Description of an in vivo model for the assessment of eosinophil chemoattractants in the mouse
Chemokines (chemoattractant cytokines) induce potent and selective chemotaxis of leukocyte subsets in vitro. Here, we review briefly the chemokines shown to induce eosinophil chemotaxis in vitro and describe a novel model for the study of the ability of chemokines to stimulate eosinophil migration i...
| Main Authors: | , , |
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| Format: | Article |
| Language: | English |
| Published: |
Fundação Oswaldo Cruz (FIOCRUZ)
1997-12-01
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| Series: | Memorias do Instituto Oswaldo Cruz |
| Subjects: | |
| Online Access: | http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0074-02761997000800029 |
| _version_ | 1797757915126300672 |
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| author | Mauro M Teixeira Timothy J Williams Paul G Hellewell |
| author_facet | Mauro M Teixeira Timothy J Williams Paul G Hellewell |
| author_sort | Mauro M Teixeira |
| collection | DOAJ |
| description | Chemokines (chemoattractant cytokines) induce potent and selective chemotaxis of leukocyte subsets in vitro. Here, we review briefly the chemokines shown to induce eosinophil chemotaxis in vitro and describe a novel model for the study of the ability of chemokines to stimulate eosinophil migration in vivo. Eosinophils were purified from the blood of mice over-expressing the IL-5 gene and labelled with 111In. Only the C-C chemokines, eotaxin and MIP-1alpha, but not RANTES, MCP-1, MCP-3, MCP-4, MIP-1ß, KC and MIP-2, effectively induced the recruitment of 111In-eosinophils in mouse skin. We suggest that this mouse model will be useful in assessing the role of endogenously-generated chemokines in mediating eosinophil migration to sites of allergic inflammation in vivo. |
| first_indexed | 2024-03-12T18:22:25Z |
| format | Article |
| id | doaj.art-c02792c0fe3e4adc85ff38f7ca50cbb6 |
| institution | Directory Open Access Journal |
| issn | 0074-0276 1678-8060 |
| language | English |
| last_indexed | 2024-03-12T18:22:25Z |
| publishDate | 1997-12-01 |
| publisher | Fundação Oswaldo Cruz (FIOCRUZ) |
| record_format | Article |
| series | Memorias do Instituto Oswaldo Cruz |
| spelling | doaj.art-c02792c0fe3e4adc85ff38f7ca50cbb62023-08-02T08:46:56ZengFundação Oswaldo Cruz (FIOCRUZ)Memorias do Instituto Oswaldo Cruz0074-02761678-80601997-12-019221121410.1590/S0074-02761997000800029Description of an in vivo model for the assessment of eosinophil chemoattractants in the mouseMauro M TeixeiraTimothy J WilliamsPaul G HellewellChemokines (chemoattractant cytokines) induce potent and selective chemotaxis of leukocyte subsets in vitro. Here, we review briefly the chemokines shown to induce eosinophil chemotaxis in vitro and describe a novel model for the study of the ability of chemokines to stimulate eosinophil migration in vivo. Eosinophils were purified from the blood of mice over-expressing the IL-5 gene and labelled with 111In. Only the C-C chemokines, eotaxin and MIP-1alpha, but not RANTES, MCP-1, MCP-3, MCP-4, MIP-1ß, KC and MIP-2, effectively induced the recruitment of 111In-eosinophils in mouse skin. We suggest that this mouse model will be useful in assessing the role of endogenously-generated chemokines in mediating eosinophil migration to sites of allergic inflammation in vivo.http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0074-02761997000800029chemokineseosinophilseotaxinrecruitment |
| spellingShingle | Mauro M Teixeira Timothy J Williams Paul G Hellewell Description of an in vivo model for the assessment of eosinophil chemoattractants in the mouse Memorias do Instituto Oswaldo Cruz chemokines eosinophils eotaxin recruitment |
| title | Description of an in vivo model for the assessment of eosinophil chemoattractants in the mouse |
| title_full | Description of an in vivo model for the assessment of eosinophil chemoattractants in the mouse |
| title_fullStr | Description of an in vivo model for the assessment of eosinophil chemoattractants in the mouse |
| title_full_unstemmed | Description of an in vivo model for the assessment of eosinophil chemoattractants in the mouse |
| title_short | Description of an in vivo model for the assessment of eosinophil chemoattractants in the mouse |
| title_sort | description of an in vivo model for the assessment of eosinophil chemoattractants in the mouse |
| topic | chemokines eosinophils eotaxin recruitment |
| url | http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0074-02761997000800029 |
| work_keys_str_mv | AT mauromteixeira descriptionofaninvivomodelfortheassessmentofeosinophilchemoattractantsinthemouse AT timothyjwilliams descriptionofaninvivomodelfortheassessmentofeosinophilchemoattractantsinthemouse AT paulghellewell descriptionofaninvivomodelfortheassessmentofeosinophilchemoattractantsinthemouse |