Chromatin regulators as capacitors of interspecies variations in gene expression
Gene expression varies widely between closely related species and strains, yet the genetic basis of most differences is still unknown. Several studies suggested that chromatin regulators have a key role in generating expression diversity, predicting a reduction in the interspecies differences on del...
| Main Authors: | , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Springer Nature
2010-01-01
|
| Series: | Molecular Systems Biology |
| Subjects: | |
| Online Access: | https://doi.org/10.1038/msb.2010.84 |
| _version_ | 1797279706645528576 |
|---|---|
| author | Itay Tirosh Sharon Reikhav Nadejda Sigal Yael Assia Naama Barkai |
| author_facet | Itay Tirosh Sharon Reikhav Nadejda Sigal Yael Assia Naama Barkai |
| author_sort | Itay Tirosh |
| collection | DOAJ |
| description | Gene expression varies widely between closely related species and strains, yet the genetic basis of most differences is still unknown. Several studies suggested that chromatin regulators have a key role in generating expression diversity, predicting a reduction in the interspecies differences on deletion of genes that influence chromatin structure or modifications. To examine this, we compared the genome‐wide expression profiles of two closely related yeast species following the individual deletions of eight chromatin regulators and one transcription factor. In all cases, regulator deletions increased, rather than decreased, the expression differences between the species, revealing hidden genetic variability that was masked in the wild‐type backgrounds. This effect was not observed for individual deletions of 11 enzymes involved in central metabolic pathways. The buffered variations were associated with trans differences, as revealed by allele‐specific profiling of the interspecific hybrids. Our results support the idea that regulatory proteins serve as capacitors that buffer gene expression against hidden genetic variability. |
| first_indexed | 2024-03-07T16:29:52Z |
| format | Article |
| id | doaj.art-c021fdebcfd14c8088b2ba972f0d4c8e |
| institution | Directory Open Access Journal |
| issn | 1744-4292 |
| language | English |
| last_indexed | 2024-03-07T16:29:52Z |
| publishDate | 2010-01-01 |
| publisher | Springer Nature |
| record_format | Article |
| series | Molecular Systems Biology |
| spelling | doaj.art-c021fdebcfd14c8088b2ba972f0d4c8e2024-03-03T11:26:08ZengSpringer NatureMolecular Systems Biology1744-42922010-01-0161n/an/a10.1038/msb.2010.84Chromatin regulators as capacitors of interspecies variations in gene expressionItay Tirosh0Sharon Reikhav1Nadejda Sigal2Yael Assia3Naama Barkai4Department of Molecular Genetics, Weizmann Institute of Science Rehovot IsraelDepartment of Molecular Genetics, Weizmann Institute of Science Rehovot IsraelDepartment of Molecular Genetics, Weizmann Institute of Science Rehovot IsraelDepartment of Molecular Genetics, Weizmann Institute of Science Rehovot IsraelDepartment of Molecular Genetics, Weizmann Institute of Science Rehovot IsraelGene expression varies widely between closely related species and strains, yet the genetic basis of most differences is still unknown. Several studies suggested that chromatin regulators have a key role in generating expression diversity, predicting a reduction in the interspecies differences on deletion of genes that influence chromatin structure or modifications. To examine this, we compared the genome‐wide expression profiles of two closely related yeast species following the individual deletions of eight chromatin regulators and one transcription factor. In all cases, regulator deletions increased, rather than decreased, the expression differences between the species, revealing hidden genetic variability that was masked in the wild‐type backgrounds. This effect was not observed for individual deletions of 11 enzymes involved in central metabolic pathways. The buffered variations were associated with trans differences, as revealed by allele‐specific profiling of the interspecific hybrids. Our results support the idea that regulatory proteins serve as capacitors that buffer gene expression against hidden genetic variability.https://doi.org/10.1038/msb.2010.84chromatin structureevolutiongene expressiongenetic capacitor |
| spellingShingle | Itay Tirosh Sharon Reikhav Nadejda Sigal Yael Assia Naama Barkai Chromatin regulators as capacitors of interspecies variations in gene expression Molecular Systems Biology chromatin structure evolution gene expression genetic capacitor |
| title | Chromatin regulators as capacitors of interspecies variations in gene expression |
| title_full | Chromatin regulators as capacitors of interspecies variations in gene expression |
| title_fullStr | Chromatin regulators as capacitors of interspecies variations in gene expression |
| title_full_unstemmed | Chromatin regulators as capacitors of interspecies variations in gene expression |
| title_short | Chromatin regulators as capacitors of interspecies variations in gene expression |
| title_sort | chromatin regulators as capacitors of interspecies variations in gene expression |
| topic | chromatin structure evolution gene expression genetic capacitor |
| url | https://doi.org/10.1038/msb.2010.84 |
| work_keys_str_mv | AT itaytirosh chromatinregulatorsascapacitorsofinterspeciesvariationsingeneexpression AT sharonreikhav chromatinregulatorsascapacitorsofinterspeciesvariationsingeneexpression AT nadejdasigal chromatinregulatorsascapacitorsofinterspeciesvariationsingeneexpression AT yaelassia chromatinregulatorsascapacitorsofinterspeciesvariationsingeneexpression AT naamabarkai chromatinregulatorsascapacitorsofinterspeciesvariationsingeneexpression |