Oral Immunization of Chickens With Recombinant Lactobacillus plantarum Vaccine Against Early ALV-J Infection
In this study, a novel oral vaccine of recombinant Lactobacillus plantarum (L. plantarum) containing the gp85 protein was explored, and the effects of this vaccine on the prevention of subgroup J Avian Leukosis Virus (ALV-J) infection were assessed. In the current study, the gp85 protein of ALV-J wa...
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| Format: | Article |
| Language: | English |
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Frontiers Media S.A.
2019-10-01
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| Series: | Frontiers in Immunology |
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| Online Access: | https://www.frontiersin.org/article/10.3389/fimmu.2019.02299/full |
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| author | Shenghua Wang Shenghua Wang Na Geng Dong Zhou Yi Qu Mengke Shi Yuliang Xu Kangping Liu Yongxia Liu Jianzhu Liu |
| author_facet | Shenghua Wang Shenghua Wang Na Geng Dong Zhou Yi Qu Mengke Shi Yuliang Xu Kangping Liu Yongxia Liu Jianzhu Liu |
| author_sort | Shenghua Wang |
| collection | DOAJ |
| description | In this study, a novel oral vaccine of recombinant Lactobacillus plantarum (L. plantarum) containing the gp85 protein was explored, and the effects of this vaccine on the prevention of subgroup J Avian Leukosis Virus (ALV-J) infection were assessed. In the current study, the gp85 protein of ALV-J was expressed on the surface of L. plantarum with the surface-display motif, pgsA, by constructing a shuttle vector pMG36e:pgsA:gp85. Surface localization of the fusion protein was verified by western blotting and flow cytometry. Subsequently, Specific Pathogen Free Hy-Line Brown layer chickens were orally vaccinated with the recombinant L. plantarum and presented with high levels of serum immunoglobulin G (IgG) and secretory immunoglobulin A (sIgA) titers in bile and duodenal-mucosal fluid. After challenged with ALV-J of a 3 × 103 50% tissue culture infective dose (TCID50), serum samples of the chickens were collected and viremia was analyzed. Results showed that, compared to the L. plantarum and PBS control group, the recombinant L. plantarum group showed a significant rise in antibody levels after inoculation, and provide improved protection against ALV-J according to viremia detection. These results indicate that oral immunization with the recombinant L. plantarum provided an effective means for eliciting protective immune response against early ALV-J infection. |
| first_indexed | 2024-12-11T08:59:00Z |
| format | Article |
| id | doaj.art-c03dd9c3bc4646c8a54c747114c6e6d2 |
| institution | Directory Open Access Journal |
| issn | 1664-3224 |
| language | English |
| last_indexed | 2024-12-11T08:59:00Z |
| publishDate | 2019-10-01 |
| publisher | Frontiers Media S.A. |
| record_format | Article |
| series | Frontiers in Immunology |
| spelling | doaj.art-c03dd9c3bc4646c8a54c747114c6e6d22022-12-22T01:13:48ZengFrontiers Media S.A.Frontiers in Immunology1664-32242019-10-011010.3389/fimmu.2019.02299419872Oral Immunization of Chickens With Recombinant Lactobacillus plantarum Vaccine Against Early ALV-J InfectionShenghua Wang0Shenghua Wang1Na Geng2Dong Zhou3Yi Qu4Mengke Shi5Yuliang Xu6Kangping Liu7Yongxia Liu8Jianzhu Liu9College of Veterinary Medicine, Shandong Agricultural University, Tai'an, ChinaShandong Provincial Engineering Technology Research Center of Animal Disease Control and Prevention, Shandong Agricultural University, Tai'an, ChinaResearch Center for Animal Disease Control Engineering, Shandong Agricultural University, Tai'an, ChinaCollege of Veterinary Medicine, Northwest Agriculture and Forestry University, Yangling, ChinaNanjing Entry-Exit Inspection and Quarantine Bureau, Nanjing, ChinaResearch Center for Animal Disease Control Engineering, Shandong Agricultural University, Tai'an, ChinaCollege of Veterinary Medicine, Shandong Agricultural University, Tai'an, ChinaCollege of Veterinary Medicine, Shandong Agricultural University, Tai'an, ChinaResearch Center for Animal Disease Control Engineering, Shandong Agricultural University, Tai'an, ChinaCollege of Veterinary Medicine, Shandong Agricultural University, Tai'an, ChinaIn this study, a novel oral vaccine of recombinant Lactobacillus plantarum (L. plantarum) containing the gp85 protein was explored, and the effects of this vaccine on the prevention of subgroup J Avian Leukosis Virus (ALV-J) infection were assessed. In the current study, the gp85 protein of ALV-J was expressed on the surface of L. plantarum with the surface-display motif, pgsA, by constructing a shuttle vector pMG36e:pgsA:gp85. Surface localization of the fusion protein was verified by western blotting and flow cytometry. Subsequently, Specific Pathogen Free Hy-Line Brown layer chickens were orally vaccinated with the recombinant L. plantarum and presented with high levels of serum immunoglobulin G (IgG) and secretory immunoglobulin A (sIgA) titers in bile and duodenal-mucosal fluid. After challenged with ALV-J of a 3 × 103 50% tissue culture infective dose (TCID50), serum samples of the chickens were collected and viremia was analyzed. Results showed that, compared to the L. plantarum and PBS control group, the recombinant L. plantarum group showed a significant rise in antibody levels after inoculation, and provide improved protection against ALV-J according to viremia detection. These results indicate that oral immunization with the recombinant L. plantarum provided an effective means for eliciting protective immune response against early ALV-J infection.https://www.frontiersin.org/article/10.3389/fimmu.2019.02299/fullALV-Jgp85 proteinLactobacillus plantarumpgsAoral immunization |
| spellingShingle | Shenghua Wang Shenghua Wang Na Geng Dong Zhou Yi Qu Mengke Shi Yuliang Xu Kangping Liu Yongxia Liu Jianzhu Liu Oral Immunization of Chickens With Recombinant Lactobacillus plantarum Vaccine Against Early ALV-J Infection Frontiers in Immunology ALV-J gp85 protein Lactobacillus plantarum pgsA oral immunization |
| title | Oral Immunization of Chickens With Recombinant Lactobacillus plantarum Vaccine Against Early ALV-J Infection |
| title_full | Oral Immunization of Chickens With Recombinant Lactobacillus plantarum Vaccine Against Early ALV-J Infection |
| title_fullStr | Oral Immunization of Chickens With Recombinant Lactobacillus plantarum Vaccine Against Early ALV-J Infection |
| title_full_unstemmed | Oral Immunization of Chickens With Recombinant Lactobacillus plantarum Vaccine Against Early ALV-J Infection |
| title_short | Oral Immunization of Chickens With Recombinant Lactobacillus plantarum Vaccine Against Early ALV-J Infection |
| title_sort | oral immunization of chickens with recombinant lactobacillus plantarum vaccine against early alv j infection |
| topic | ALV-J gp85 protein Lactobacillus plantarum pgsA oral immunization |
| url | https://www.frontiersin.org/article/10.3389/fimmu.2019.02299/full |
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