Optimization of multiple enzymes production by fermentation using lipid-producing Bacillus sp.
The present study identified the pectinase-producing bacterium isolated from the contaminated broth as Bacillus sp. on 16S rDNA sequence analysis. The bacterium illustrated water-like droplets on the colony grown on the Sabouraud dextrose agar plate. It also exhibited multi-enzymes activities, such...
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| Format: | Article |
| Language: | English |
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Frontiers Media S.A.
2022-11-01
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| Series: | Frontiers in Microbiology |
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| Online Access: | https://www.frontiersin.org/articles/10.3389/fmicb.2022.1049692/full |
| _version_ | 1811256290058436608 |
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| author | Sarita Shrestha Chonlong Chio Janak Raj Khatiwada Aristide Laurel Mokale Kognou Wensheng Qin |
| author_facet | Sarita Shrestha Chonlong Chio Janak Raj Khatiwada Aristide Laurel Mokale Kognou Wensheng Qin |
| author_sort | Sarita Shrestha |
| collection | DOAJ |
| description | The present study identified the pectinase-producing bacterium isolated from the contaminated broth as Bacillus sp. on 16S rDNA sequence analysis. The bacterium illustrated water-like droplets on the colony grown on the Sabouraud dextrose agar plate. It also exhibited multi-enzymes activities, such as pectinase, polygalacturonase, xylanase, and cellulase by using various agro-wastes as low-cost substrates. The orange peel was observed to be the best substrate among the agro-wastes used for maximum multi-enzymes (pectinase, polygalacturonase, xylanase, and cellulase). However, the bacterium demonstrated its capability to produce different enzymes according to the different substrates/agro-wastes used. The Plackett–Burman design was used to determine the essential influencing factors, while the Box Behnken design response surface methodology was for optimizing cultural conditions. At their optimal conditions (40°C incubation temperature, 24 h of incubation period, 1% w/v orange peel, and 2% v/v inoculum volume), the bacterium exhibited the maximum pectinase (9.49 ± 1.25 U/ml) and xylanase (16.27 ± 0.52 U/ml) activities. Furthermore, the study explored the ability of the bacterium to produce bacterial lipids and observed about 25% bacterial lipid content on a dry weight basis. Therefore, the bacterium is a good candidate for producing important multi-enzymes and subsequent agro-waste degradation controlling the environment, and facilitating waste management. Also, the bacterium can be a potential feedstock in producing renewable biofuel. |
| first_indexed | 2024-04-12T17:37:53Z |
| format | Article |
| id | doaj.art-c0379a4e99e6429a98402b44f518ee2b |
| institution | Directory Open Access Journal |
| issn | 1664-302X |
| language | English |
| last_indexed | 2024-04-12T17:37:53Z |
| publishDate | 2022-11-01 |
| publisher | Frontiers Media S.A. |
| record_format | Article |
| series | Frontiers in Microbiology |
| spelling | doaj.art-c0379a4e99e6429a98402b44f518ee2b2022-12-22T03:22:54ZengFrontiers Media S.A.Frontiers in Microbiology1664-302X2022-11-011310.3389/fmicb.2022.10496921049692Optimization of multiple enzymes production by fermentation using lipid-producing Bacillus sp.Sarita ShresthaChonlong ChioJanak Raj KhatiwadaAristide Laurel Mokale KognouWensheng QinThe present study identified the pectinase-producing bacterium isolated from the contaminated broth as Bacillus sp. on 16S rDNA sequence analysis. The bacterium illustrated water-like droplets on the colony grown on the Sabouraud dextrose agar plate. It also exhibited multi-enzymes activities, such as pectinase, polygalacturonase, xylanase, and cellulase by using various agro-wastes as low-cost substrates. The orange peel was observed to be the best substrate among the agro-wastes used for maximum multi-enzymes (pectinase, polygalacturonase, xylanase, and cellulase). However, the bacterium demonstrated its capability to produce different enzymes according to the different substrates/agro-wastes used. The Plackett–Burman design was used to determine the essential influencing factors, while the Box Behnken design response surface methodology was for optimizing cultural conditions. At their optimal conditions (40°C incubation temperature, 24 h of incubation period, 1% w/v orange peel, and 2% v/v inoculum volume), the bacterium exhibited the maximum pectinase (9.49 ± 1.25 U/ml) and xylanase (16.27 ± 0.52 U/ml) activities. Furthermore, the study explored the ability of the bacterium to produce bacterial lipids and observed about 25% bacterial lipid content on a dry weight basis. Therefore, the bacterium is a good candidate for producing important multi-enzymes and subsequent agro-waste degradation controlling the environment, and facilitating waste management. Also, the bacterium can be a potential feedstock in producing renewable biofuel.https://www.frontiersin.org/articles/10.3389/fmicb.2022.1049692/fullBacillus sp.multi-enzymesagro-wastesoptimizationlipid content |
| spellingShingle | Sarita Shrestha Chonlong Chio Janak Raj Khatiwada Aristide Laurel Mokale Kognou Wensheng Qin Optimization of multiple enzymes production by fermentation using lipid-producing Bacillus sp. Frontiers in Microbiology Bacillus sp. multi-enzymes agro-wastes optimization lipid content |
| title | Optimization of multiple enzymes production by fermentation using lipid-producing Bacillus sp. |
| title_full | Optimization of multiple enzymes production by fermentation using lipid-producing Bacillus sp. |
| title_fullStr | Optimization of multiple enzymes production by fermentation using lipid-producing Bacillus sp. |
| title_full_unstemmed | Optimization of multiple enzymes production by fermentation using lipid-producing Bacillus sp. |
| title_short | Optimization of multiple enzymes production by fermentation using lipid-producing Bacillus sp. |
| title_sort | optimization of multiple enzymes production by fermentation using lipid producing bacillus sp |
| topic | Bacillus sp. multi-enzymes agro-wastes optimization lipid content |
| url | https://www.frontiersin.org/articles/10.3389/fmicb.2022.1049692/full |
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