An overview of irritans-mariner transposons in two Mayetiola species (Diptera: Cecidomyiidae)
Mariner-like elements (MLEs) are widespread Class II transposable elements in insects that are subdivided into several subfamilies. In the current study, we carried out in silico analysis and in vitro experiments to identify MLEs belonging to the irritans subfamily in two cecidomyiid flies, Mayetiol...
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| Format: | Article |
| Language: | English |
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Institute of Entomology, Biology Centre, Czech Academy of Science
2017-10-01
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| Series: | European Journal of Entomology |
| Subjects: | |
| Online Access: | https://www.eje.cz/artkey/eje-201701-0049_An_overview_of_irritans-mariner_transposons_in_two_Mayetiola_species_Diptera_Cecidomyiidae.php |
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| author | Wiem BEN AMARA Salma DJEBBI Dhia BOUKTILA Mohamed MAKNI Hanem MAKNI Maha MEZGHANI-KHEMAKHEM |
| author_facet | Wiem BEN AMARA Salma DJEBBI Dhia BOUKTILA Mohamed MAKNI Hanem MAKNI Maha MEZGHANI-KHEMAKHEM |
| author_sort | Wiem BEN AMARA |
| collection | DOAJ |
| description | Mariner-like elements (MLEs) are widespread Class II transposable elements in insects that are subdivided into several subfamilies. In the current study, we carried out in silico analysis and in vitro experiments to identify MLEs belonging to the irritans subfamily in two cecidomyiid flies, Mayetiola destructor and M. hordei. In silico investigation of M. destructor genome allowed the identification of 25 irritans-like elements, which were mostly defective due to several mutations. These defective forms might be the remnants of active elements that ancestrally invaded the host genome. Structural analyses, including signature motifs and transposase-encoding ORFs, revealed structural heterogeneity and the presence of two full length copies. Five consensuses, reflecting the probable evolutionary groups of these elements, were constructed, based on a similarity matrix. The first consensus (Maymarcons1) belonged to Himar1-like elements reported in other insects, while the remaining four (Maymarcons2 to 5) seemed to be more specific to Cecidomyiidae. Moreover, the presence of elements belonging to the Maymarcons4 group was ascertained by PCR amplification, in both Mayetiola species, and was further identified in the Transcriptome Shotgun Assembly (TSA) of the orange fly, Sitodiplosis mosellana (Cecidomyiidae), suggesting the existence of irritans elements within the Cecidomyiidae, which were derived from an ancestral species by vertical transmission during speciation. On the other hand, consensuses that are specific to M. destructor could be derived from a more recent invasion. This study suggests that both M. destructor and M. hordei genomes have been invaded by irritans elements many times with at least two different evolutionary histories. |
| first_indexed | 2024-12-17T23:58:07Z |
| format | Article |
| id | doaj.art-c01d61ff6cc44d9e80bae915c7c45391 |
| institution | Directory Open Access Journal |
| issn | 1210-5759 1802-8829 |
| language | English |
| last_indexed | 2024-12-17T23:58:07Z |
| publishDate | 2017-10-01 |
| publisher | Institute of Entomology, Biology Centre, Czech Academy of Science |
| record_format | Article |
| series | European Journal of Entomology |
| spelling | doaj.art-c01d61ff6cc44d9e80bae915c7c453912022-12-21T21:28:01ZengInstitute of Entomology, Biology Centre, Czech Academy of ScienceEuropean Journal of Entomology1210-57591802-88292017-10-01114137939010.14411/eje.2017.049eje-201701-0049An overview of irritans-mariner transposons in two Mayetiola species (Diptera: Cecidomyiidae)Wiem BEN AMARA0Salma DJEBBI1Dhia BOUKTILA2Mohamed MAKNI3Hanem MAKNI4Maha MEZGHANI-KHEMAKHEM5Unité de Recherche Génomique des Insectes Ravageurs des Cultures d'intérêt agronomique (UR11ES10), Faculté des Sciences de Tunis, Université de Tunis El Manar, 2092 El Manar, Tunis, Tunisia; e-mails: wiem.benamara7@gmail.com, salma.djebbi@fst.utm.tn, dhia_bouktila2000@yahoo.fr, mdmakni98@gmail.com, hanem.makni@isajc.rnu.tn, maha.mezghani@fst.utm.tnUnité de Recherche Génomique des Insectes Ravageurs des Cultures d'intérêt agronomique (UR11ES10), Faculté des Sciences de Tunis, Université de Tunis El Manar, 2092 El Manar, Tunis, Tunisia; e-mails: wiem.benamara7@gmail.com, salma.djebbi@fst.utm.tn, dhia_bouktila2000@yahoo.fr, mdmakni98@gmail.com, hanem.makni@isajc.rnu.tn, maha.mezghani@fst.utm.tnUnité de Recherche Génomique des Insectes Ravageurs des Cultures d'intérêt agronomique (UR11ES10), Faculté des Sciences de Tunis, Université de Tunis El Manar, 2092 El