Antiretroviral resistance in individuals presenting therapeutic failure and subtypes of the human immunodeficiency virus type 1 in the Northeast Region of Brazil
This study aimed to analyze human immunodeficiency virus (HIV) mutation profiles related to antiretroviral resistance following therapeutic failure, and the distribution of hiv subtypes in the Northeast Region of Brazil. A total of 576 blood samples from AIDS patients presenting therapeutic failure...
| Main Authors: | , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Fundação Oswaldo Cruz (FIOCRUZ)
2007-11-01
|
| Series: | Memorias do Instituto Oswaldo Cruz |
| Subjects: | |
| Online Access: | http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0074-02762007000700002 |
| _version_ | 1797704751082635264 |
|---|---|
| author | Ana Maria Salustiano Cavalcanti Heloisa Ramos Lacerda Ana Maria de Brito Sirleide Pereira Daniela Medeiros Suelene Oliveira |
| author_facet | Ana Maria Salustiano Cavalcanti Heloisa Ramos Lacerda Ana Maria de Brito Sirleide Pereira Daniela Medeiros Suelene Oliveira |
| author_sort | Ana Maria Salustiano Cavalcanti |
| collection | DOAJ |
| description | This study aimed to analyze human immunodeficiency virus (HIV) mutation profiles related to antiretroviral resistance following therapeutic failure, and the distribution of hiv subtypes in the Northeast Region of Brazil. A total of 576 blood samples from AIDS patients presenting therapeutic failure between 2002 and 2004 were analyzed. The genotyping kit viroSeq® was used to perform viral amplification in order to identify mutations related to hiv pol gene resistance. An index of 91.1% of the patients presented mutations for nucleoside reverse transcriptase inhibitors (nrti), 58.7% for non-nucleoside reverse transcriptase inhibitors (nnrti), and 94.8% for protease inhibitors (pi). The most prevalent mutations were 184V and 215E for nrti, 103N and 190A for nnrti. Most mutations associated with PIs were secondary, but significant frequencies were observed in codons 90 (25.2%), 82 (21.1%), and 30 (16.2%). The resistance index to one class of antiretrovirals was 14%, to two classes of antiretrovirals 61%, and to three classes 18.9%. Subtype B was the most prevalent (82.4%) followed by subtype F (11.8%). The prevalence of mutations related to nrti and nnrti was the same in the two subtypes, but codon analysis related to PI showed a higher frequency of mutations in codon 63 in subtype B and in codon 36 in subtype F. The present study showed that there was a high frequency of primary mutations, which offered resistance to nrti and nnrti. Monitoring patients with treatment failure is an important tool for aiding physicians in rescue therapy. |
| first_indexed | 2024-03-12T05:25:03Z |
| format | Article |
| id | doaj.art-c03e6825c77f4910a6895e1aec69356a |
| institution | Directory Open Access Journal |
| issn | 0074-0276 1678-8060 |
| language | English |
| last_indexed | 2024-03-12T05:25:03Z |
| publishDate | 2007-11-01 |
| publisher | Fundação Oswaldo Cruz (FIOCRUZ) |
| record_format | Article |
| series | Memorias do Instituto Oswaldo Cruz |
| spelling | doaj.art-c03e6825c77f4910a6895e1aec69356a2023-09-03T07:25:00ZengFundação Oswaldo Cruz (FIOCRUZ)Memorias do Instituto Oswaldo Cruz0074-02761678-80602007-11-011027785792Antiretroviral resistance in individuals presenting therapeutic failure and subtypes of the human immunodeficiency virus type 1 in the Northeast Region of BrazilAna Maria Salustiano CavalcantiHeloisa Ramos LacerdaAna Maria de BritoSirleide PereiraDaniela MedeirosSuelene OliveiraThis study aimed to analyze human immunodeficiency virus (HIV) mutation profiles related to antiretroviral resistance following therapeutic failure, and the distribution of hiv subtypes in the Northeast Region of Brazil. A total of 576 blood samples from AIDS patients presenting therapeutic failure between 2002 and 2004 were analyzed. The genotyping kit viroSeq® was used to perform viral amplification in