Amoebae-resisting Bacteria Isolated from Human Nasal Swabs by Amoebal Coculture
Amoebae feed on bacteria, and few bacteria can resist their microbicidal ability. Amoebal coculture could therefore be used to selectively grow these amoebae-resisting bacteria (ARB), which may be human pathogens. To isolate new ARB, we performed amoebal coculture from 444 nasal samples. We recovere...
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| Format: | Article |
| Language: | English |
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Centers for Disease Control and Prevention
2004-03-01
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| Series: | Emerging Infectious Diseases |
| Subjects: | |
| Online Access: | https://wwwnc.cdc.gov/eid/article/10/3/02-0792_article |
| _version_ | 1823956867477405696 |
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| author | Gilbert Greub Bernard La Scola Didier Raoult |
| author_facet | Gilbert Greub Bernard La Scola Didier Raoult |
| author_sort | Gilbert Greub |
| collection | DOAJ |
| description | Amoebae feed on bacteria, and few bacteria can resist their microbicidal ability. Amoebal coculture could therefore be used to selectively grow these amoebae-resisting bacteria (ARB), which may be human pathogens. To isolate new ARB, we performed amoebal coculture from 444 nasal samples. We recovered 7 (1.6%) ARB from 444 nasal swabs, including 4 new species provisionally named Candidatus Roseomonas massiliae, C. Rhizobium massiliae, C. Chryseobacterium massiliae, and C. Amoebinatus massiliae. The remaining isolates were closely related to Methylobacterium extorquens, Bosea vestrii, and Achromobacter xylosoxidans. Thus, amoebal coculture allows the recovery of new bacterial species from heavily contaminated samples and might be a valuable approach for the recovery of as-yet unrecognized emerging pathogens from clinical specimens. |
| first_indexed | 2024-12-17T15:51:56Z |
| format | Article |
| id | doaj.art-c03733af4c144dffa8d337cd7bdb5f51 |
| institution | Directory Open Access Journal |
| issn | 1080-6040 1080-6059 |
| language | English |
| last_indexed | 2024-12-17T15:51:56Z |
| publishDate | 2004-03-01 |
| publisher | Centers for Disease Control and Prevention |
| record_format | Article |
| series | Emerging Infectious Diseases |
| spelling | doaj.art-c03733af4c144dffa8d337cd7bdb5f512022-12-21T21:42:22ZengCenters for Disease Control and PreventionEmerging Infectious Diseases1080-60401080-60592004-03-0110347047710.3201/eid1003.020792Amoebae-resisting Bacteria Isolated from Human Nasal Swabs by Amoebal CocultureGilbert GreubBernard La ScolaDidier RaoultAmoebae feed on bacteria, and few bacteria can resist their microbicidal ability. Amoebal coculture could therefore be used to selectively grow these amoebae-resisting bacteria (ARB), which may be human pathogens. To isolate new ARB, we performed amoebal coculture from 444 nasal samples. We recovered 7 (1.6%) ARB from 444 nasal swabs, including 4 new species provisionally named Candidatus Roseomonas massiliae, C. Rhizobium massiliae, C. Chryseobacterium massiliae, and C. Amoebinatus massiliae. The remaining isolates were closely related to Methylobacterium extorquens, Bosea vestrii, and Achromobacter xylosoxidans. Thus, amoebal coculture allows the recovery of new bacterial species from heavily contaminated samples and might be a valuable approach for the recovery of as-yet unrecognized emerging pathogens from clinical specimens.https://wwwnc.cdc.gov/eid/article/10/3/02-0792_articlefree-living amoebaeamoebal cocultureamoebae-resisting bacteriaemerging infectious diseaseemerging human pathogennasal swab |
| spellingShingle | Gilbert Greub Bernard La Scola Didier Raoult Amoebae-resisting Bacteria Isolated from Human Nasal Swabs by Amoebal Coculture Emerging Infectious Diseases free-living amoebae amoebal coculture amoebae-resisting bacteria emerging infectious disease emerging human pathogen nasal swab |
| title | Amoebae-resisting Bacteria Isolated from Human Nasal Swabs by Amoebal Coculture |
| title_full | Amoebae-resisting Bacteria Isolated from Human Nasal Swabs by Amoebal Coculture |
| title_fullStr | Amoebae-resisting Bacteria Isolated from Human Nasal Swabs by Amoebal Coculture |
| title_full_unstemmed | Amoebae-resisting Bacteria Isolated from Human Nasal Swabs by Amoebal Coculture |
| title_short | Amoebae-resisting Bacteria Isolated from Human Nasal Swabs by Amoebal Coculture |
| title_sort | amoebae resisting bacteria isolated from human nasal swabs by amoebal coculture |
| topic | free-living amoebae amoebal coculture amoebae-resisting bacteria emerging infectious disease emerging human pathogen nasal swab |
| url | https://wwwnc.cdc.gov/eid/article/10/3/02-0792_article |
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