New formulation of a recombinant anthrax vaccine stabilised with structurally modified plant viruses
Anthrax is a disease caused by Bacillus anthracis. The most promising approach to the development of anthrax vaccine is use of the anthrax protective antigen (PA). At the same time, recombinant PA is a very unstable protein. Previously, the authors have designed a stable modified recombinant anthrax...
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| Format: | Article |
| Language: | English |
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Frontiers Media S.A.
2022-09-01
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| Series: | Frontiers in Microbiology |
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| Online Access: | https://www.frontiersin.org/articles/10.3389/fmicb.2022.1003969/full |
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| author | Dmitriy L. Granovskiy Ekaterina M. Ryabchevskaya Ekaterina A. Evtushenko Olga A. Kondakova Marina V. Arkhipenko Tatiana B. Kravchenko Irina V. Bakhteeva Vitalii S. Timofeev Nikolai A. Nikitin Olga V. Karpova |
| author_facet | Dmitriy L. Granovskiy Ekaterina M. Ryabchevskaya Ekaterina A. Evtushenko Olga A. Kondakova Marina V. Arkhipenko Tatiana B. Kravchenko Irina V. Bakhteeva Vitalii S. Timofeev Nikolai A. Nikitin Olga V. Karpova |
| author_sort | Dmitriy L. Granovskiy |
| collection | DOAJ |
| description | Anthrax is a disease caused by Bacillus anthracis. The most promising approach to the development of anthrax vaccine is use of the anthrax protective antigen (PA). At the same time, recombinant PA is a very unstable protein. Previously, the authors have designed a stable modified recombinant anthrax protective antigen with inactivated proteolytic sites and substituted deamidation sites (rPA83m). As a second approach to recombinant PA stabilisation, plant virus spherical particles (SPs) were used as a stabiliser. The combination of these two approaches was shown to be the most effective. Here, the authors report the results of a detailed study of the stability, immunogenicity and protectiveness of rPA83m + SPs compositions. These compositions were shown to be stable, provided high anti-rPA83m antibody titres in guinea pigs and were able to protect them from a fully virulent 81/1 Bacillus anthracis strain. Given these facts, the formulation of rPA83m + SPs compositions is considered to be a prospective anthrax vaccine candidate. |
| first_indexed | 2024-12-10T11:15:51Z |
| format | Article |
| id | doaj.art-c032d7ead96f40cca3b559631c88aff4 |
| institution | Directory Open Access Journal |
| issn | 1664-302X |
| language | English |
| last_indexed | 2024-12-10T11:15:51Z |
| publishDate | 2022-09-01 |
| publisher | Frontiers Media S.A. |
| record_format | Article |
| series | Frontiers in Microbiology |
| spelling | doaj.art-c032d7ead96f40cca3b559631c88aff42022-12-22T01:51:11ZengFrontiers Media S.A.Frontiers in Microbiology1664-302X2022-09-011310.3389/fmicb.2022.10039691003969New formulation of a recombinant anthrax vaccine stabilised with structurally modified plant virusesDmitriy L. Granovskiy0Ekaterina M. Ryabchevskaya1Ekaterina A. Evtushenko2Olga A. Kondakova3Marina V. Arkhipenko4Tatiana B. Kravchenko5Irina V. Bakhteeva6Vitalii S. Timofeev7Nikolai A. Nikitin8Olga V. Karpova9Department of Virology, Faculty of Biology, Lomonosov Moscow State University, Moscow, RussiaDepartment of Virology, Faculty of Biology, Lomonosov Moscow State University, Moscow, RussiaDepartment of Virology, Faculty of Biology, Lomonosov Moscow State University, Moscow, RussiaDepartment of Virology, Faculty of Biology, Lomonosov Moscow State University, Moscow, RussiaDepartment of Virology, Faculty of Biology, Lomonosov Moscow State University, Moscow, RussiaFederal Budget Institution of Science State Research Center for Applied Microbiology and Biotechnology (FBIS SRCAMB), Obolensk, RussiaFederal Budget Institution of Science State Research Center for Applied Microbiology and Biotechnology (FBIS SRCAMB), Obolensk, RussiaFederal Budget Institution of Science State Research Center for Applied Microbiology and Biotechnology (FBIS SRCAMB), Obolensk, RussiaDepartment of Virology, Faculty of Biology, Lomonosov Moscow State University, Moscow, RussiaDepartment of Virology, Faculty of Biology, Lomonosov Moscow State University, Moscow, RussiaAnthrax is a disease caused by Bacillus anthracis. The most promising approach to the development of anthrax vaccine is use of the anthrax protective antigen (PA). At the same time, recombinant PA is a very unstable protein. Previously, the authors have designed a stable modified recombinant anthrax protective antigen with inactivated proteolytic sites and substituted deamidation sites (rPA83m). As a second approach to recombinant PA stabilisation, plant virus spherical particles (SPs) were used as a stabiliser. The combination of these two approaches was shown to be the most effective. Here, the authors report the results of a detailed study of the stability, immunogenicity and protectiveness of rPA83m + SPs compositions. These compositions were shown to be stable, provided high anti-rPA83m antibody titres in guinea pigs and were able to protect them from a fully virulent 81/1 Bacillus anthracis strain. Given these facts, the formulation of rPA83m + SPs compositions is considered to be a prospective anthrax vaccine candidate.https://www.frontiersin.org/articles/10.3389/fmicb.2022.1003969/fullanthraxprotective antigen (PA)vaccine candidateplant virusadjuvanttobacco mosaic virus |
| spellingShingle | Dmitriy L. Granovskiy Ekaterina M. Ryabchevskaya Ekaterina A. Evtushenko Olga A. Kondakova Marina V. Arkhipenko Tatiana B. Kravchenko Irina V. Bakhteeva Vitalii S. Timofeev Nikolai A. Nikitin Olga V. Karpova New formulation of a recombinant anthrax vaccine stabilised with structurally modified plant viruses Frontiers in Microbiology anthrax protective antigen (PA) vaccine candidate plant virus adjuvant tobacco mosaic virus |
| title | New formulation of a recombinant anthrax vaccine stabilised with structurally modified plant viruses |
| title_full | New formulation of a recombinant anthrax vaccine stabilised with structurally modified plant viruses |
| title_fullStr | New formulation of a recombinant anthrax vaccine stabilised with structurally modified plant viruses |
| title_full_unstemmed | New formulation of a recombinant anthrax vaccine stabilised with structurally modified plant viruses |
| title_short | New formulation of a recombinant anthrax vaccine stabilised with structurally modified plant viruses |
| title_sort | new formulation of a recombinant anthrax vaccine stabilised with structurally modified plant viruses |
| topic | anthrax protective antigen (PA) vaccine candidate plant virus adjuvant tobacco mosaic virus |
| url | https://www.frontiersin.org/articles/10.3389/fmicb.2022.1003969/full |
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