Control of microorganisms in vitro by endodontic irrigants
The aim of this study was to determine the minimum inhibitory concentration (MIC) and antimicrobial effectiveness by the direct exposure test of 4 endodontic irrigants [1% sodium hypochlorite (NaOCl), 2% chlorhexidine (CHX), 1% calcium hydroxide (Ca(OH)2; prepared with 1 g of Ca(OH)2 and 100 mL of s...
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| Format: | Article |
| Language: | English |
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Fundação Odontológica de Ribeirão Preto
2003-01-01
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| Series: | Brazilian Dental Journal |
| Subjects: | |
| Online Access: | http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0103-64402003000300009 |
| _version_ | 1818469109351841792 |
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| author | Estrela Cyntia R.A. Estrela Carlos Reis Cleômenes Bammann Lili Luschke Pécora Jesus Djalma |
| author_facet | Estrela Cyntia R.A. Estrela Carlos Reis Cleômenes Bammann Lili Luschke Pécora Jesus Djalma |
| author_sort | Estrela Cyntia R.A. |
| collection | DOAJ |
| description | The aim of this study was to determine the minimum inhibitory concentration (MIC) and antimicrobial effectiveness by the direct exposure test of 4 endodontic irrigants [1% sodium hypochlorite (NaOCl), 2% chlorhexidine (CHX), 1% calcium hydroxide (Ca(OH)2; prepared with 1 g of Ca(OH)2 and 100 mL of sterile distilled water), a solution of Ca(OH)2 + detergent (HCT20)] for S. aureus, E. faecalis, P. aeruginosa, B. subtilis, C. albicans and a mixed culture. Microbial growth was analyzed by two methods: turbidity of the culture medium that was confirmed by Gram stain and subculture in a specific nutrient broth. In the dilution test, NaOCl solution showed MIC equal to 0.1% for S. aureus, E. faecalis, P. aeruginosa and C. albicans and equal to 1% for B. subtilis and the mixed culture. CHX (2%) presented MIC equal to 0.000002% for S. aureus, 0.02% for E. faecalis, B. subtilis, C. albicans and the mixed culture and 0.002% for P. aeruginosa. Ca(OH)2 solution (1%) showed MIC greater than 1% for all the microorganisms except P. aeruginosa for which it was equal to 1%. Calcium hydroxide solution + detergent showed MIC equal to 4.5 mL for S. aureus, P. aeruginosa, B. subtilis, C. albicans and the mixed culture and greater than 4.5 mL for E. faecalis. In the direct exposure test, NaOCl had better antimicrobial effectiveness for all microorganisms at all times. CHX (2%) was effective for S. aureus, E. faecalis and C. albicans at all times, and ineffective for P. aeruginosa, B. subtilis and the mixed culture. The other solutions showed the worst results. |
| first_indexed | 2024-04-13T21:19:49Z |
| format | Article |
| id | doaj.art-c01c099b6e184c82a90e0ca26503c90a |
| institution | Directory Open Access Journal |
| issn | 0103-6440 |
| language | English |
| last_indexed | 2024-04-13T21:19:49Z |
| publishDate | 2003-01-01 |
| publisher | Fundação Odontológica de Ribeirão Preto |
| record_format | Article |
| series | Brazilian Dental Journal |
| spelling | doaj.art-c01c099b6e184c82a90e0ca26503c90a2022-12-22T02:29:33ZengFundação Odontológica de Ribeirão PretoBrazilian Dental Journal0103-64402003-01-01143187192Control of microorganisms in vitro by endodontic irrigantsEstrela Cyntia R.A.Estrela CarlosReis CleômenesBammann Lili LuschkePécora Jesus DjalmaThe aim of this study was to determine the minimum inhibitory concentration (MIC) and antimicrobial effectiveness by the direct exposure test of 4 endodontic irrigants [1% sodium hypochlorite (NaOCl), 2% chlorhexidine (CHX), 1% calcium hydroxide (Ca(OH)2; prepared with 1 g of Ca(OH)2 and 100 mL of sterile distilled water), a solution of Ca(OH)2 + detergent (HCT20)] for S. aureus, E. faecalis, P. aeruginosa, B. subtilis, C. albicans and a mixed culture. Microbial growth was analyzed by two methods: turbidity of the culture medium that was confirmed by Gram stain and subculture in a specific nutrient broth. In the dilution test, NaOCl solution showed MIC equal to 0.1% for S. aureus, E. faecalis, P. aeruginosa and C. albicans and equal to 1% for B. subtilis and the mixed culture. CHX (2%) presented MIC equal to 0.000002% for S. aureus, 0.02% for E. faecalis, B. subtilis, C. albicans and the mixed culture and 0.002% for P. aeruginosa. Ca(OH)2 solution (1%) showed MIC greater than 1% for all the microorganisms except P. aeruginosa for which it was equal to 1%. Calcium hydroxide solution + detergent showed MIC equal to 4.5 mL for S. aureus, P. aeruginosa, B. subtilis, C. albicans and the mixed culture and greater than 4.5 mL for E. faecalis. In the direct exposure test, NaOCl had better antimicrobial effectiveness for all microorganisms at all times. CHX (2%) was effective for S. aureus, E. faecalis and C. albicans at all times, and ineffective for P. aeruginosa, B. subtilis and the mixed culture. The other solutions showed the worst results.http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0103-64402003000300009endodontic irrigantssodium hypochloritechlorhexidine digluconateintracanal dressing |
| spellingShingle | Estrela Cyntia R.A. Estrela Carlos Reis Cleômenes Bammann Lili Luschke Pécora Jesus Djalma Control of microorganisms in vitro by endodontic irrigants Brazilian Dental Journal endodontic irrigants sodium hypochlorite chlorhexidine digluconate intracanal dressing |
| title | Control of microorganisms in vitro by endodontic irrigants |
| title_full | Control of microorganisms in vitro by endodontic irrigants |
| title_fullStr | Control of microorganisms in vitro by endodontic irrigants |
| title_full_unstemmed | Control of microorganisms in vitro by endodontic irrigants |
| title_short | Control of microorganisms in vitro by endodontic irrigants |
| title_sort | control of microorganisms in vitro by endodontic irrigants |
| topic | endodontic irrigants sodium hypochlorite chlorhexidine digluconate intracanal dressing |
| url | http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0103-64402003000300009 |
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