The Impact of Phytases on the Release of Bioactive Inositols, the Profile of Inositol Phosphates, and the Release of Selected Minerals in the Technology of Buckwheat Beer Production
A relatively high concentration of phytate in buckwheat malt, and the low activity of endogenous buckwheat phytases, both of which limit the effective use of substrates (starch, proteins, minerals) for fermentation and yeast metabolism, gives rise to the potential for application of phytases in beer...
| Main Authors: | , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
MDPI AG
2020-01-01
|
| Series: | Biomolecules |
| Subjects: | |
| Online Access: | https://www.mdpi.com/2218-273X/10/2/166 |
| _version_ | 1811308773623463936 |
|---|---|
| author | Robert Duliński Marek Zdaniewicz Aneta Pater Dagmara Poniewska Krzysztof Żyła |
| author_facet | Robert Duliński Marek Zdaniewicz Aneta Pater Dagmara Poniewska Krzysztof Żyła |
| author_sort | Robert Duliński |
| collection | DOAJ |
| description | A relatively high concentration of phytate in buckwheat malt, and the low activity of endogenous buckwheat phytases, both of which limit the effective use of substrates (starch, proteins, minerals) for fermentation and yeast metabolism, gives rise to the potential for application of phytases in beer production. This study aims at obtaining a 100% buckwheat wort with high bioactive cyclitols (<i>myo</i>-inositol and D-<i>chiro</i>-inositol) concentrations released by exogenous phytases and acid phosphatases. Two mashing programs were used in the study, i.e., (1) typical for basic raw materials, namely the well-established Congress method, and (2) optimized for phytase activity. The results indicated a nearly 50% increase in the level of bioactive <i>myo</i>-inositol and an 80% degradation of phytate in the wort as a result of simultaneous application of phytase and phosphatase enzymes in the mashing of buckwheat malt. In addition, high D-<i>chiro</i>-inositol concentrations were released from malt to the buckwheat wort. The concerted action of the two phytases significantly increased (19−44%) Zn<sup>2+</sup> concentrations in wort. This may be of great importance during mash fermentation by <i>Saccharomyces cerevisiae</i> yeasts. There is a potential to develop technology for buckwheat beer production, which, in addition to being free from gluten, comprises high levels of bioactive <i>myo-</i> and <i>D-chiro</i>-inositols. |
| first_indexed | 2024-04-13T09:30:27Z |
| format | Article |
| id | doaj.art-c022dc176be84e32a589de026b5ea847 |
| institution | Directory Open Access Journal |
| issn | 2218-273X |
| language | English |
| last_indexed | 2024-04-13T09:30:27Z |
| publishDate | 2020-01-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Biomolecules |
| spelling | doaj.art-c022dc176be84e32a589de026b5ea8472022-12-22T02:52:18ZengMDPI AGBiomolecules2218-273X2020-01-0110216610.3390/biom10020166biom10020166The Impact of Phytases on the Release of Bioactive Inositols, the Profile of Inositol Phosphates, and the Release of Selected Minerals in the Technology of Buckwheat Beer ProductionRobert Duliński0Marek Zdaniewicz1Aneta Pater2Dagmara Poniewska3Krzysztof Żyła4Department of Biotechnology and General Technology of Food, Faculty of Food Technology, Agricultural University in Krakow, Balicka 122, 30-149 Krakow, PolandDepartment of Fermentation Technology and Microbiology, Faculty of Food Technology, Agricultural University in Krakow, Balicka 122, 30-149 Krakow, PolandDepartment of Fermentation Technology and Microbiology, Faculty of Food Technology, Agricultural University in Krakow, Balicka 122, 30-149 Krakow, PolandDepartment of Biotechnology and General Technology of Food, Faculty of Food Technology, Agricultural University in Krakow, Balicka 122, 30-149 Krakow, PolandDepartment of Biotechnology and General Technology of Food, Faculty of Food Technology, Agricultural University in Krakow, Balicka 122, 30-149 Krakow, PolandA relatively high concentration of phytate in buckwheat malt, and the low activity of endogenous buckwheat phytases, both of which limit the effective use of substrates (starch, proteins, minerals) for fermentation and yeast metabolism, gives rise to the potential for application of phytases in beer production. This study aims at obtaining a 100% buckwheat wort with high bioactive cyclitols (<i>myo</i>-inositol and D-<i>chiro</i>-inositol) concentrations released by exogenous phytases and acid phosphatases. Two mashing programs were