Oncogenic miR-19a and miR-19b co-regulate tumor suppressor MTUS1 to promote cell proliferation and migration in lung cancer
ABSTRACT MTUS1 (microtubule-associated tumor suppressor 1) has been identified that can function as a tumor suppressor gene in many malignant tumors. However, the function and mechanisms underlying the regulation of MTUS1 are unclear. In the present study, we reported that miR-19a and miR-19b (miR-1...
| Main Authors: | , , , , , , , , , , , , |
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| Format: | Article |
| Language: | English |
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Oxford University Press
2017-03-01
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| Series: | Protein & Cell |
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| Online Access: | http://link.springer.com/article/10.1007/s13238-017-0393-7 |
| _version_ | 1797758251180228608 |
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| author | Yuanyuan Gu Shuoxin Liu Xiaodan Zhang Guimin Chen Hongwei Liang Mengchao Yu Zhicong Liao Yong Zhou Chen-Yu Zhang Tao Wang Chen Wang Junfeng Zhang Xi Chen |
| author_facet | Yuanyuan Gu Shuoxin Liu Xiaodan Zhang Guimin Chen Hongwei Liang Mengchao Yu Zhicong Liao Yong Zhou Chen-Yu Zhang Tao Wang Chen Wang Junfeng Zhang Xi Chen |
| author_sort | Yuanyuan Gu |
| collection | DOAJ |
| description | ABSTRACT MTUS1 (microtubule-associated tumor suppressor 1) has been identified that can function as a tumor suppressor gene in many malignant tumors. However, the function and mechanisms underlying the regulation of MTUS1 are unclear. In the present study, we reported that miR-19a and miR-19b (miR-19a/b) promote proliferation and migration of lung cancer cells by targeting MTUS1. First, MTUS1 was proved to function as a tumor suppressor in lung cancer and was linked to cell proliferation and migration promotion. Second, an inverse correlation between miR-19a/b expression and MTUS1 mRNA/protein expression was noted in human lung cancer tissues. Third, MTUS1 was appraised as a direct target of miR-19a/b by bioinformatics analysis. Fourth, direct MTUS1 regulation by miR-19a/b in lung cancer cells was experimentally affirmed by cell transfection assay and luciferase reporter assay. Finally, miR-19a/b were shown to cooperatively repress MTUS1 expression and synergistically regulate MTUS1 expression to promote lung cancer cell proliferation and migration. In conclusion, our findings have provided the first clues regarding the roles of miR-19a/b, which appear to function as oncomirs in lung cancer by downregulating MTUS1. |
| first_indexed | 2024-03-12T18:27:24Z |
| format | Article |
| id | doaj.art-c0232d646a7244a890a9725181b03bc6 |
| institution | Directory Open Access Journal |
| issn | 1674-800X 1674-8018 |
| language | English |
| last_indexed | 2024-03-12T18:27:24Z |
| publishDate | 2017-03-01 |
| publisher | Oxford University Press |
| record_format | Article |
| series | Protein & Cell |
| spelling | doaj.art-c0232d646a7244a890a9725181b03bc62023-08-02T08:29:08ZengOxford University PressProtein & Cell1674-800X1674-80182017-03-018645546610.1007/s13238-017-0393-7Oncogenic miR-19a and miR-19b co-regulate tumor suppressor MTUS1 to promote cell proliferation and migration in lung cancerYuanyuan Gu0Shuoxin Liu1Xiaodan Zhang2Guimin Chen3Hongwei Liang4Mengchao Yu5Zhicong Liao6Yong Zhou7Chen-Yu Zhang8Tao Wang9Chen Wang10Junfeng Zhang11Xi Chen12State Key Laboratory of Pharmaceutical Biotechnology, Jiangsu Engineering Research Center for Micro, RNA Biology and Biotechnology, NJU Advanced Institute for Life Sciences (NAILS), School of Life Sciences, Nanjing UniversityThe Second Department of Medical Oncology, Linyi Tumor HospitalState Key Laboratory of Pharmaceutical Biotechnology, Jiangsu Engineering Research Center for Micro, RNA Biology and Biotechnology, NJU Advanced Institute for Life Sciences (NAILS), School of Life Sciences, Nanjing UniversityThe Second Department of Medical Oncology, Linyi Tumor HospitalState Key Laboratory of Pharmaceutical Biotechnology, Jiangsu Engineering Research Center for Micro, RNA Biology and Biotechnology, NJU Advanced Institute for Life Sciences (NAILS), School of Life Sciences, Nanjing UniversityState Key Laboratory of Pharmaceutical Biotechnology, Jiangsu Engineering Research Center