order to identify mutations related to hiv pol gene resistance. An index of 91.1% of the patients presented mutations for nucleoside reverse transcriptase inhibitors (nrti), 58.7% for non-nucleoside reverse transcriptase inhibitors (nnrti), and 94.8% for protease inhibitors (pi). The most prevalent mutations were 184V and 215E for nrti, 103N and 190A for nnrti. Most mutations associated with PIs were secondary, but significant frequencies were observed in codons 90 (25.2%), 82 (21.1%), and 30 (16.2%). The resistance index to one class of antiretrovirals was 14%, to two classes of antiretrovirals 61%, and to three classes 18.9%. Subtype B was the most prevalent (82.4%) followed by subtype F (11.8%). The prevalence of mutations related to nrti and nnrti was the same in the two subtypes, but codon analysis related to PI showed a higher frequency of mutations in codon 63 in subtype B and in codon 36 in subtype F. The present study showed that there was a high frequency of primary mutations, which offered resistance to nrti and nnrti. Monitoring patients with treatment failure is an important tool for aiding physicians in rescue therapy.http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0074-02762007000700002human immunodeficiency virus type 1resistance to drugsgenotypingsubtypesBrazil |
| spellingShingle | Ana Maria Salustiano Cavalcanti Heloisa Ramos Lacerda Ana Maria de Brito Sirleide Pereira Daniela Medeiros Suelene Oliveira Antiretroviral resistance in individuals presenting therapeutic failure and subtypes of the human immunodeficiency virus type 1 in the Northeast Region of Brazil Memorias do Instituto Oswaldo Cruz human immunodeficiency virus type 1 resistance to drugs genotyping subtypes Brazil |
| title | Antiretroviral resistance in individuals presenting therapeutic failure and subtypes of the human immunodeficiency virus type 1 in the Northeast Region of Brazil |
| title_full | Antiretroviral resistance in individuals presenting therapeutic failure and subtypes of the human immunodeficiency virus type 1 in the Northeast Region of Brazil |
| title_fullStr | Antiretroviral resistance in individuals presenting therapeutic failure and subtypes of the human immunodeficiency virus type 1 in the Northeast Region of Brazil |
| title_full_unstemmed | Antiretroviral resistance in individuals presenting therapeutic failure and subtypes of the human immunodeficiency virus type 1 in the Northeast Region of Brazil |
| title_short | Antiretroviral resistance in individuals presenting therapeutic failure and subtypes of the human immunodeficiency virus type 1 in the Northeast Region of Brazil |
| title_sort | antiretroviral resistance in individuals presenting therapeutic failure and subtypes of the human immunodeficiency virus type 1 in the northeast region of brazil |
| topic | human immunodeficiency virus type 1 resistance to drugs genotyping subtypes Brazil |
| url | http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0074-02762007000700002 |
| work_keys_str_mv | AT anamariasalustianocavalcanti antiretroviralresistanceinindividualspresentingtherapeuticfailureandsubtypesofthehumanimmunodeficiencyvirustype1inthenortheastregionofbrazil AT heloisaramoslacerda antiretroviralresistanceinindividualspresentingtherapeuticfailureandsubtypesofthehumanimmunodeficiencyvirustype1inthenortheastregionofbrazil AT anamariadebrito antiretroviralresistanceinindividualspresentingtherapeuticfailureandsubtypesofthehumanimmunodeficiencyvirustype1inthenortheastregionofbrazil AT sirleidepereira antiretroviralresistanceinindividualspresentingtherapeuticfailureandsubtypesofthehumanimmunodeficiencyvirustype1inthenortheastregionofbrazil AT danielamedeiros antiretroviralresistanceinindividualspresentingtherapeuticfailureandsubtypesofthehumanimmunodeficiencyvirustype1inthenortheastregionofbrazil AT sueleneoliveira antiretroviralresistanceinindividualspresentingtherapeuticfailureandsubtypesofthehumanimmunodeficiencyvirustype1inthenortheastregionofbrazil |