Anti-metastatic Profiles of Boesenbergia pandurata towards MCF-7/HER2 Cells
The development of breast cancer at an advanced stage is signed with metastatic phenomenon, triggering the high mortality, mainly for Human Epidermal Growth Factor Receptor (HER)2 positive cancers. Boesenbergia pandurata is well known as medicinal plant possessing anticancer potential due to the cyt...
| Main Authors: | , , , , , |
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| Format: | Article |
| Language: | English |
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Indonesian Society for Cancer Chemoprevention
2018-07-01
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| Series: | ISCC (Indonesian Journal of Cancer Chemoprevention) |
| Online Access: | https://ijcc.chemoprev.org/index.php/ijcc/article/view/217 |
| _version_ | 1828336547987980288 |
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| author | Hilyatul Fadliyah Nindya Budiana Putri Ziana Walidah Ika Putri Nurhayati Muthi Ikawati Edy Meiyanto |
| author_facet | Hilyatul Fadliyah Nindya Budiana Putri Ziana Walidah Ika Putri Nurhayati Muthi Ikawati Edy Meiyanto |
| author_sort | Hilyatul Fadliyah |
| collection | DOAJ |
| description | The development of breast cancer at an advanced stage is signed with metastatic phenomenon, triggering the high mortality, mainly for Human Epidermal Growth Factor Receptor (HER)2 positive cancers. Boesenbergia pandurata is well known as medicinal plant possessing anticancer potential due to the cytototoxic and antimetastatic characteristic of its active compound. The aim of this study is to observe the inhibitory effect of Boesenbergia pandurata ethanolic extract (BPEE) in combination with doxorubicin on migration of MCF-7/HER2 cells. The BPEE was prepared by 96% ethanol maceration. Under MTT assay, BPEE decreased the cells viability with IC50 value of 23±3.9 μg/mL. Lamellipodia and wound healing assay analysis showed that 5 μg/mL BPPE and its combination with 10 nM doxorubicin inhibited cells migration after 48 hours observation, while gelatin zymography analysis showed that this combination did not affect the expression of Matrix Metalloproteinase (MMP)2 and MMP9, but single treatment of 5 μg/mL BPEE caused lower expression of both MMPs. The combination of 5 μg/mL BPPE and 10 nM doxorubicin inhibited the cells migration but not affect to the cells viability. Thus, BPEE is potential to be developed as an antimetastatic agent. The mechanism underlying the migratory inhibition effect needs to be explored further.
Keywords : Boesenbergia pandurata, doxorubicin, MCF-7/HER2, migration
Submitted: |
| first_indexed | 2024-04-13T22:02:26Z |
| format | Article |
| id | doaj.art-c01f5fc59a174351b5455d5b2a6737ec |
| institution | Directory Open Access Journal |
| issn | 2088-0197 2355-8989 |
| language | English |
| last_indexed | 2024-04-13T22:02:26Z |
| publishDate | 2018-07-01 |
| publisher | Indonesian Society for Cancer Chemoprevention |
| record_format | Article |
| series | ISCC (Indonesian Journal of Cancer Chemoprevention) |
| spelling | doaj.art-c01f5fc59a174351b5455d5b2a6737ec2022-12-22T02:28:03ZengIndonesian Society for Cancer ChemopreventionISCC (Indonesian Journal of Cancer Chemoprevention)2088-01972355-89892018-07-0192687710.14499/indonesianjcanchemoprev9iss2pp68-77164Anti-metastatic Profiles of Boesenbergia pandurata towards MCF-7/HER2 CellsHilyatul Fadliyah0Nindya Budiana Putri1Ziana Walidah2Ika Putri Nurhayati3Muthi Ikawati4Edy Meiyanto5Cancer Chemoprevention Research Center, Faculty of Pharmacy, Universitas Gadjah MadaCancer Chemoprevention Research Center, Faculty of Pharmacy, Universitas Gadjah Mada, IndonesiaCancer Chemoprevention Research Center, Faculty of Pharmacy, Universitas Gadjah Mada, IndonesiaDepartement of Pharmacy, Faculty of Medicine, Universitas Brawijaya, IndonesiaDepartement of Pharmaceutical Chemistry, Faculty of Pharmacy, Universitas Gadjah Mada, IndonesiaDepartement of Pharmaceutical Chemistry, Faculty of Pharmacy, Universitas Gadjah Mada, IndonesiaThe development of breast cancer at an advanced stage is signed with metastatic phenomenon, triggering the high mortality, mainly for Human Epidermal Growth Factor Receptor (HER)2 positive cancers. Boesenbergia pandurata is well known as medicinal plant possessing anticancer potential due to the cytototoxic and antimetastatic characteristic of its active compound. The aim of this study is to observe the inhibitory effect of Boesenbergia pandurata ethanolic extract (BPEE) in combination with doxorubicin on migration of MCF-7/HER2 cells. The BPEE was prepared by 96% ethanol maceration. Under MTT assay, BPEE decreased the cells viability with IC50 value of 23±3.9 μg/mL. Lamellipodia and wound healing assay analysis showed that 5 μg/mL BPPE and its combination with 10 nM doxorubicin inhibited cells migration after 48 hours observation, while gelatin zymography analysis showed that this combination did not affect the expression of Matrix Metalloproteinase (MMP)2 and MMP9, but single treatment of 5 μg/mL BPEE caused lower expression of both MMPs. The combination of 5 μg/mL BPPE and 10 nM doxorubicin inhibited the cells migration but not affect to the cells viability. Thus, BPEE is potential to be developed as an antimetastatic agent. The mechanism underlying the migratory inhibition effect needs to be explored further. Keywords : Boesenbergia pandurata, doxorubicin, MCF-7/HER2, migration Submitted:https://ijcc.chemoprev.org/index.php/ijcc/article/view/217 |
| spellingShingle | Hilyatul Fadliyah Nindya Budiana Putri Ziana Walidah Ika Putri Nurhayati Muthi Ikawati Edy Meiyanto Anti-metastatic Profiles of Boesenbergia pandurata towards MCF-7/HER2 Cells ISCC (Indonesian Journal of Cancer Chemoprevention) |
| title | Anti-metastatic Profiles of Boesenbergia pandurata towards MCF-7/HER2 Cells |
| title_full | Anti-metastatic Profiles of Boesenbergia pandurata towards MCF-7/HER2 Cells |
| title_fullStr | Anti-metastatic Profiles of Boesenbergia pandurata towards MCF-7/HER2 Cells |
| title_full_unstemmed | Anti-metastatic Profiles of Boesenbergia pandurata towards MCF-7/HER2 Cells |
| title_short | Anti-metastatic Profiles of Boesenbergia pandurata towards MCF-7/HER2 Cells |
| title_sort | anti metastatic profiles of boesenbergia pandurata towards mcf 7 her2 cells |
| url | https://ijcc.chemoprev.org/index.php/ijcc/article/view/217 |
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