Amperometric Glucose Biosensor Based on Homopolymer-Chitosan Double Layered Glucose Oxidase Electrode Modified with Zinc Oxide Nanoparticles
GOD was immobilized onto polypyrrole (PPy) or poly(o-anisidine) (POA) coated Pt electrode to construct glucose sensitive biosensor. Because polymer film properties and enzyme activity affect the current response, PPy and POA synthesis conditions and also enzyme immobilization parameters were optimiz...
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| Format: | Article |
| Language: | English |
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Slovenian Chemical Society
2019-12-01
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| Series: | Acta Chimica Slovenica |
| Subjects: | |
| Online Access: | https://journals.matheo.si/index.php/ACSi/article/view/5171 |
| _version_ | 1818890087821213696 |
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| author | Gul Ozyilmaz Ali Tuncay Ozyilmaz Esiye Irem Bayram Ragibe Hulya Akyürekoğlu |
| author_facet | Gul Ozyilmaz Ali Tuncay Ozyilmaz Esiye Irem Bayram Ragibe Hulya Akyürekoğlu |
| author_sort | Gul Ozyilmaz |
| collection | DOAJ |
| description | GOD was immobilized onto polypyrrole (PPy) or poly(o-anisidine) (POA) coated Pt electrode to construct glucose sensitive biosensor. Because polymer film properties and enzyme activity affect the current response, PPy and POA synthesis conditions and also enzyme immobilization parameters were optimized in detail. The optimal monomer concentrations were determined as 25 and 50 mM for PPy and POA, respectively, whereas scan rate was 50 mV/s for both polymer films. In case of immobilization procedure, the optimal Chitosan (Chi), glucose oxidase (GOD) and glutaraldehyde (GAL) concentrations were determined as 0.5%, 2 mg/ml and 0.05% for PPy and 0.5%, 4 mg/ml and 0.075% for POA, respectively. Zinc oxide nanoparticles (ZnONP) were co-immobilized with GOD enzyme and it was revealed that ZnONP modification enhanced the efficiencies of both electrodes in terms of current responses and stabilities. Nyquist diagrams showed that enzyme electrodes were sensitive to glucose molecule and ZnONP modification improved the sensor efficiency. |
| first_indexed | 2024-12-19T17:19:21Z |
| format | Article |
| id | doaj.art-c03de660bda0403da277a7c8e3b13e52 |
| institution | Directory Open Access Journal |
| issn | 1318-0207 1580-3155 |
| language | English |
| last_indexed | 2024-12-19T17:19:21Z |
| publishDate | 2019-12-01 |
| publisher | Slovenian Chemical Society |
| record_format | Article |
| series | Acta Chimica Slovenica |
| spelling | doaj.art-c03de660bda0403da277a7c8e3b13e522022-12-21T20:12:44ZengSlovenian Chemical SocietyActa Chimica Slovenica1318-02071580-31552019-12-0166495095710.17344/acsi.2019.5171758Amperometric Glucose Biosensor Based on Homopolymer-Chitosan Double Layered Glucose Oxidase Electrode Modified with Zinc Oxide NanoparticlesGul Ozyilmaz0Ali Tuncay Ozyilmaz1Esiye Irem Bayram2Ragibe Hulya Akyürekoğlu3The University of Mustafa Kemal, Faculty of Arts & Sciences, Department of Chemistry 31040 Hatay/ TurkeyThe University of Mustafa Kemal, Faculty of Arts & Sciences, Department of Chemistry 31040 Hatay/ TurkeyThe University of Mustafa Kemal, Faculty of Arts & Sciences, Department of Chemistry 31040 Hatay/ TurkeyThe University of Mustafa Kemal, Faculty of Arts & Sciences, Department of Chemistry 31040 Hatay/ TurkeyGOD was immobilized onto polypyrrole (PPy) or poly(o-anisidine) (POA) coated Pt electrode to construct glucose sensitive biosensor. Because polymer film properties and enzyme activity affect the current response, PPy and POA synthesis conditions and also enzyme immobilization parameters were optimized in detail. The optimal monomer concentrations were determined as 25 and 50 mM for PPy and POA, respectively, whereas scan rate was 50 mV/s for both polymer films. In case of immobilization procedure, the optimal Chitosan (Chi), glucose oxidase (GOD) and glutaraldehyde (GAL) concentrations were determined as 0.5%, 2 mg/ml and 0.05% for PPy and 0.5%, 4 mg/ml and 0.075% for POA, respectively. Zinc oxide nanoparticles (ZnONP) were co-immobilized with GOD enzyme and it was revealed that ZnONP modification enhanced the efficiencies of both electrodes in terms of current responses and stabilities. Nyquist diagrams showed that enzyme electrodes were sensitive to glucose molecule and ZnONP modification improved the sensor efficiency.https://journals.matheo.si/index.php/ACSi/article/view/5171amperometric biosensorpolypyrrolepoly(o-anisidine)zinc oxide nanoparticlesglucose oxidase |
| spellingShingle | Gul Ozyilmaz Ali Tuncay Ozyilmaz Esiye Irem Bayram Ragibe Hulya Akyürekoğlu Amperometric Glucose Biosensor Based on Homopolymer-Chitosan Double Layered Glucose Oxidase Electrode Modified with Zinc Oxide Nanoparticles Acta Chimica Slovenica amperometric biosensor polypyrrole poly(o-anisidine) zinc oxide nanoparticles glucose oxidase |
| title | Amperometric Glucose Biosensor Based on Homopolymer-Chitosan Double Layered Glucose Oxidase Electrode Modified with Zinc Oxide Nanoparticles |
| title_full | Amperometric Glucose Biosensor Based on Homopolymer-Chitosan Double Layered Glucose Oxidase Electrode Modified with Zinc Oxide Nanoparticles |
| title_fullStr | Amperometric Glucose Biosensor Based on Homopolymer-Chitosan Double Layered Glucose Oxidase Electrode Modified with Zinc Oxide Nanoparticles |
| title_full_unstemmed | Amperometric Glucose Biosensor Based on Homopolymer-Chitosan Double Layered Glucose Oxidase Electrode Modified with Zinc Oxide Nanoparticles |
| title_short | Amperometric Glucose Biosensor Based on Homopolymer-Chitosan Double Layered Glucose Oxidase Electrode Modified with Zinc Oxide Nanoparticles |
| title_sort | amperometric glucose biosensor based on homopolymer chitosan double layered glucose oxidase electrode modified with zinc oxide nanoparticles |
| topic | amperometric biosensor polypyrrole poly(o-anisidine) zinc oxide nanoparticles glucose oxidase |
| url | https://journals.matheo.si/index.php/ACSi/article/view/5171 |
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