used in the study, i.e., (1) typical for basic raw materials, namely the well-established Congress method, and (2) optimized for phytase activity. The results indicated a nearly 50% increase in the level of bioactive <i>myo</i>-inositol and an 80% degradation of phytate in the wort as a result of simultaneous application of phytase and phosphatase enzymes in the mashing of buckwheat malt. In addition, high D-<i>chiro</i>-inositol concentrations were released from malt to the buckwheat wort. The concerted action of the two phytases significantly increased (19−44%) Zn<sup>2+</sup> concentrations in wort. This may be of great importance during mash fermentation by <i>Saccharomyces cerevisiae</i> yeasts. There is a potential to develop technology for buckwheat beer production, which, in addition to being free from gluten, comprises high levels of bioactive <i>myo-</i> and <i>D-chiro</i>-inositols.https://www.mdpi.com/2218-273X/10/2/166d-<i>chiro</i>-inositol<i>myo</i>-inositolbuckwheat beerhpaec-padinositol phosphates profile |
| spellingShingle | Robert Duliński Marek Zdaniewicz Aneta Pater Dagmara Poniewska Krzysztof Żyła The Impact of Phytases on the Release of Bioactive Inositols, the Profile of Inositol Phosphates, and the Release of Selected Minerals in the Technology of Buckwheat Beer Production Biomolecules d-<i>chiro</i>-inositol <i>myo</i>-inositol buckwheat beer hpaec-pad inositol phosphates profile |
| title | The Impact of Phytases on the Release of Bioactive Inositols, the Profile of Inositol Phosphates, and the Release of Selected Minerals in the Technology of Buckwheat Beer Production |
| title_full | The Impact of Phytases on the Release of Bioactive Inositols, the Profile of Inositol Phosphates, and the Release of Selected Minerals in the Technology of Buckwheat Beer Production |
| title_fullStr | The Impact of Phytases on the Release of Bioactive Inositols, the Profile of Inositol Phosphates, and the Release of Selected Minerals in the Technology of Buckwheat Beer Production |
| title_full_unstemmed | The Impact of Phytases on the Release of Bioactive Inositols, the Profile of Inositol Phosphates, and the Release of Selected Minerals in the Technology of Buckwheat Beer Production |
| title_short | The Impact of Phytases on the Release of Bioactive Inositols, the Profile of Inositol Phosphates, and the Release of Selected Minerals in the Technology of Buckwheat Beer Production |
| title_sort | impact of phytases on the release of bioactive inositols the profile of inositol phosphates and the release of selected minerals in the technology of buckwheat beer production |
| topic | d-<i>chiro</i>-inositol <i>myo</i>-inositol buckwheat beer hpaec-pad inositol phosphates profile |
| url | https://www.mdpi.com/2218-273X/10/2/166 |
| work_keys_str_mv | AT robertdulinski theimpactofphytasesonthereleaseofbioactiveinositolstheprofileofinositolphosphatesandthereleaseofselectedmineralsinthetechnologyofbuckwheatbeerproduction AT marekzdaniewicz theimpactofphytasesonthereleaseofbioactiveinositolstheprofileofinositolphosphatesandthereleaseofselectedmineralsinthetechnologyofbuckwheatbeerproduction AT anetapater theimpactofphytasesonthereleaseofbioactiveinositolstheprofileofinositolphosphatesandthereleaseofselectedmineralsinthetechnologyofbuckwheatbeerproduction AT dagmaraponiewska theimpactofphytasesonthereleaseofbioactiveinositolstheprofileofinositolphosphatesandthereleaseofselectedmineralsinthetechnologyofbuckwheatbeerproduction AT krzysztofzyła theimpactofphytasesonthereleaseofbioactiveinositolstheprofileofinositolphosphatesandthereleaseofselectedmineralsinthetechnologyofbuckwheatbeerproduction AT robertdulinski impactofphytasesonthereleaseofbioactiveinositolstheprofileofinositolphosphatesandthereleaseofselectedmineralsinthetechnologyofbuckwheatbeerproduction AT marekzdaniewicz impactofphytasesonthereleaseofbioactiveinositolstheprofileofinositolphosphatesandthereleaseofselectedmineralsinthetechnologyofbuckwheatbeerproduction AT anetapater impactofphytasesonthereleaseofbioactiveinositolstheprofileofinositolphosphatesandthereleaseofselectedmineralsinthetechnologyofbuckwheatbeerproduction AT dagmaraponiewska impactofphytasesonthereleaseofbioactiveinositolstheprofileofinositolphosphatesandthereleaseofselectedmineralsinthetechnologyofbuckwheatbeerproduction AT krzysztofzyła impactofphytasesonthereleaseofbioactiveinositolstheprofileofinositolphosphatesandthereleaseofselectedmineralsinthetechnologyofbuckwheatbeerproduction |