Discovery of EST-Derived Microsatellite Primers in the Legume <i>Lens culinaris</i> (Fabaceae)
Premise of the study: We developed microsatellite markers in the legume <i>Lens culinaris</i> from publicly available databases to enrich the limited marker resource available for the crop. Methods and Results: Eighty-two primer sets were identified using expressed sequence sets of <...
| Main Authors: | , , , , |
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| Format: | Article |
| Language: | English |
| Published: |
Wiley
2013-06-01
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| Series: | Applications in Plant Sciences |
| Subjects: | |
| Online Access: | http://www.bioone.org/doi/full/10.3732/apps.1200539 |
| _version_ | 1818512765843668992 |
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| author | Neelu Jain H. K. Dikshit D. Singh Akanksha Singh Harish Kumar |
| author_facet | Neelu Jain H. K. Dikshit D. Singh Akanksha Singh Harish Kumar |
| author_sort | Neelu Jain |
| collection | DOAJ |
| description | Premise of the study: We developed microsatellite markers in the legume <i>Lens culinaris</i> from publicly available databases to enrich the limited marker resource available for the crop.
Methods and Results: Eighty-two primer sets were identified using expressed sequence sets of <i>L. culinaris</i> available in the National Center for Biotechnology Information (NCBI) database and were characterized in six species of <i>Lens</i>. Among them, 20 simple sequence repeat (SSR) primers produced no amplification product, 43 produced monomorphic products, and 19 were polymorphic. The primers amplified mono-, di-, tri-, tetra-, penta-, and hexanucleotide repeats with one to four alleles. These SSR loci successfully amplified in five related wild species, with a total of 61 primer pairs in <i>L. nigricans</i> and <i>L. odemensis</i> (98.39%), 59 in <i>L. tomentosus</i> (95.1 %), and 60 in <i>L. ervoides</i> and <i>L. orientalis</i> (96.7%), respectively.
Conclusions: The microsatellite markers discovered in this study will be useful in genetic mapping, marker-assisted breeding, and characterization of germplasm. |
| first_indexed | 2024-12-10T23:51:38Z |
| format | Article |
| id | doaj.art-c026e3b994984b2ca8b5cdacf7d81a30 |
| institution | Directory Open Access Journal |
| issn | 2168-0450 |
| language | English |
| last_indexed | 2024-12-10T23:51:38Z |
| publishDate | 2013-06-01 |
| publisher | Wiley |
| record_format | Article |
| series | Applications in Plant Sciences |
| spelling | doaj.art-c026e3b994984b2ca8b5cdacf7d81a302022-12-22T01:28:44ZengWileyApplications in Plant Sciences2168-04502013-06-0117120053910.3732/apps.1200539Discovery of EST-Derived Microsatellite Primers in the Legume <i>Lens culinaris</i> (Fabaceae)Neelu Jain0H. K. Dikshit1D. Singh2Akanksha Singh3Harish Kumar4Division of Genetics, Indian Agricultural Research Institute, New Delhi 110012, IndiaDivision of Genetics, Indian Agricultural Research Institute, New Delhi 110012, IndiaDivision of Genetics, Indian Agricultural Research Institute, New Delhi 110012, IndiaDivision of Genetics, Indian Agricultural Research Institute, New Delhi 110012, IndiaDivision of Genetics, Indian Agricultural Research Institute, New Delhi 110012, IndiaPremise of the study: We developed microsatellite markers in the legume <i>Lens culinaris</i> from publicly available databases to enrich the limited marker resource available for the crop. Methods and Results: Eighty-two primer sets were identified using expressed sequence sets of <i>L. culinaris</i> available in the National Center for Biotechnology Information (NCBI) database and were characterized in six species of <i>Lens</i>. Among them, 20 simple sequence repeat (SSR) primers produced no amplification product, 43 produced monomorphic products, and 19 were polymorphic. The primers amplified mono-, di-, tri-, tetra-, penta-, and hexanucleotide repeats with one to four alleles. These SSR loci successfully amplified in five related wild species, with a total of 61 primer pairs in <i>L. nigricans</i> and <i>L. odemensis</i> (98.39%), 59 in <i>L. tomentosus</i> (95.1 %), and 60 in <i>L. ervoides</i> and <i>L. orientalis</i> (96.7%), respectively. Conclusions: The microsatellite markers discovered in this study will be useful in genetic mapping, marker-assisted breeding, and characterization of germplasm.http://www.bioone.org/doi/full/10.3732/apps.1200539EST-SSRsFabaceaeLens culinarismicrosatellites |
| spellingShingle | Neelu Jain H. K. Dikshit D. Singh Akanksha Singh Harish Kumar Discovery of EST-Derived Microsatellite Primers in the Legume <i>Lens culinaris</i> (Fabaceae) Applications in Plant Sciences EST-SSRs Fabaceae Lens culinaris microsatellites |
| title | Discovery of EST-Derived Microsatellite Primers in the Legume <i>Lens culinaris</i> (Fabaceae) |
| title_full | Discovery of EST-Derived Microsatellite Primers in the Legume <i>Lens culinaris</i> (Fabaceae) |
| title_fullStr | Discovery of EST-Derived Microsatellite Primers in the Legume <i>Lens culinaris</i> (Fabaceae) |
| title_full_unstemmed | Discovery of EST-Derived Microsatellite Primers in the Legume <i>Lens culinaris</i> (Fabaceae) |
| title_short | Discovery of EST-Derived Microsatellite Primers in the Legume <i>Lens culinaris</i> (Fabaceae) |
| title_sort | discovery of est derived microsatellite primers in the legume i lens culinaris i fabaceae |
| topic | EST-SSRs Fabaceae Lens culinaris microsatellites |
| url | http://www.bioone.org/doi/full/10.3732/apps.1200539 |
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