Angiotensin II-accelerated vulnerability of carotid plaque in a cholesterol-fed rabbit model-assessed with magnetic resonance imaging comparing to histopathology
This study sought to reveal the effect of angiotensin II (Ang II)-induced atherosclerotic vulnerability in rabbits and to determine whether in vivo magnetic resonance imaging (MRI) can determine the effect of Ang II on atherosclerotic development over time. In total, 24 elderly male New Zealand whit...
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| Format: | Article |
| Language: | English |
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Elsevier
2017-03-01
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| Series: | Saudi Journal of Biological Sciences |
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| Online Access: | http://www.sciencedirect.com/science/article/pii/S1319562X17300268 |
| _version_ | 1798044595905363968 |
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| author | Beibei Sun Huilin Zhao Xiao Li Hong Yao Xiaosheng Liu Qing Lu Jieqing Wan Jianrong Xu |
| author_facet | Beibei Sun Huilin Zhao Xiao Li Hong Yao Xiaosheng Liu Qing Lu Jieqing Wan Jianrong Xu |
| author_sort | Beibei Sun |
| collection | DOAJ |
| description | This study sought to reveal the effect of angiotensin II (Ang II)-induced atherosclerotic vulnerability in rabbits and to determine whether in vivo magnetic resonance imaging (MRI) can determine the effect of Ang II on atherosclerotic development over time. In total, 24 elderly male New Zealand white rabbits underwent an intravascular balloon injury in the left common carotid artery (LCCA) and were subsequently fed a high cholesterol diet for 12 weeks. At 8 weeks, rabbits were randomly assigned to receive either Ang II (1.4 mg/kg/d, Ang II group) or vehicle (phosphate-buffered saline, control) via a subcutaneous osmotic minipump for 4 weeks. The rabbits were imaged three times: at baseline and at 8 and 12 weeks. After the 12-week MRI scanning, rabbits were euthanized to obtain pathological and histological data. Atherosclerotic plaques were identified in the 21 rabbits that survived the 12-week trial. Typical feature of vulnerable plaques (VP), intraplaque hemorrhage, were observed in 6 of 10 animals (60.0%) in the Ang II group. The Cohen K value of MR imaging between the AHA classifications was 0.82 (0.73–0.91; P < 0.001). MRI revealed that the change in carotid morphology were significantly different between the Ang II and control group plaques. Our results support an important role for Ang II in plaque vulnerability by promoting intraplaque neovascularization and hemorrhage as well as inflammation. The vulnerable features induced by Ang II in rabbit carotid plaques could be accurately monitored with MRI in vivo and confirmed with histomorphology. |
| first_indexed | 2024-04-11T23:06:37Z |
| format | Article |
| id | doaj.art-c02558b662c14cc8a0fa99d2097a961d |
| institution | Directory Open Access Journal |
| issn | 1319-562X |
| language | English |
| last_indexed | 2024-04-11T23:06:37Z |
| publishDate | 2017-03-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Saudi Journal of Biological Sciences |
| spelling | doaj.art-c02558b662c14cc8a0fa99d2097a961d2022-12-22T03:58:00ZengElsevierSaudi Journal of Biological Sciences1319-562X2017-03-0124349550310.1016/j.sjbs.2017.01.017Angiotensin II-accelerated vulnerability of carotid plaque in a cholesterol-fed rabbit model-assessed with magnetic resonance imaging comparing to histopathologyBeibei Sun0Huilin Zhao1Xiao Li2Hong Yao3Xiaosheng Liu4Qing Lu5Jieqing Wan6Jianrong Xu7Department of Radiology, Renji Hospital, School of Medicine, Shanghai Jiaotong University, Shanghai 200127, ChinaDepartment of Radiology, Renji Hospital, School of Medicine, Shanghai Jiaotong University, Shanghai 200127, ChinaDepartment of Radiology, Renji Hospital, School of Medicine, Shanghai Jiaotong University, Shanghai 200127, ChinaDepartment of Neurosurgery, Renji