for Micro, RNA Biology and Biotechnology, NJU Advanced Institute for Life Sciences (NAILS), School of Life Sciences, Nanjing UniversityDepartment of Cardio-Thoracic Surgery, Nanjing Drum Tower Hospital Affiliated to Medical School of Nanjing University and Nanjing Multi-Center BiobankDepartment of Cardio-Thoracic Surgery, Nanjing Drum Tower Hospital Affiliated to Medical School of Nanjing University and Nanjing Multi-Center BiobankState Key Laboratory of Pharmaceutical Biotechnology, Jiangsu Engineering Research Center for Micro, RNA Biology and Biotechnology, NJU Advanced Institute for Life Sciences (NAILS), School of Life Sciences, Nanjing UniversityState Key Laboratory of Pharmaceutical Biotechnology, Jiangsu Engineering Research Center for Micro, RNA Biology and Biotechnology, NJU Advanced Institute for Life Sciences (NAILS), School of Life Sciences, Nanjing UniversityState Key Laboratory of Pharmaceutical Biotechnology, Jiangsu Engineering Research Center for Micro, RNA Biology and Biotechnology, NJU Advanced Institute for Life Sciences (NAILS), School of Life Sciences, Nanjing UniversityState Key Laboratory of Pharmaceutical Biotechnology, Jiangsu Engineering Research Center for Micro, RNA Biology and Biotechnology, NJU Advanced Institute for Life Sciences (NAILS), School of Life Sciences, Nanjing UniversityState Key Laboratory of Pharmaceutical Biotechnology, Jiangsu Engineering Research Center for Micro, RNA Biology and Biotechnology, NJU Advanced Institute for Life Sciences (NAILS), School of Life Sciences, Nanjing UniversityABSTRACT MTUS1 (microtubule-associated tumor suppressor 1) has been identified that can function as a tumor suppressor gene in many malignant tumors. However, the function and mechanisms underlying the regulation of MTUS1 are unclear. In the present study, we reported that miR-19a and miR-19b (miR-19a/b) promote proliferation and migration of lung cancer cells by targeting MTUS1. First, MTUS1 was proved to function as a tumor suppressor in lung cancer and was linked to cell proliferation and migration promotion. Second, an inverse correlation between miR-19a/b expression and MTUS1 mRNA/protein expression was noted in human lung cancer tissues. Third, MTUS1 was appraised as a direct target of miR-19a/b by bioinformatics analysis. Fourth, direct MTUS1 regulation by miR-19a/b in lung cancer cells was experimentally affirmed by cell transfection assay and luciferase reporter assay. Finally, miR-19a/b were shown to cooperatively repress MTUS1 expression and synergistically regulate MTUS1 expression to promote lung cancer cell proliferation and migration. In conclusion, our findings have provided the first clues regarding the roles of miR-19a/b, which appear to function as oncomirs in lung cancer by downregulating MTUS1.http://link.springer.com/article/10.1007/s13238-017-0393-7microRNAMTUS1miR-19a/blung cancerproliferationmigration |
| spellingShingle | Yuanyuan Gu Shuoxin Liu Xiaodan Zhang Guimin Chen Hongwei Liang Mengchao Yu Zhicong Liao Yong Zhou Chen-Yu Zhang Tao Wang Chen Wang Junfeng Zhang Xi Chen Oncogenic miR-19a and miR-19b co-regulate tumor suppressor MTUS1 to promote cell proliferation and migration in lung cancer Protein & Cell microRNA MTUS1 miR-19a/b lung cancer proliferation migration |
| title | Oncogenic miR-19a and miR-19b co-regulate tumor suppressor MTUS1 to promote cell proliferation and migration in lung cancer |
| title_full | Oncogenic miR-19a and miR-19b co-regulate tumor suppressor MTUS1 to promote cell proliferation and migration in lung cancer |
| title_fullStr | Oncogenic miR-19a and miR-19b co-regulate tumor suppressor MTUS1 to promote cell proliferation and migration in lung cancer |
| title_full_unstemmed | Oncogenic miR-19a and miR-19b co-regulate tumor suppressor MTUS1 to promote cell proliferation and migration in lung cancer |
| title_short | Oncogenic miR-19a and miR-19b co-regulate tumor suppressor MTUS1 to promote cell proliferation and migration in lung cancer |
| title_sort | oncogenic mir 19a and mir 19b co regulate tumor suppressor mtus1 to promote cell proliferation and migration in lung cancer |
| topic | microRNA MTUS1 miR-19a/b lung cancer proliferation migration |
| url | http://link.springer.com/article/10.1007/s13238-017-0393-7 |
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