournal.com/Jwk_jcyxylc/fileup/1001-6325/PDF/1001-6325-2022-42-4-607.pdf |
| work_keys_str_mv | AT tanglipingyanjinfanfangwanghaohuangna cordycepininhibitsbronchialepithelialmesenchymaltransitioninasthmaticrats |