Heterologous Expression and Physicochemical Properties of Plantaricin LPL-1
The present study aimed to express soluble recombinant class IIa bacteriocin in Escherichia coli. The plantaricin LPL-1 gene was cloned into an arabinose promoter, and the C-terminus was fused with the his-tag sequence for expression. The inhibitory activity of recombinant plantaricin LPL-1 against...
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| Format: | Article |
| Language: | English |
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China Food Publishing Company
2023-10-01
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| Series: | Shipin Kexue |
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| Online Access: | https://www.spkx.net.cn/fileup/1002-6630/PDF/2023-44-20-013.pdf |
| _version_ | 1827620843315789824 |
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| author | WANG Yu, WANG Yao, LI Pinglan |
| author_facet | WANG Yu, WANG Yao, LI Pinglan |
| author_sort | WANG Yu, WANG Yao, LI Pinglan |
| collection | DOAJ |
| description | The present study aimed to express soluble recombinant class IIa bacteriocin in Escherichia coli. The plantaricin LPL-1 gene was cloned into an arabinose promoter, and the C-terminus was fused with the his-tag sequence for expression. The inhibitory activity of recombinant plantaricin LPL-1 against Listeria monocytogenes 54002 was used as the response variable. A novel cell factory, E. coil (ΔtrxB + Δgor + ahpCM), suitable for class IIa bacteriocin expression was established by CRISPR gene editing technology. It was derived from E. coil BW25331 by deleting the thioredoxin reductase (trxB) gene and glutathione reductase (gor) gene and mutating the gene encoding peroxidase (ahpC). The recombinant plantaricin LPL-1 was purified by affinity chromatography, and its physicochemical properties were analyzed. The purified plantaricin LPL-1 possessed wide pH stability (2–11), high thermal stability (60–100 ℃), and surfactant stability. The recombinant bacteriocin has wide potential for food industrial applications. |
| first_indexed | 2024-03-09T10:54:37Z |
| format | Article |
| id | doaj.art-c0389dfdca9e4f6c940497dbe6cf7d01 |
| institution | Directory Open Access Journal |
| issn | 1002-6630 |
| language | English |
| last_indexed | 2024-03-09T10:54:37Z |
| publishDate | 2023-10-01 |
| publisher | China Food Publishing Company |
| record_format | Article |
| series | Shipin Kexue |
| spelling | doaj.art-c0389dfdca9e4f6c940497dbe6cf7d012023-12-01T03:19:24ZengChina Food Publishing CompanyShipin Kexue1002-66302023-10-01442010010610.7506/spkx1002-6630-20221130-354Heterologous Expression and Physicochemical Properties of Plantaricin LPL-1WANG Yu, WANG Yao, LI Pinglan0(College of Food Science and Nutritional Engineering, China Agricultural University, Beijing 100083, China)The present study aimed to express soluble recombinant class IIa bacteriocin in Escherichia coli. The plantaricin LPL-1 gene was cloned into an arabinose promoter, and the C-terminus was fused with the his-tag sequence for expression. The inhibitory activity of recombinant plantaricin LPL-1 against Listeria monocytogenes 54002 was used as the response variable. A novel cell factory, E. coil (ΔtrxB + Δgor + ahpCM), suitable for class IIa bacteriocin expression was established by CRISPR gene editing technology. It was derived from E. coil BW25331 by deleting the thioredoxin reductase (trxB) gene and glutathione reductase (gor) gene and mutating the gene encoding peroxidase (ahpC). The recombinant plantaricin LPL-1 was purified by affinity chromatography, and its physicochemical properties were analyzed. The purified plantaricin LPL-1 possessed wide pH stability (2–11), high thermal stability (60–100 ℃), and surfactant stability. The recombinant bacteriocin has wide potential for food industrial applications.https://www.spkx.net.cn/fileup/1002-6630/PDF/2023-44-20-013.pdfclass iia bacteriocins; plantaricin lpl-1; heterologous expression; inclusion body; crispr/cas9 |
| spellingShingle | WANG Yu, WANG Yao, LI Pinglan Heterologous Expression and Physicochemical Properties of Plantaricin LPL-1 Shipin Kexue class iia bacteriocins; plantaricin lpl-1; heterologous expression; inclusion body; crispr/cas9 |
| title | Heterologous Expression and Physicochemical Properties of Plantaricin LPL-1 |
| title_full | Heterologous Expression and Physicochemical Properties of Plantaricin LPL-1 |
| title_fullStr | Heterologous Expression and Physicochemical Properties of Plantaricin LPL-1 |
| title_full_unstemmed | Heterologous Expression and Physicochemical Properties of Plantaricin LPL-1 |
| title_short | Heterologous Expression and Physicochemical Properties of Plantaricin LPL-1 |
| title_sort | heterologous expression and physicochemical properties of plantaricin lpl 1 |
| topic | class iia bacteriocins; plantaricin lpl-1; heterologous expression; inclusion body; crispr/cas9 |
| url | https://www.spkx.net.cn/fileup/1002-6630/PDF/2023-44-20-013.pdf |
| work_keys_str_mv | AT wangyuwangyaolipinglan heterologousexpressionandphysicochemicalpropertiesofplantaricinlpl1 |