Manar, Tunis, Tunisia; e-mails: wiem.benamara7@gmail.com, salma.djebbi@fst.utm.tn, dhia_bouktila2000@yahoo.fr, mdmakni98@gmail.com, hanem.makni@isajc.rnu.tn, maha.mezghani@fst.utm.tnUnité de Recherche Génomique des Insectes Ravageurs des Cultures d'intérêt agronomique (UR11ES10), Faculté des Sciences de Tunis, Université de Tunis El Manar, 2092 El Manar, Tunis, Tunisia; e-mails: wiem.benamara7@gmail.com, salma.djebbi@fst.utm.tn, dhia_bouktila2000@yahoo.fr, mdmakni98@gmail.com, hanem.makni@isajc.rnu.tn, maha.mezghani@fst.utm.tnUnité de Recherche Génomique des Insectes Ravageurs des Cultures d'intérêt agronomique (UR11ES10), Faculté des Sciences de Tunis, Université de Tunis El Manar, 2092 El Manar, Tunis, Tunisia; e-mails: wiem.benamara7@gmail.com, salma.djebbi@fst.utm.tn, dhia_bouktila2000@yahoo.fr, mdmakni98@gmail.com, hanem.makni@isajc.rnu.tn, maha.mezghani@fst.utm.tnUnité de Recherche Génomique des Insectes Ravageurs des Cultures d'intérêt agronomique (UR11ES10), Faculté des Sciences de Tunis, Université de Tunis El Manar, 2092 El Manar, Tunis, Tunisia; e-mails: wiem.benamara7@gmail.com, salma.djebbi@fst.utm.tn, dhia_bouktila2000@yahoo.fr, mdmakni98@gmail.com, hanem.makni@isajc.rnu.tn, maha.mezghani@fst.utm.tnMariner-like elements (MLEs) are widespread Class II transposable elements in insects that are subdivided into several subfamilies. In the current study, we carried out in silico analysis and in vitro experiments to identify MLEs belonging to the irritans subfamily in two cecidomyiid flies, Mayetiola destructor and M. hordei. In silico investigation of M. destructor genome allowed the identification of 25 irritans-like elements, which were mostly defective due to several mutations. These defective forms might be the remnants of active elements that ancestrally invaded the host genome. Structural analyses, including signature motifs and transposase-encoding ORFs, revealed structural heterogeneity and the presence of two full length copies. Five consensuses, reflecting the probable evolutionary groups of these elements, were constructed, based on a similarity matrix. The first consensus (Maymarcons1) belonged to Himar1-like elements reported in other insects, while the remaining four (Maymarcons2 to 5) seemed to be more specific to Cecidomyiidae. Moreover, the presence of elements belonging to the Maymarcons4 group was ascertained by PCR amplification, in both Mayetiola species, and was further identified in the Transcriptome Shotgun Assembly (TSA) of the orange fly, Sitodiplosis mosellana (Cecidomyiidae), suggesting the existence of irritans elements within the Cecidomyiidae, which were derived from an ancestral species by vertical transmission during speciation. On the other hand, consensuses that are specific to M. destructor could be derived from a more recent invasion. This study suggests that both M. destructor and M. hordei genomes have been invaded by irritans elements many times with at least two different evolutionary histories.https://www.eje.cz/artkey/eje-201701-0049_An_overview_of_irritans-mariner_transposons_in_two_Mayetiola_species_Diptera_Cecidomyiidae.phpdipteracecidomyiidaemayetiola destructormayetiola hordeisitodiplosis mosellanairritans subfamilyin silico analysisin vitro experimentsmariner-like elementtransposons |
| spellingShingle | Wiem BEN AMARA Salma DJEBBI Dhia BOUKTILA Mohamed MAKNI Hanem MAKNI Maha MEZGHANI-KHEMAKHEM An overview of irritans-mariner transposons in two Mayetiola species (Diptera: Cecidomyiidae) European Journal of Entomology diptera cecidomyiidae mayetiola destructor mayetiola hordei sitodiplosis mosellana irritans subfamily in silico analysis in vitro experiments mariner-like element transposons |
| title | An overview of irritans-mariner transposons in two Mayetiola species (Diptera: Cecidomyiidae) |
| title_full | An overview of irritans-mariner transposons in two Mayetiola species (Diptera: Cecidomyiidae) |
| title_fullStr | An overview of irritans-mariner transposons in two Mayetiola species (Diptera: Cecidomyiidae) |
| title_full_unstemmed | An overview of irritans-mariner transposons in two Mayetiola species (Diptera: Cecidomyiidae) |
| title_short | An overview of irritans-mariner transposons in two Mayetiola species (Diptera: Cecidomyiidae) |
| title_sort | overview of irritans mariner transposons in two mayetiola species diptera cecidomyiidae |
| topic | diptera cecidomyiidae mayetiola destructor mayetiola hordei sitodiplosis mosellana irritans subfamily in silico analysis in vitro experiments mariner-like element transposons |
| url | https://www.eje.cz/artkey/eje-201701-0049_An_overview_of_irritans-mariner_transposons_in_two_Mayetiola_species_Diptera_Cecidomyiidae.php |
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