Hospital, School of Medicine, Shanghai Jiaotong University, Shanghai 200127, ChinaDepartment of Radiology, Renji Hospital, School of Medicine, Shanghai Jiaotong University, Shanghai 200127, ChinaDepartment of Radiology, Renji Hospital, School of Medicine, Shanghai Jiaotong University, Shanghai 200127, ChinaDepartment of Neurosurgery, Renji Hospital, School of Medicine, Shanghai Jiaotong University, Shanghai 200127, ChinaDepartment of Radiology, Renji Hospital, School of Medicine, Shanghai Jiaotong University, Shanghai 200127, ChinaThis study sought to reveal the effect of angiotensin II (Ang II)-induced atherosclerotic vulnerability in rabbits and to determine whether in vivo magnetic resonance imaging (MRI) can determine the effect of Ang II on atherosclerotic development over time. In total, 24 elderly male New Zealand white rabbits underwent an intravascular balloon injury in the left common carotid artery (LCCA) and were subsequently fed a high cholesterol diet for 12 weeks. At 8 weeks, rabbits were randomly assigned to receive either Ang II (1.4 mg/kg/d, Ang II group) or vehicle (phosphate-buffered saline, control) via a subcutaneous osmotic minipump for 4 weeks. The rabbits were imaged three times: at baseline and at 8 and 12 weeks. After the 12-week MRI scanning, rabbits were euthanized to obtain pathological and histological data. Atherosclerotic plaques were identified in the 21 rabbits that survived the 12-week trial. Typical feature of vulnerable plaques (VP), intraplaque hemorrhage, were observed in 6 of 10 animals (60.0%) in the Ang II group. The Cohen K value of MR imaging between the AHA classifications was 0.82 (0.73–0.91; P < 0.001). MRI revealed that the change in carotid morphology were significantly different between the Ang II and control group plaques. Our results support an important role for Ang II in plaque vulnerability by promoting intraplaque neovascularization and hemorrhage as well as inflammation. The vulnerable features induced by Ang II in rabbit carotid plaques could be accurately monitored with MRI in vivo and confirmed with histomorphology.http://www.sciencedirect.com/science/article/pii/S1319562X17300268Angiotensin IIRabbit modelVulnerabilityMagnetic resonance imagingCarotid artery |
| spellingShingle | Beibei Sun Huilin Zhao Xiao Li Hong Yao Xiaosheng Liu Qing Lu Jieqing Wan Jianrong Xu Angiotensin II-accelerated vulnerability of carotid plaque in a cholesterol-fed rabbit model-assessed with magnetic resonance imaging comparing to histopathology Saudi Journal of Biological Sciences Angiotensin II Rabbit model Vulnerability Magnetic resonance imaging Carotid artery |
| title | Angiotensin II-accelerated vulnerability of carotid plaque in a cholesterol-fed rabbit model-assessed with magnetic resonance imaging comparing to histopathology |
| title_full | Angiotensin II-accelerated vulnerability of carotid plaque in a cholesterol-fed rabbit model-assessed with magnetic resonance imaging comparing to histopathology |
| title_fullStr | Angiotensin II-accelerated vulnerability of carotid plaque in a cholesterol-fed rabbit model-assessed with magnetic resonance imaging comparing to histopathology |
| title_full_unstemmed | Angiotensin II-accelerated vulnerability of carotid plaque in a cholesterol-fed rabbit model-assessed with magnetic resonance imaging comparing to histopathology |
| title_short | Angiotensin II-accelerated vulnerability of carotid plaque in a cholesterol-fed rabbit model-assessed with magnetic resonance imaging comparing to histopathology |
| title_sort | angiotensin ii accelerated vulnerability of carotid plaque in a cholesterol fed rabbit model assessed with magnetic resonance imaging comparing to histopathology |
| topic | Angiotensin II Rabbit model Vulnerability Magnetic resonance imaging Carotid artery |
| url | http://www.sciencedirect.com/science/article/pii/S1319562X17300268 |
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